CTX-M-65

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Accession	ARO:3001926
CARD Short Name	CTX-M-65
Definition	CTX-M-65 is a beta-lactamase found in Escherichia coli.
AMR Gene Family	CTX-M beta-lactamase
Drug Class	cephalosporin
Resistance Mechanism	antibiotic inactivation
Resistomes with Perfect Matches	Enterobacter cloacae^p, Enterobacter hormaechei^p+wgs, Escherichia coli^g+p+wgs, Escherichia fergusonii^p+wgs, Klebsiella aerogenes^p, Klebsiella pneumoniae^g+p+wgs, Klebsiella quasipneumoniae^wgs, Proteus mirabilis^g+p+wgs+gi, Proteus penneri^wgs, Salmonella enterica^g+p+wgs+gi, Shigella flexneri^wgs, Streptococcus suis^wgs
Resistomes with Sequence Variants	Enterobacter cloacae^p, Enterobacter hormaechei^p+wgs, Escherichia coli^g+p+wgs, Escherichia fergusonii^p+wgs, Klebsiella aerogenes^p, Klebsiella pneumoniae^g+p+wgs, Klebsiella quasipneumoniae^wgs, Proteus mirabilis^g+p+wgs+gi, Proteus penneri^wgs, Salmonella enterica^g+p+wgs+gi, Shigella flexneri^wgs, Streptococcus suis^wgs
Classification	12 ontology terms \| Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + hydrolysis of antibiotic conferring resistance + antibiotic molecule + hydrolysis of beta-lactam antibiotic by serine beta-lactamase + beta-lactam antibiotic + beta-lactamase + class A beta-lactamase + cephalosporin [Drug Class]
Parent Term(s)	1 ontology terms \| Show + CTX-M beta-lactamase [AMR Gene Family]
Sub-Term(s)	2 ontology terms \| Show + avibactam [Adjuvant] is_small_molecule_inhibitor + ARX1796 [Adjuvant] is_small_molecule_inhibitor
Publications	Doi Y, et al. 2008. Antimicrob Agents Chemother 52(3): 1204-1205. Escherichia coli isolate coproducing 16S rRNA Methylase and CTX-M-type extended-spectrum beta-lactamase isolated from an outpatient in the United States. (PMID 18195064)

Resistomes

Prevalence of CTX-M-65 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

Species	NCBI Chromosome	NCBI Plasmid	NCBI WGS	NCBI GI	GRDI-AMR2
Enterobacter cloacae	0%	0.56%	0%	0%	0%
Enterobacter cloacae	0%	0.56%	0%	0%	0%
Enterobacter hormaechei	0%	0.06%	0.3%	0%	0%
Enterobacter hormaechei	0%	0.06%	0.3%	0%	0%
Escherichia coli	0.24%	0.31%	1.32%	0%	0%
Escherichia coli	0.24%	0.33%	1.32%	0%	0%
Escherichia fergusonii	0%	1.07%	14.13%	0%	0%
Escherichia fergusonii	0%	1.07%	14.13%	0%	0%
Klebsiella aerogenes	0%	1.09%	0%	0%	0%
Klebsiella aerogenes	0%	1.09%	0%	0%	0%
Klebsiella pneumoniae	0.18%	2.64%	7.62%	0%	0%
Klebsiella pneumoniae	0.18%	2.65%	7.62%	0%	0%
Klebsiella quasipneumoniae	0%	0%	0.13%	0%	0%
Klebsiella quasipneumoniae	0%	0%	0.13%	0%	0%
Proteus mirabilis	11.93%	1.25%	9.74%	3.7%	0%
Proteus mirabilis	12.84%	1.25%	9.74%	7.41%	0%
Proteus penneri	0%	0%	12.5%	0%	0%
Proteus penneri	0%	0%	12.5%	0%	0%
Salmonella enterica	0.57%	1.7%	1.12%	0.33%	0%
Salmonella enterica	0.57%	1.7%	1.13%	0.33%	0%
Shigella flexneri	0%	0%	0.16%	0%	0%
Shigella flexneri	0%	0%	0.16%	0%	0%
Streptococcus suis	0%	0%	0.1%	0%	0%
Streptococcus suis	0%	0%	0.1%	0%	0%

Show Perfect Only

Detection Models

protein homolog model

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 500

Protein
DNA

>gb|ABN69105.1|+|CTX-M-65 [Escherichia coli]
MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSSGGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAVAAVLKQSETQ
KQLLNQPVEIKPADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGDETFRLDRTEPTLNTAIPGDP
RDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNTTGAASIRAGLPTSWTVGDKTGSGDYGTTNDIAVIWPQGRAPLVLVTYFTQPQ
QNAERRRDVLASAARIIAEGL

>gb|EF418608.1|+|10-885|CTX-M-65 [Escherichia coli]
ATGGTGACAAAGAGAGTGCAACGGATGATGTTCGCGGCGGCGGCGTGCATTCCGCTGCTGCTGGGCAGCGCGCCGCTTTATGCGCAGACG
AGTGCGGTGCAGCAAAAGCTGGCGGCGCTGGAGAAAAGCAGCGGAGGGCGGCTGGGCGTCGCGCTCATCGATACCGCAGATAATACGCAG
GTGCTTTATCGCGGTGATGAACGCTTTCCAATGTGCAGTACCAGTAAAGTTATGGCGGTCGCGGCGGTGCTTAAGCAGAGTGAAACGCAA
AAGCAGCTGCTTAATCAGCCTGTCGAGATCAAGCCTGCCGATCTGGTTAACTACAATCCGATTGCCGAAAAACACGTCAACGGCACAATG
ACGCTGGCAGAACTGAGCGCGGCCGCGTTGCAGTACAGCGACAATACCGCCATGAACAAATTGATTGCCCAGCTCGGTGGCCCGGGAGGC
GTGACGGCTTTTGCCCGCGCGATCGGCGATGAGACGTTTCGTCTGGATCGCACTGAACCTACGCTGAATACCGCCATTCCCGGCGACCCG
AGAGACACCACCACGCCGCGGGCGATGGCGCAGACGTTGCGTCAGCTTACGCTGGGTCATGCGCTGGGCGAAACCCAGCGGGCGCAGTTG
GTGACGTGGCTCAAAGGCAATACGACCGGCGCAGCCAGCATTCGGGCCGGCTTACCGACGTCGTGGACTGTGGGTGATAAGACCGGCAGC
GGCGACTACGGCACCACCAATGATATTGCGGTGATCTGGCCGCAGGGTCGTGCGCCGCTGGTTCTGGTGACCTATTTTACCCAGCCGCAA
CAGAACGCAGAGCGCCGCCGCGATGTGCTGGCTTCAGCGGCGAGAATCATCGCCGAAGGGCTGTAA

Curator Acknowledgements

Curator	Description	Most Recent Edit

CTX-M-65

Prevalence: protein homolog model (view sequences)

Graph