| Accession | ARO:3002859 |
| CARD Short Name | dfrA14 |
| Definition | dfrA14 is an integron-encoded dihydrofolate reductase found in Escherichia coli. |
| AMR Gene Family | trimethoprim resistant dihydrofolate reductase dfr |
| Drug Class | diaminopyrimidine antibiotic |
| Resistance Mechanism | antibiotic target replacement |
| Resistomes with Perfect Matches | Acinetobacter baumanniig, Aeromonas caviaewgs, Aeromonas hydrophilap, Aeromonas veroniiwgs, Citrobacter amalonaticuswgs, Citrobacter freundiip+wgs, Citrobacter koserip+wgs, Citrobacter portucalensisg+wgs, Citrobacter werkmaniiwgs, Citrobacter youngaewgs, Cronobacter sakazakiip, Enterobacter asburiaep+wgs, Enterobacter chengduensiswgs, Enterobacter cloacaeg+p+wgs, Enterobacter hormaecheig+p+wgs, Enterobacter kobeiwgs, Enterobacter roggenkampiip+wgs, Escherichia albertiip, Escherichia colig+p+wgs, Escherichia fergusoniip+wgs, Klebsiella aerogenesp+wgs, Klebsiella michiganensisp+wgs, Klebsiella oxytocap+wgs, Klebsiella pneumoniaeg+p+wgs, Klebsiella quasipneumoniaep+wgs, Leclercia adecarboxylatag+wgs, Morganella morganiip+wgs, Proteus mirabilisp+wgs, Proteus vulgarisp, Providencia rettgeriwgs, Providencia stuartiip+wgs, Pseudomonas aeruginosawgs, Raoultella planticolap+wgs, Salmonella entericag+p+wgs, Serratia marcescenswgs, Shewanella putrefaciensp, Shigella boydiiwgs, Shigella dysenteriaewgs, Shigella flexnerip+wgs, Shigella sonneiwgs, Vibrio choleraewgs |
| Resistomes with Sequence Variants | Acinetobacter baumanniig, Aeromonas caviaewgs+gi, Aeromonas hydrophilap, Aeromonas veroniip+wgs, Citrobacter amalonaticuswgs, Citrobacter freundiip+wgs, Citrobacter koserip+wgs, Citrobacter portucalensisg+wgs, Citrobacter werkmaniiwgs, Citrobacter youngaewgs, Cronobacter sakazakiip, Enterobacter asburiaep+wgs, Enterobacter chengduensiswgs, Enterobacter cloacaeg+p+wgs+gi, Enterobacter hormaecheig+p+wgs+gi, Enterobacter kobeiwgs, Enterobacter roggenkampiip+wgs, Escherichia albertiip, Escherichia colig+p+wgs, Escherichia fergusoniip+wgs, Histophilus somniwgs, Klebsiella aerogenesp+wgs, Klebsiella michiganensisg+p+wgs, Klebsiella oxytocap+wgs, Klebsiella pneumoniaeg+p+wgs, Klebsiella quasipneumoniaep+wgs, Leclercia adecarboxylatag+p+wgs, Morganella morganiip+wgs, Pasteurella multocidawgs, Proteus mirabilisg+p+wgs, Proteus vulgarisp, Providencia rettgeriwgs, Providencia stuartiip+wgs, Pseudomonas aeruginosawgs, Pseudomonas putidap, Raoultella planticolap+wgs, Salmonella entericag+p+wgs+gi, Serratia marcescensp+wgs, Shewanella putrefaciensp, Shigella boydiiwgs, Shigella dysenteriaewgs, Shigella flexnerip+wgs, Shigella sonneip+wgs, Staphylococcus aureuswgs, Vibrio choleraewgs, Yersinia enterocoliticawgs |
| Classification | 9 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic target replacement [Resistance Mechanism] + antibiotic molecule + determinant of antibiotic resistance + diaminopyrimidine antibiotic [Drug Class] + antibiotic target replacement protein + antibiotic resistant dihydrofolate reductase + trimethoprim [Antibiotic] |
| Parent Term(s) | 3 ontology terms | Show + trimethoprim resistant dihydrofolate reductase dfr [AMR Gene Family] + derives_from antibiotic sensitive dihydrofolate reductase + confers_resistance_to_antibiotic trimethoprim [Antibiotic] |
| Publications | Marquez C, et al. 2008. Antimicrob Agents Chemother 52(11): 4153-4154. Recovery of a functional class 2 integron from an Escherichia coli strain mediating a urinary tract infection. (PMID 18794381) |
Prevalence of dfrA14 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Acinetobacter baumannii | 0.18% | 0% | 0% | 0% |
| Aeromonas caviae | 0% | 0% | 1.08% | 33.33% |
| Aeromonas hydrophila | 0% | 2.6% | 0% | 0% |
| Aeromonas veronii | 0% | 3.08% | 1.12% | 0% |
| Citrobacter amalonaticus | 0% | 0% | 9.09% | 0% |
| Citrobacter freundii | 0% | 1.23% | 6.77% | 0% |
| Citrobacter koseri | 0% | 5% | 2.7% | 0% |
| Citrobacter portucalensis | 3.7% | 0% | 4.5% | 0% |
| Citrobacter werkmanii | 0% | 0% | 2.56% | 0% |
| Citrobacter youngae | 0% | 0% | 6.25% | 0% |
| Cronobacter sakazakii | 0% | 2.56% | 0% | 0% |
| Enterobacter asburiae | 0% | 0.83% | 5.53% | 0% |
| Enterobacter chengduensis | 0% | 0% | 12% | 0% |
| Enterobacter cloacae | 1.79% | 6.15% | 12.78% | 10% |
| Enterobacter hormaechei | 1.8% | 2.7% | 16.01% | 3.33% |
| Enterobacter kobei | 0% | 0% | 4.37% | 0% |
| Enterobacter roggenkampii | 0% | 1.45% | 6.12% | 0% |
| Escherichia albertii | 0% | 0.56% | 0% | 0% |
| Escherichia coli | 0.24% | 1.62% | 4.16% | 0% |
| Escherichia fergusonii | 0% | 2.14% | 18.48% | 0% |
| Histophilus somni | 0% | 0% | 31.03% | 0% |
| Klebsiella aerogenes | 0% | 1.09% | 4.24% | 0% |
| Klebsiella michiganensis | 1.61% | 4% | 9.31% | 0% |
| Klebsiella oxytoca | 0% | 4.11% | 5.04% | 0% |
| Klebsiella pneumoniae | 0.18% | 5.27% | 15.83% | 0% |
| Klebsiella quasipneumoniae | 0% | 2.33% | 14.61% | 0% |
| Leclercia adecarboxylata | 7.14% | 4.76% | 2.33% | 0% |
| Morganella morganii | 0% | 5% | 1.23% | 0% |
| Pasteurella multocida | 0% | 0% | 0.37% | 0% |
| Proteus mirabilis | 2.75% | 6.25% | 1.49% | 0% |
| Proteus vulgaris | 0% | 11.11% | 0% | 0% |
| Providencia rettgeri | 0% | 0% | 8.28% | 0% |
| Providencia stuartii | 0% | 11.36% | 6.82% | 0% |
| Pseudomonas aeruginosa | 0% | 0% | 0.17% | 0% |
| Pseudomonas putida | 0% | 4% | 0% | 0% |
| Raoultella planticola | 0% | 4.65% | 5.13% | 0% |
| Salmonella enterica | 0.44% | 2.95% | 1.78% | 0.33% |
| Serratia marcescens | 0% | 1.29% | 2.88% | 0% |
| Shewanella putrefaciens | 0% | 20% | 0% | 0% |
| Shigella boydii | 0% | 0% | 14.44% | 0% |
| Shigella dysenteriae | 0% | 0% | 3.33% | 0% |
| Shigella flexneri | 0% | 9.24% | 8.85% | 0% |
| Shigella sonnei | 0% | 0.97% | 7.3% | 0% |
| Staphylococcus aureus | 0% | 0% | 0.01% | 0% |
| Vibrio cholerae | 0% | 0% | 0.06% | 0% |
| Yersinia enterocolitica | 0% | 0% | 0.91% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 300