| Accession | ARO:3002861 |
| CARD Short Name | dfrA5 |
| Definition | dfrA5 is an integron-encoded dihydrofolate reductase found in Vibrio cholerae. |
| AMR Gene Family | trimethoprim resistant dihydrofolate reductase dfr |
| Drug Class | diaminopyrimidine antibiotic |
| Resistance Mechanism | antibiotic target replacement |
| Resistomes with Perfect Matches | Acinetobacter baumanniiwgs, Aeromonas caviaewgs, Citrobacter freundiiwgs, Citrobacter koseriwgs, Comamonas testosteronigi, Enterobacter hormaecheiwgs, Enterobacter roggenkampiiwgs, Escherichia albertiiwgs, Escherichia colig+p+wgs+gi, Escherichia fergusoniiwgs, Klebsiella aerogeneswgs, Klebsiella michiganensiswgs, Klebsiella pneumoniaep+wgs, Klebsiella quasipneumoniaep+wgs, Proteus mirabiliswgs, Proteus vulgariswgs, Pseudomonas aeruginosawgs, Salmonella entericag+p+wgs, Serratia marcescensp, Shigella boydiiwgs, Shigella flexneriwgs, Shigella sonneiwgs |
| Resistomes with Sequence Variants | Acinetobacter baumanniiwgs, Aeromonas caviaewgs, Citrobacter freundiiwgs, Citrobacter koseriwgs, Comamonas testosteronigi, Enterobacter hormaecheiwgs, Enterobacter roggenkampiiwgs, Escherichia albertiiwgs, Escherichia colig+p+wgs+gi, Escherichia fergusoniiwgs, Klebsiella aerogeneswgs, Klebsiella michiganensiswgs, Klebsiella pneumoniaep+wgs, Klebsiella quasipneumoniaep+wgs, Proteus mirabiliswgs, Proteus vulgariswgs, Pseudomonas aeruginosawgs, Salmonella entericag+p+wgs, Serratia marcescensp, Shigella boydiiwgs, Shigella flexneriwgs, Shigella sonneiwgs |
| Classification | 9 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic target replacement [Resistance Mechanism] + diaminopyrimidine antibiotic [Drug Class] + antibiotic target replacement protein + trimethoprim [Antibiotic] + antibiotic resistant dihydrofolate reductase |
| Parent Term(s) | 3 ontology terms | Show + confers_resistance_to_antibiotic trimethoprim [Antibiotic] + derives_from antibiotic sensitive dihydrofolate reductase + trimethoprim resistant dihydrofolate reductase dfr [AMR Gene Family] |
| Publications | Thungapathra M, et al. 2002. Antimicrob Agents Chemother 46(9): 2948-2955. Occurrence of antibiotic resistance gene cassettes aac(6')-Ib, dfrA5, dfrA12, and ereA2 in class I integrons in non-O1, non-O139 Vibrio cholerae strains in India. (PMID 12183252) |
Prevalence of dfrA5 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 263 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Acinetobacter baumannii | 0% | 0% | 0.06% | 0% |
| Aeromonas caviae | 0% | 0% | 0.9% | 0% |
| Citrobacter freundii | 0% | 0% | 0.25% | 0% |
| Citrobacter koseri | 0% | 0% | 1.11% | 0% |
| Comamonas testosteroni | 0% | 0% | 0% | 100% |
| Enterobacter hormaechei | 0% | 0% | 1.3% | 0% |
| Enterobacter roggenkampii | 0% | 0% | 1.21% | 0% |
| Escherichia albertii | 0% | 0% | 1.15% | 0% |
| Escherichia coli | 0.11% | 0.25% | 1.97% | 0.13% |
| Escherichia fergusonii | 0% | 0% | 3.08% | 0% |
| Klebsiella aerogenes | 0% | 0% | 0.31% | 0% |
| Klebsiella michiganensis | 0% | 0% | 0.32% | 0% |
| Klebsiella pneumoniae | 0% | 0.23% | 1.04% | 0% |
| Klebsiella quasipneumoniae | 0% | 0.54% | 0.18% | 0% |
| Proteus mirabilis | 0% | 0% | 0.69% | 0% |
| Proteus vulgaris | 0% | 0% | 6.67% | 0% |
| Pseudomonas aeruginosa | 0% | 0% | 0.39% | 0% |
| Salmonella enterica | 0.15% | 0.07% | 0.3% | 0% |
| Serratia marcescens | 0% | 1.42% | 0% | 0% |
| Shigella boydii | 0% | 0% | 2.15% | 0% |
| Shigella flexneri | 0% | 0% | 0.33% | 0% |
| Shigella sonnei | 0% | 0% | 1.33% | 0% |
Model Type: protein homolog model
Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.
Bit-score Cut-off (blastP): 300