| Accession | ARO:3002861 |
| Definition | dfrA5 is an integron-encoded dihydrofolate reductase found in Vibrio cholerae |
| AMR Gene Family | trimethoprim resistant dihydrofolate reductase dfr |
| Drug Class | diaminopyrimidine antibiotic |
| Resistance Mechanism | antibiotic target replacement |
| Resistomes | Acinetobacter baumanniiwgs, Enterobacter cloacaewgs, Enterobacter hormaecheiwgs, Escherichia colip+wgs, Klebsiella oxytocawgs, Klebsiella pneumoniaep+wgs, Proteus vulgariswgs, Pseudomonas aeruginosawgs, Pseudomonas fluorescenswgs, Salmonella entericap+wgs, Serratia marcescensp, Shigella flexneriwgs, Shigella sonneiwgs |
| Classification | 10 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic molecule + determinant of antibiotic resistance + antibiotic target replacement [Resistance Mechanism] + antibiotic target replacement protein + diaminopyrimidine antibiotic [Drug Class] + determinant of diaminopyrimidine resistance + trimethoprim [Antibiotic] + antibiotic resistant dihydrofolate reductase |
| Parent Term(s) | 3 ontology terms | Show + confers_resistance_to_antibiotic trimethoprim [Antibiotic] + derives_from antibiotic sensitive dihydrofolate reductase + trimethoprim resistant dihydrofolate reductase dfr [AMR Gene Family] |
| Publications | Thungapathra M, et al. 2002. Antimicrob Agents Chemother 46(9): 2948-2955. Occurrence of antibiotic resistance gene cassettes aac(6')-Ib, dfrA5, dfrA12, and ereA2 in class I integrons in non-O1, non-O139 Vibrio cholerae strains in India. (PMID 12183252) |
Prevalence of dfrA5 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 85 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS |
|---|---|---|---|
| Acinetobacter baumannii | 0% | 0% | 0.07% |
| Enterobacter cloacae | 0% | 0% | 3.61% |
| Enterobacter hormaechei | 0% | 0% | 0.63% |
| Escherichia coli | 0% | 0.06% | 1.8% |
| Klebsiella oxytoca | 0% | 0% | 2.8% |
| Klebsiella pneumoniae | 0% | 0.1% | 1.39% |
| Proteus mirabilis | 0% | 0% | 0% |
| Proteus vulgaris | 0% | 0% | 12.5% |
| Pseudomonas aeruginosa | 0% | 0% | 0.34% |
| Pseudomonas fluorescens | 0% | 0% | 0.3% |
| Salmonella enterica | 0% | 0.24% | 0.05% |
| Serratia marcescens | 0% | 1.64% | 0% |
| Shigella flexneri | 0% | 0% | 0.26% |
| Shigella sonnei | 0% | 0% | 1.1% |
Model Type: protein homolog model
Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.
Bit-score Cut-off (blastP): 300