dfrF

Accession ARO:3002867
DefinitiondfrF is a chromosome-encoded dihydrofolate reductase found in Streptococcus pyogenes
AMR Gene Familytrimethoprim resistant dihydrofolate reductase dfr
Drug Classdiaminopyrimidine antibiotic
Resistance Mechanismantibiotic target replacement
ResistomesEnterococcus faecaliswgs, Enterococcus faeciumwgs, Staphylococcus aureuswgs, Streptococcus agalactiaewgs, Streptococcus anginosuswgs, Streptococcus pyogenesg+wgs
Classification10 ontology terms | Show
Parent Term(s)2 ontology terms | Show
Publications

Bergmann R, et al. 2014. Antimicrob Agents Chemother 58(4): 2281-2288. Factors that cause trimethoprim resistance in Streptococcus pyogenes. (PMID 24492367)

Resistomes

Prevalence of dfrF among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 82 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
Enterococcus faecalis2.7%0%4.01%
Enterococcus faecium36%0%21.98%
Staphylococcus aureus0%0%0.1%
Streptococcus agalactiae0%0%0.1%
Streptococcus anginosus0%0%2.56%
Streptococcus pyogenes0.59%0%0.39%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.

Bit-score Cut-off (blastP): 300


>gb|AAD01868.1|+|dfrF [Enterococcus faecalis]
MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGRKSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRD
VYISGGYGLFKEALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKNELSRFWI


>gb|AF028812|+|393-887|dfrF [Enterococcus faecalis]
ATGATAGGTTTGATTGTTGCGAGGTCAAAGAATAATGTTATAGGCAAGAATGGTAATATACCATGGAAAATAAAGGGAGAACAAAAGCAA
TTTAGAGAGTTAACAACGGGTAATGTGGTTATTATGGGGCGAAAGTCTTATGAAGAAATCGGTCATCCGTTGCCTAATAGAATGAATATT
GTTGTTTCCACCACAACAGAGTATCAAGGAGATAATTTAGTTTCAGTTAAATCATTAGAAGATGCATTATTATTGGCTAAAGGACGAGAT
GTATACATATCTGGTGGATATGGACTATTTAAGGAAGCTTTGCAAATAGTAGATAAAATGTATATCACAGAAGTAGATTTAAATATTGAA
GATGGAGATACATTCTTTCCAGAATTTGATATCAATGATTTTGAAGTTTTGATAGGGGAAACACTTGGTGAGGAAGTGAAATATACGAGA
ACATTTTATGTAAGGAAAAATGAATTGAGTAGATTTTGGATTTAG