| Accession | ARO:3002868 |
| CARD Short Name | dfrG |
| Definition | dfrG is a plasmid-encoded dihydrofolate reductase found in Staphylococcus aureus. |
| AMR Gene Family | trimethoprim resistant dihydrofolate reductase dfr |
| Drug Class | diaminopyrimidine antibiotic |
| Resistance Mechanism | antibiotic target replacement |
| Resistomes with Perfect Matches | Enterococcus aviumwgs, Enterococcus faecalisg+p+wgs+gi, Enterococcus faeciumg+p+wgs+gi, Enterococcus hiraep+wgs, Escherichia coliwgs, Klebsiella aerogeneswgs, Klebsiella pneumoniaewgs, Lactococcus garvieaep, Ligilactobacillus animaliswgs, Listeria innocuag+p, Listeria monocytogenesg+wgs, Staphylococcus arlettaeg+wgs, Staphylococcus aureusg+p+wgs+gi, Staphylococcus epidermidisg+p+wgs, Staphylococcus equorumg, Staphylococcus haemolyticusg+wgs+gi, Staphylococcus hominiswgs, Staphylococcus pasteuriwgs, Staphylococcus pseudintermediusg+wgs+gi, Staphylococcus saprophyticusg+wgs+gi, Staphylococcus warnerig+wgs, Streptococcus agalactiaewgs, Streptococcus pyogenesg+wgs, Streptococcus suisg+wgs |
| Resistomes with Sequence Variants | Enterococcus aviumwgs, Enterococcus faecalisg+p+wgs+gi, Enterococcus faeciumg+p+wgs+gi, Enterococcus hiraep+wgs, Escherichia coliwgs, Jeotgalibaca arthritidisgi, Jeotgalibaca porcigi, Klebsiella aerogeneswgs, Klebsiella pneumoniaewgs, Lactococcus garvieaep, Ligilactobacillus animaliswgs, Listeria innocuag+p, Listeria monocytogenesg+wgs, Staphylococcus arlettaeg+wgs, Staphylococcus aureusg+p+wgs+gi, Staphylococcus epidermidisg+p+wgs, Staphylococcus equorumg, Staphylococcus haemolyticusg+wgs+gi, Staphylococcus hominiswgs, Staphylococcus pasteuriwgs, Staphylococcus pseudintermediusg+wgs+gi, Staphylococcus saprophyticusg+wgs+gi, Staphylococcus warnerig+wgs, Streptococcus agalactiaewgs, Streptococcus pyogenesg+wgs, Streptococcus suisg+wgs |
| Classification | 9 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic target replacement [Resistance Mechanism] + antibiotic target replacement protein + diaminopyrimidine antibiotic [Drug Class] + trimethoprim [Antibiotic] + antibiotic resistant dihydrofolate reductase |
| Parent Term(s) | 3 ontology terms | Show + trimethoprim resistant dihydrofolate reductase dfr [AMR Gene Family] + derives_from antibiotic sensitive dihydrofolate reductase + confers_resistance_to_antibiotic trimethoprim [Antibiotic] |
| Publications | Sekiguchi J, et al. 2005. Antimicrob Agents Chemother 49(9): 3948-3951. Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454). (PMID 16127079) |
Prevalence of dfrG among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Enterococcus avium | 0% | 0% | 8.77% | 0% |
| Enterococcus faecalis | 10.45% | 9.11% | 8.12% | 8.33% |
| Enterococcus faecium | 24.84% | 2.01% | 21.14% | 15.69% |
| Enterococcus hirae | 0% | 4.76% | 1.85% | 0% |
| Escherichia coli | 0% | 0% | 0.01% | 0% |
| Jeotgalibaca arthritidis | 0% | 0% | 0% | 33.33% |
| Jeotgalibaca porci | 0% | 0% | 0% | 50% |
| Klebsiella aerogenes | 0% | 0% | 0.28% | 0% |
| Klebsiella pneumoniae | 0% | 0% | 0.01% | 0% |
| Lactococcus garvieae | 0% | 4.17% | 0% | 0% |
| Ligilactobacillus animalis | 0% | 0% | 8.33% | 0% |
| Listeria innocua | 5.88% | 10.53% | 0% | 0% |
| Listeria monocytogenes | 0.28% | 0% | 0.02% | 0% |
| Staphylococcus arlettae | 16.67% | 0% | 7.5% | 0% |
| Staphylococcus aureus | 9.86% | 0.3% | 4.28% | 6.3% |
| Staphylococcus epidermidis | 3.87% | 0.29% | 4.94% | 0% |
| Staphylococcus equorum | 8.33% | 0% | 0% | 0% |
| Staphylococcus haemolyticus | 44.83% | 0% | 12.09% | 13.33% |
| Staphylococcus hominis | 0% | 0% | 0.98% | 0% |
| Staphylococcus pasteuri | 0% | 0% | 3.85% | 0% |
| Staphylococcus pseudintermedius | 36.67% | 0% | 43.59% | 6.67% |
| Staphylococcus saprophyticus | 11.76% | 0% | 4.2% | 100% |
| Staphylococcus warneri | 8.33% | 0% | 1.64% | 0% |
| Streptococcus agalactiae | 0% | 0% | 0.06% | 0% |
| Streptococcus pyogenes | 2.61% | 0% | 0.05% | 0% |
| Streptococcus suis | 0.8% | 0% | 1.15% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 300