vanL

Accession ARO:3002910
CARD Short NamevanL
DefinitionVanL is a D-Ala-D-Ala ligase homolog that can synthesize D-Ala-D-Ser, an alternative substrate for peptidoglycan synthesis that reduces vancomycin binding affinity in Enterococcus faecalis.
AMR Gene FamilyVan ligase, glycopeptide resistance gene cluster
Drug Classglycopeptide antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Sequence VariantsEnterococcus gilvuswgs
Classification13 ontology terms | Show
Parent Term(s)2 ontology terms | Show
Publications

Boyd DA, et al. 2008. Antimicrob Agents Chemother 52(7): 2667-2672. Molecular characterization of Enterococcus faecalis N06-0364 with low-level vancomycin resistance harboring a novel D-Ala-D-Ser gene cluster, vanL. (PMID 18458129)

Resistomes

Prevalence of vanL among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Enterococcus gilvus0%0%75%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 275


>gb|ABX54687.1|+|vanL [Enterococcus faecalis]
MMKLKKIAIIFGGQSSEYEVSLKSTVSVLETLSTCNFEIIKIGIDLGGKWYLTTSNNKDIEYDVWQTDPSLQEIIPCFNNRGFYNKTTNK
YFRPDVLFPILHGGTGEDGTLQGVFELMNIPYVGCGVTPSAICMDKYLLHEFAQSVGVKSAPTLIIRTRNCKDEIDKFIEKNDFPIFVKP
NEAGSSKGINKVNEPDKLEDALTEAFKYSKSVIIQKAIIGREIGCAVLGNEKLLVGECDEVSLNSDFFDYTEKYQMISAKVNIPASISVE
FSNEMKKQAQLLYRLLGCSGLARIDFFLSDNNEILLNEINTLPGFTEHSRYPKMMEAVGVTYKEIITKLINLAEEKYYG


>gb|EU250284.1|+|955-2004|vanL [Enterococcus faecalis]
ATGATGAAATTGAAAAAGATAGCCATAATATTCGGAGGTCAATCTTCGGAATATGAAGTCTCACTTAAATCAACAGTAAGTGTACTAGAA
ACTCTATCAACTTGTAATTTTGAAATTATAAAAATAGGAATTGATTTAGGCGGAAAGTGGTATCTCACCACAAGCAACAACAAAGATATT
GAATATGATGTTTGGCAAACTGATCCTTCATTACAAGAAATAATCCCATGTTTCAATAATCGAGGCTTTTATAACAAAACTACAAATAAA
TATTTCAGACCAGATGTACTCTTTCCAATTCTTCATGGGGGGACTGGAGAAGATGGAACCCTCCAAGGTGTATTTGAATTAATGAATATT
CCTTACGTTGGATGTGGGGTGACGCCTTCGGCTATTTGTATGGACAAATACTTATTGCATGAGTTTGCTCAGAGTGTGGGTGTAAAAAGT
GCCCCTACGCTCATAATTCGCACTAGAAACTGCAAAGATGAAATTGACAAGTTCATAGAAAAAAATGACTTCCCTATTTTTGTAAAGCCT
AACGAAGCGGGCTCATCAAAAGGAATAAACAAAGTAAATGAGCCAGATAAGCTAGAGGATGCTTTAACAGAAGCGTTTAAGTATAGTAAA
AGTGTTATCATTCAGAAAGCTATAATTGGAAGAGAAATTGGCTGTGCTGTCTTAGGTAATGAAAAACTCCTAGTAGGAGAATGTGATGAA
GTTTCCCTTAATAGCGATTTTTTTGATTATACCGAGAAATACCAAATGATCTCAGCAAAGGTAAATATACCTGCTTCTATATCTGTAGAA
TTTTCTAATGAAATGAAGAAACAAGCTCAGCTGTTATATAGGTTACTAGGCTGTTCAGGACTAGCACGAATTGATTTCTTCTTATCAGAT
AATAACGAAATACTATTAAACGAAATTAATACTTTGCCTGGTTTTACTGAGCATTCCAGATATCCCAAAATGATGGAAGCTGTAGGTGTT
ACCTATAAAGAGATTATCACGAAGTTAATCAATTTAGCGGAGGAAAAATATTATGGATAA