vanN

Accession ARO:3002912
CARD Short NamevanN
DefinitionVanN is a D-Ala-D-Ala ligase homolog that can synthesize D-Ala-D-Ser, an alternative substrate for peptidoglycan synthesis that reduces vancomycin binding affinity in Enterococcus faecium.
AMR Gene FamilyVan ligase, glycopeptide resistance gene cluster
Drug Classglycopeptide antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Perfect MatchesEnterococcus faeciump+wgs
Resistomes with Sequence VariantsEnterococcus aviumwgs, Enterococcus faeciump+wgs
Classification13 ontology terms | Show
Parent Term(s)2 ontology terms | Show
Publications

Lebreton F, et al. 2011. Antimicrob Agents Chemother 55(10): 4606-4612. D-Ala-d-Ser VanN-type transferable vancomycin resistance in Enterococcus faecium. (PMID 21807981)

Resistomes

Prevalence of vanN among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Enterococcus avium0%0%1.75%0%
Enterococcus faecium0%0.06%0.03%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 275


>gb|AEP40500.1|+|vanN [Enterococcus faecium]
MKKIALIFGGTSAEYEVSLKSAASVLSVLENLNVEIYRIGIASNGKWYLTFSDNETIANDLWLQDKKLNEITPSFDGRGFYDQAEKVYFK
PDVLFPMLHGGTGENGTLQGVFECMQIPYVGCGVASSAICMNKYLLHQFAKSVGVMSTPTQLISSTDEQQVIKNFTELYGFPIFIKPNEA
GSSKGISKVHTEAELTKALTEAFQFSQTVILQKAVSGVEIGCAILGNDQLLVGECDEVSLATDFFDYTEKYQMTTAKLTVPAKIPVATSR
EIKRQAQLLYQLLGCQGLARIDFFLTEAGEILLNEINTMPGFTNHSRFPAMMAATGITYQELISTLITLAEDK


>gb|JF802084.1|+|560-1591|vanN [Enterococcus faecium]
ATGAAAAAAATCGCCTTAATTTTTGGTGGTACTTCAGCAGAATATGAAGTATCCCTCAAATCAGCAGCTAGTGTTTTGTCTGTATTAGAA
AATCTAAATGTTGAAATTTACAGAATTGGCATAGCTTCGAACGGAAAATGGTATTTAACCTTTAGTGATAATGAAACTATTGCAAATGAC
TTATGGTTACAAGATAAAAAATTAAATGAGATCACTCCCTCCTTCGATGGGAGAGGGTTTTATGACCAAGCAGAAAAAGTATATTTTAAA
CCAGATGTCTTATTTCCGATGCTACACGGTGGCACTGGAGAAAATGGTACATTACAAGGAGTTTTTGAATGTATGCAAATTCCTTATGTT
GGTTGCGGCGTTGCCTCCTCTGCCATTTGTATGAATAAATATCTATTACATCAGTTTGCAAAAAGTGTCGGAGTGATGAGTACGCCTACA
CAGCTGATCTCATCGACGGACGAACAACAAGTAATCAAAAATTTTACTGAGTTGTACGGTTTTCCTATATTTATCAAACCAAATGAAGCT
GGTTCTTCAAAGGGAATCAGCAAAGTTCATACCGAAGCAGAGTTAACTAAAGCGCTGACCGAAGCGTTCCAATTCAGTCAGACAGTCATT
TTACAAAAAGCTGTTTCTGGAGTAGAGATCGGTTGCGCCATCCTAGGAAATGATCAATTGCTTGTTGGGGAATGTGATGAAGTATCCTTA
GCGACCGACTTTTTTGATTATACGGAAAAATATCAAATGACTACAGCAAAGTTGACCGTTCCAGCAAAAATTCCAGTGGCAACTAGTAGA
GAAATCAAGCGTCAAGCACAATTACTGTATCAATTACTTGGATGTCAGGGCTTAGCTCGCATTGATTTTTTTCTAACAGAAGCAGGTGAA
ATTCTCTTAAATGAAATCAATACAATGCCAGGCTTTACCAATCATTCTAGATTTCCAGCCATGATGGCAGCTACGGGTATCACTTATCAG
GAGCTTATTTCAACATTAATTACTTTAGCGGAGGATAAATAG