dfrA10

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Accession	ARO:3003011
CARD Short Name	dfrA10
Definition	dfrA10 is an integron-encoded dihydrofolate reductase found in Klebsiella pneumoniae.
AMR Gene Family	trimethoprim resistant dihydrofolate reductase dfr
Drug Class	diaminopyrimidine antibiotic
Resistance Mechanism	antibiotic target replacement
Resistomes with Perfect Matches	Acinetobacter baumannii^g, Acinetobacter pittii^wgs, Aeromonas veronii^g, Enterobacter hormaechei^wgs, Escherichia coli^wgs, Klebsiella oxytoca^wgs, Klebsiella pneumoniae^wgs, Morganella morganii^wgs, Proteus mirabilis^g+wgs, Providencia rettgeri^wgs, Pseudomonas aeruginosa^wgs, Salmonella enterica^wgs
Resistomes with Sequence Variants	Acinetobacter baumannii^g, Acinetobacter pittii^wgs, Aeromonas veronii^g, Enterobacter hormaechei^wgs, Escherichia coli^wgs, Klebsiella oxytoca^wgs, Klebsiella pneumoniae^wgs, Morganella morganii^wgs, Proteus mirabilis^g+wgs+gi, Providencia rettgeri^wgs, Pseudomonas aeruginosa^wgs, Salmonella enterica^wgs
Classification	9 ontology terms \| Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic target replacement [Resistance Mechanism] + antibiotic target replacement protein + diaminopyrimidine antibiotic [Drug Class] + antibiotic resistant dihydrofolate reductase + trimethoprim [Antibiotic]
Parent Term(s)	3 ontology terms \| Show + confers_resistance_to_antibiotic trimethoprim [Antibiotic] + derives_from antibiotic sensitive dihydrofolate reductase + trimethoprim resistant dihydrofolate reductase dfr [AMR Gene Family]
Publications	Partridge SR and Hall RM. 2002. Antimicrob Agents Chemother 47(1): 342-349. In34, a complex In5 family class 1 integron containing orf513 and dfrA10. (PMID 12499211)

Resistomes

Prevalence of dfrA10 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

Species	NCBI Chromosome	NCBI Plasmid	NCBI WGS	NCBI GI	GRDI-AMR2
Acinetobacter baumannii	0.18%	0%	0%	0%	0%
Acinetobacter baumannii	0.18%	0%	0%	0%	0%
Acinetobacter pittii	0%	0%	0.28%	0%	0%
Acinetobacter pittii	0%	0%	0.28%	0%	0%
Aeromonas veronii	1.82%	0%	0%	0%	0%
Aeromonas veronii	1.82%	0%	0%	0%	0%
Enterobacter hormaechei	0%	0%	0.04%	0%	0%
Enterobacter hormaechei	0%	0%	0.04%	0%	0%
Escherichia coli	0%	0%	0.02%	0%	0%
Escherichia coli	0%	0%	0.02%	0%	0%
Klebsiella oxytoca	0%	0%	0.42%	0%	0%
Klebsiella oxytoca	0%	0%	0.42%	0%	0%
Klebsiella pneumoniae	0%	0%	0.07%	0%	0%
Klebsiella pneumoniae	0%	0%	0.07%	0%	0%
Morganella morganii	0%	0%	0.61%	0%	0%
Morganella morganii	0%	0%	0.61%	0%	0%
Proteus mirabilis	3.67%	0%	0.5%	3.7%	0%
Proteus mirabilis	2.75%	0%	0.5%	0%	0%
Providencia rettgeri	0%	0%	13.38%	0%	0%
Providencia rettgeri	0%	0%	14.01%	0%	0%
Pseudomonas aeruginosa	0%	0%	0.4%	0%	0%
Pseudomonas aeruginosa	0%	0%	0.4%	0%	0%
Salmonella enterica	0%	0%	0.04%	0%	0%
Salmonella enterica	0%	0%	0.04%	0%	0%

Show Perfect Only

Detection Models

protein homolog model

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 300

Protein
DNA

>gb|AHG97174.1|+|dfrA10 [Klebsiella pneumoniae]
MNISLIFANELITRAFGNQGKLPWQFIKEDMQFFQKTTENSVVVMGLNTWRSLPKMKKLGRDFIVISSTITEHEVLNNNIQIFKSFESFL
EAFRDTTKPINVIGGVGLLSEAIEHASTVYMSSIHMVKPVHADVYVPVELMNKLYSDFKYPENILWVGDPIDSVYSLSIDKFVRPASLVG
VPNDINT

>gb|KF976462.2|+|111559-112122|dfrA10 [Klebsiella pneumoniae]
ATGAATATATCACTTATCTTTGCCAATGAATTAATTACCAGAGCATTCGGTAATCAAGGCAAATTACCTTGGCAATTCATTAAAGAAGAT
ATGCAGTTCTTCCAGAAGACTACAGAAAATTCTGTAGTCGTTATGGGATTAAATACATGGAGATCTCTACCTAAGATGAAGAAGCTTGGT
AGAGACTTCATTGTCATATCTTCAACTATCACAGAGCACGAAGTGCTCAACAATAATATCCAAATATTCAAATCATTTGAGAGCTTCTTA
GAAGCATTCAGAGACACAACCAAACCAATCAATGTCATTGGTGGTGTTGGTTTATTATCTGAAGCGATAGAACATGCTAGCACTGTTTAC
ATGAGTTCTATTCATATGGTTAAACCTGTTCATGCTGATGTGTATGTACCGGTAGAACTAATGAATAAACTCTATAGTGATTTCAAATAT
CCAGAAAATATTCTATGGGTAGGTGATCCAATAGATTCTGTGTATAGCTTGTCTATTGATAAGTTTGTTAGACCAGCTTCGCTGGTTGGG
GTGCCAAATGATATTAATACGTGA

Curator Acknowledgements

Curator	Description	Most Recent Edit

dfrA10

Prevalence: protein homolog model (view sequences)

Graph