dfrA25

Accession ARO:3003020
CARD Short NamedfrA25
DefinitiondfrA25 is an integron-encoded dihydrofolate reductase found in Salmonella agona.
AMR Gene Familytrimethoprim resistant dihydrofolate reductase dfr
Drug Classdiaminopyrimidine antibiotic
Resistance Mechanismantibiotic target replacement
Resistomes with Perfect MatchesEnterobacter hormaecheiwgs, Enterobacter kobeip, Escherichia colip+wgs, Klebsiella aerogenesp+wgs, Klebsiella michiganensisp, Klebsiella pneumoniaep+wgs, Klebsiella quasipneumoniaewgs, Proteus mirabiliswgs, Salmonella entericawgs
Resistomes with Sequence VariantsEnterobacter hormaecheiwgs, Enterobacter kobeip, Escherichia colip+wgs, Klebsiella aerogenesp+wgs, Klebsiella michiganensisp, Klebsiella pneumoniaep+wgs, Klebsiella quasipneumoniaewgs, Proteus mirabiliswgs, Salmonella entericawgs
Classification9 ontology terms | Show
Parent Term(s)3 ontology terms | Show
+ trimethoprim resistant dihydrofolate reductase dfr [AMR Gene Family]
+ derives_from antibiotic sensitive dihydrofolate reductase
+ confers_resistance_to_antibiotic trimethoprim [Antibiotic]
Publications

Agerso Y, et al. 2006. J Antimicrob Chemother 58(5): 1044-1047. dfrA25, a novel trimethoprim resistance gene from Salmonella Agona isolated from a human urine sample in Brazil. (PMID 16980698)

Resistomes

Prevalence of dfrA25 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Enterobacter hormaechei0%0%0.09%0%
Enterobacter kobei0%0.69%0%0%
Escherichia coli0%0.01%0.09%0%
Klebsiella aerogenes0%1.09%0.28%0%
Klebsiella michiganensis0%0.57%0%0%
Klebsiella pneumoniae0%0.09%0.26%0%
Klebsiella quasipneumoniae0%0%0.13%0%
Proteus mirabilis0%0%0.17%0%
Salmonella enterica0%0%0.09%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 300


>gb|ABB71176.1|+|dfrA25 [Salmonella enterica subsp. enterica serovar Agona]
MAARAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVGRKTFESMGPLPNRKYAVVTRSNWTAANENVVVFPSIDEAMGRLGEITDHVI
VAGGGEIYHETIPMASTLHVSTIDVEPEGDVFFPNIPGKFDVVFEQQFTSNINYCYQIWQKG


>gb|DQ267940.1|+|54-512|dfrA25 [Salmonella enterica subsp. enterica serovar Agona]
ATGGCTGCAAGAGCGAAAAATGGCGTAATCGGTTGCGGTCCTGACATTCCTTGGTCTGCCAAAGGGGAACAGCTTCTTTTCAAAGCACTG
ACCTATAACCAATGGCTTTTGGTAGGGCGCAAAACATTTGAGTCTATGGGGCCGCTGCCCAATAGGAAATACGCGGTTGTTACCCGCTCA
AACTGGACAGCGGCTAATGAAAACGTAGTGGTTTTCCCGTCGATTGACGAAGCGATGGGTAGATTAGGCGAGATCACTGACCATGTCATC
GTCGCCGGTGGTGGAGAAATCTACCATGAAACGATACCCATGGCCTCTACTCTGCATGTGTCGACAATCGACGTTGAGCCAGAGGGAGAC
GTTTTCTTTCCGAACATTCCTGGGAAGTTTGATGTCGTTTTTGAGCAACAATTTACATCAAACATTAACTATTGCTATCAAATCTGGCAA
AAGGGTTAA