mphL

Accession ARO:3003072
CARD Short NamemphL
DefinitionmphL is a chromosomally-encoded macrolide phosphotransferases that inactivate 14- and 15-membered macrolides such as erythromycin, clarithromycin, azithromycin.
AMR Gene Familymacrolide phosphotransferase (MPH)
Drug Classmacrolide antibiotic
Resistance Mechanismantibiotic inactivation
Resistomes with Perfect MatchesBacillus cereuswgs, Bacillus thuringiensiswgs
Resistomes with Sequence VariantsBacillus anthracisg+wgs, Bacillus cereusg+wgs, Bacillus thuringiensisg+wgs
Classification9 ontology terms | Show
Parent Term(s)4 ontology terms | Show
+ confers_resistance_to_antibiotic erythromycin [Antibiotic]
+ confers_resistance_to_antibiotic clarithromycin [Antibiotic]
+ confers_resistance_to_antibiotic azithromycin [Antibiotic]
+ macrolide phosphotransferase (MPH) [AMR Gene Family]
Publications

Wang C, et al. 2015. Environ Microbiol 17(5): 1560-1573. Functional characterization and phylogenetic analysis of acquired and intrinsic macrolide phosphotransferases in the Bacillus cereus group. (PMID 25059531)

Pawlowski AC, et al. 2018. Nat Commun 9(1):112 The evolution of substrate discrimination in macrolide antibiotic resistance enzymes. (PMID 29317655)

Resistomes

Prevalence of mphL among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 377 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Bacillus anthracis100%0%78.57%0%
Bacillus cereus40%0%7.56%0%
Bacillus thuringiensis36.25%0%17.1%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 500


>gb|EEL41021.1|-|mphL [Bacillus cereus Rock3-29]
MEEIKMNTLKIKQLATKEGLNILEDSIKINESGVDFQVAHAKEQNGDKWILRIPRRPESMRHVLQEKKALEIIKNHAGFQVPDWSIFTED
LIAYKQLSGVPAATIDIEQQGYIWSFNEKNAPSEYHISLGKVLANLHSLPQQEFNNIGIEILTANELRASMEQRMNRVKEQYYVNQKLWD
RWQAWLTEDSFWPSHVGVTHGDIHPGHILIDKKNNVTGLIDWTEVGIADVSIDFTSHYLLFGKDGLTKLISSYDNAGGKTWSRMDEHIIE
LLTTSSITVAEYAQVSGLKEMHEAAVHMLATES


>gb|ACMJ01000036.1|-|72075-72986|mphL [Bacillus cereus Rock3-29]
TTGGAGGAAATAAAAATGAACACACTTAAAATTAAACAATTAGCAACTAAGGAAGGCCTAAATATCTTAGAAGATTCAATAAAAATCAAT
GAATCTGGTGTTGACTTTCAAGTAGCACACGCTAAAGAACAAAACGGAGATAAATGGATACTAAGAATTCCTCGTAGACCAGAATCTATG
AGACATGTCCTACAAGAAAAAAAGGCATTGGAAATCATAAAAAACCATGCAGGATTCCAAGTTCCTGATTGGTCTATATTCACTGAAGAC
TTAATTGCCTATAAGCAACTAAGTGGCGTTCCTGCCGCCACTATTGATATAGAACAACAAGGATATATATGGAGCTTTAATGAAAAAAAC
GCACCATCTGAATACCATATTTCATTAGGAAAAGTTCTAGCGAATTTACACTCATTACCTCAACAAGAATTTAATAATATCGGTATTGAA
ATTCTTACTGCTAATGAATTAAGAGCTTCTATGGAACAAAGGATGAATCGAGTGAAGGAACAATACTATGTCAATCAAAAATTATGGGAT
CGTTGGCAAGCATGGCTAACTGAAGATTCTTTTTGGCCATCTCATGTAGGAGTAACGCATGGGGATATACATCCAGGTCATATCCTGATT
GATAAGAAAAATAATGTAACTGGCTTAATCGATTGGACAGAAGTAGGGATAGCTGATGTTTCTATAGATTTCACATCACATTATCTGCTC
TTTGGGAAAGATGGACTAACAAAGTTAATTAGCTCTTATGACAATGCTGGTGGTAAAACTTGGTCAAGAATGGATGAACATATTATCGAA
CTTCTAACAACAAGTAGTATCACTGTTGCTGAATATGCTCAAGTGTCAGGTTTGAAAGAGATGCATGAAGCAGCTGTACACATGCTAGCA
ACTGAAAGTTAA