| Accession | ARO:3003319 |
| CARD Short Name | Saur_mprF_DAP |
| Definition | Point mutations that occur within Staphylococcus aureus mprF gene resulting in resistance to daptomycin. |
| AMR Gene Family | daptomycin resistant mprF |
| Drug Class | peptide antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Classification | 13 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic molecule + antibiotic target alteration [Resistance Mechanism] + charge alteration conferring antibiotic resistance + peptide antibiotic [Drug Class] + determinant of antibiotic resistance + gene altering cell wall charge + mutation conferring antibiotic resistance + lipopeptide antibiotic + antibiotic resistant mprF + daptomycin [Antibiotic] + antibiotic resistant gene variant or mutant |
| Parent Term(s) | 2 ontology terms | Show + confers_resistance_to_antibiotic daptomycin [Antibiotic] + daptomycin resistant mprF [AMR Gene Family] |
| Publications | Yang SJ, et al. 2010. Antimicrob Agents Chemother 54(8): 3079-3085. Cell wall thickening is not a universal accompaniment of the daptomycin nonsusceptibility phenotype in Staphylococcus aureus: evidence for multiple resistance mechanisms. (PMID 20498310) Friedman L, et al. 2006. Antimicrob Agents Chemother 50(6): 2137-2145. Genetic changes that correlate with reduced susceptibility to daptomycin in Staphylococcus aureus. (PMID 16723576) Peleg AY, et al. 2012. PLoS One 7(1): E28316. Whole genome characterization of the mechanisms of daptomycin resistance in clinical and laboratory derived isolates of Staphylococcus aureus. (PMID 22238576) Bayer AS, et al. 2015. Antimicrob. Agents Chemother. 59(8):4930-7 Frequency and Distribution of Single-Nucleotide Polymorphisms within mprF in Methicillin-Resistant Staphylococcus aureus Clinical Isolates and Their Role in Cross-Resistance to Daptomycin and Host Defense Antimicrobial Peptides. (PMID 26055370) |
Prevalence of Staphylococcus aureus mprF with mutation conferring resistance to daptomycin among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI | GRDI-AMR2 |
|---|---|---|---|---|---|
| No prevalence data | |||||
Model Type: protein variant model
Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastP): 1600
PubMed: mutation data hand curated from the scientific literature, evaluated as conferring resistance (R). CRyPTIC: mutation data acquired from the CRyPTIC catalog, evaluated as resistant (R), susceptible (S), or undetermined (U). ReSeqTB: mutation data acquired from the ReSeqTB catalog, evaluated as conferring resistance (Minimal, Moderate, High), not conferring resistance (None), or Indeterminate. WHO: mutation data acquired from the WHO 2023 catalog, evaluated as resistant (R), susceptible (S), or undetermined (U).| Mutation | Mutation type | PubMed |
|---|---|---|
| G61V | single resistance variant | PMID:22238576 |
| S295L | single resistance variant | PMID:22238576 |
| P314L | single resistance variant | PMID:26055370 |
| S337L | single resistance variant | PMID:22238576 |
| L341S | single resistance variant | PMID:26055370 |
| T345A | single resistance variant | PMID:16723576 |
| T345I | single resistance variant | PMID:22238576 |
| T345K | single resistance variant | PMID:26055370 |
| M347R | single resistance variant | PMID:26055370 |
| V351E | single resistance variant | PMID:26055370 |
| I420N | single resistance variant | PMID:22238576 |
| T472K | single resistance variant | PMID:26055370 |
| L826F | single resistance variant | PMID:22238576 |
| Curator | Description | Most Recent Edit |
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