Escherichia coli EF-Tu mutants conferring resistance to kirromycin

Accession ARO:3003368
CARD Short NameEcol_EFTu_KIR
DefinitionSequence variants of Escherichia coli elongation factor Tu that confer resistance to kirromycin.
AMR Gene Familyelfamycin resistant EF-Tu
Drug Classelfamycin antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Sequence VariantsCronobacter sakazakiiwgs
Classification11 ontology terms | Show
Parent Term(s)3 ontology terms | Show
+ Escherichia coli EF-Tu mutants conferring resistance to elfamycin
+ derives_from elongation factor Tu
+ confers_resistance_to_antibiotic kirromycin [Antibiotic]
Publications

Hogg T, et al. 2002. Curr Protein Pept Sci 3(1): 121-131. Inhibitory mechanisms of antibiotics targeting elongation factor Tu. (PMID 12370016)

Mesters JR, et al. 1994. EMBO J 13(20): 4877-4885. The structural and functional basis for the kirromycin resistance of mutant EF-Tu species in Escherichia coli. (PMID 7525272)

Timms AR, et al. 1992. Mol. Gen. Genet. 232(1):89-96 Mutant sequences in the rpsL gene of Escherichia coli B/r: mechanistic implications for spontaneous and ultraviolet light mutagenesis. (PMID 1552908)

Resistomes

Prevalence of Escherichia coli EF-Tu mutants conferring resistance to kirromycin among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Cronobacter sakazakii0%0%0.22%0%
Show Perfect Only


Detection Models

Model Type: protein variant model

Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastP): 700

Legend:

  • discovered in clinical, agricultural, or environmental isolates

  • discovered via laboratory selection experiments

  • ReSeqTB https://platform.reseqtb.org

Published Variants:

PMID: 12370016L121Q Q125R Q125E Y161C Y161N Y161D G317D Q330H A376T A376S A376V E379K
PMID: 7525272Q125K

>gb|AAA50993.1|+|Escherichia coli EF-Tu mutants conferring resistance to kirromycin [Escherichia coli]
MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAARAFDQIDNAPEEKARG
ITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHI
LLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALE
GDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVG
EEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIK
PHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMV
VTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG



>gb|AH002539.2|+|1761-2945|Escherichia coli EF-Tu mutants conferring resistance to kirromycin [Escherichia coli]
GTGTCTAAAGAAAAATTTGAACGTACAAAACCGCACGTTAACGTTGGTACTATCGGCCACGTTGACCACGGTAAAACTACTCTGACCGCT
GCAATCACCACCGTACTGGCTAAAACCTACGGCGGTGCTGCTCGTGCATTCGACCAGATCGATAACGCGCCGGAAGAAAAAGCTCGTGGT
ATCACCATCAACACTTCTCACGTTGAATACGACACCCCGACCCGTCACTACGCACACGTAGACTGCCCGGGGCACGCCGACTATGTTAAA
AACATGATCACCGGTGCTGCTCAGATGGACGGCGCGATCCTGGTAGTTGCTGCGACTGACGGCCCGATGCCGCAGACTCGTGAGCACATC
CTGCTGGGTCGTCAGGTAGGCGTTCCGTACATCATCGTGTTCCTGAACAAATGCGACATGGTTGATGACGAAGAGCTGCTGGAACTGGTT
GAAATGGAAGTTCGTGAACTTCTGTCTCAGTACGACTTCCCGGGCGACGACACTCCGATCGTTCGTGGTTCTGCTCTGAAAGCGCTGGAA
GGCGACGCAGAGTGGGAAGCGAAAATCCTGGAACTGGCTGGCTTCCTGGATTCTTATATTCCGGAACCAGAGCGTGCGATTGACAAGCCG
TTCCTGCTGCCGATCGAAGACGTATTCTCCATCTCCGGTCGTGGTACCGTTGTTACCGGTCGTGTAGAACGCGGTATCATCAAAGTTGGT
GAAGAAGTTGAAATCGTTGGTATCAAAGAGACTCAGAAGTCTACCTGTACTGGCGTTGAAATGTTCCGCAAACTGCTGGACGAAGGCCGT
GCTGGTGAGAACGTAGGTGTTCTGCTGCGTGGTATCAAACGTGAAGAAATCGAACGTGGTCAGGTACTGGCTAAGCCGGGCACCATCAAG
CCGCACACCAAGTTCGAATCTGAAGTGTACATTCTGTCCAAAGATGAAGGCGGCCGTCATACTCCGTTCTTCAAAGGCTACCGTCCGCAG
TTCTACTTCCGTACTACTGACGTGACTGGTACCATCGAACTGCCGGAAGGCGTAGAGATGGTAATGCCGGGCGACAACATCAAAATGGTT
GTTACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTTGCT
AAAGTTCTGGGCTAA