mecB

Ontology CARD's Antibiotic Resistance Ontology
Accession ARO:3003440
Synonym(s)PBP2B
CARD Short NamemecB
DefinitionmecB encodes an alternative PBP2b protein involved in bacterial cell wall formation. These proteins are acquired by lateral gene transfer and commonly associated with methicillin-resistant Staphylococcus aureus (MRSA). These proteins have lower affinity for penicillin-like antibiotics and therefore are able to perform peptidoglycan synthesis in the presence of beta-lactams.
AMR Gene FamilyPBP2-like beta-lactam resistant peptidoglycan transpeptidase
Drug Classpenicillin beta-lactam, cephalosporin, carbapenem, monobactam
Resistance Mechanismantibiotic target replacement
CARD:Epi text mining of PubMed up to 2021

(top ten associations if supported by more than one publication)
NCBI_TAXONStreptococcus pneumoniae (17), Staphylococcus aureus (4), Macrococcus (3), Macrococcus canis (3), Streptococcus mitis (2), Macrococcus goetzii (2), Macrococcus caseolyticus (2), Escherichia coli (2), Streptococcus (2)
GAZ
FOODON
ENVO
UBERON
Classification71 ontology terms | Show
+ process or component of antibiotic biology or chemistry
+ antibiotic molecule
+ beta-lactam antibiotic
+ penicillin beta-lactam [Drug Class]
+ cephalosporin [Drug Class]
+ beta-lactamase resistant penicillin
+ beta-lactamase sensitive penicillin
+ penicillin with extended spectrum
+ other cephalosporins and penems
+ fourth-generation cephalosporin [Drug Subclass]
+ third-generation cephalosporin [Drug Subclass]
+ second-generation cephalosporin [Drug Subclass]
+ first-generation cephalosporin [Drug Subclass]
+ antibiotic target
+ carbapenem [Drug Class]
+ monobactam [Drug Class]
+ moxalactam [Antibiotic]
+ loracarbef [Antibiotic]
+ cefradine [Antibiotic]
+ cephapirin [Antibiotic]
+ ceftizoxime [Antibiotic]
+ ceftiofur [Antibiotic]
+ cefprozil [Antibiotic]
+ cefonicid [Antibiotic]
+ cefmetazole [Antibiotic]
+ mecillinam [Antibiotic]
+ cell wall synthesis enzyme targeted by antibiotic
+ cefalotin [Antibiotic]
+ ceftaroline [Antibiotic]
+ cefdinir [Antibiotic]
+ cefditoren [Antibiotic]
+ ceftibuten [Antibiotic]
+ cefpodoxime [Antibiotic]
+ cefixime [Antibiotic]
+ cefotaxime [Antibiotic]
+ cefaclor [Antibiotic]
+ cefotiam [Antibiotic]
+ cefadroxil [Antibiotic]
+ cefalexin [Antibiotic]
+ doripenem [Antibiotic]
+ mezlocillin [Antibiotic]
+ azlocillin [Antibiotic]
+ ampicillin [Antibiotic]
+ flucloxacillin [Antibiotic]
+ dicloxacillin [Antibiotic]
+ propicillin [Antibiotic]
+ phenoxymethylpenicillin [Antibiotic]
+ benzylpenicillin [Antibiotic]
+ aztreonam [Antibiotic]
+ imipenem [Antibiotic]
+ mechanism of antibiotic resistance
+ piperacillin [Antibiotic]
+ meropenem [Antibiotic]
+ ertapenem [Antibiotic]
+ amoxicillin [Antibiotic]
+ cefuroxime [Antibiotic]
+ ceftriaxone [Antibiotic]
+ ceftobiprole [Antibiotic]
+ ceftazidime [Antibiotic]
+ cefepime [Antibiotic]
+ cefazolin [Antibiotic]
+ oxacillin [Antibiotic]
+ carbenicillin [Antibiotic]
+ methicillin [Antibiotic]
+ cloxacillin [Antibiotic]
+ cefoxitin [Antibiotic]
+ beta-lactam sensitive penicillin-binding protein
+ determinant of antibiotic resistance
+ antibiotic target replacement [Resistance Mechanism]
+ beta-lactam resistant penicillin-binding proteins
+ antibiotic target replacement protein
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic methicillin [Antibiotic]
+ PBP2-like beta-lactam resistant peptidoglycan transpeptidase [AMR Gene Family]
Resistomes with Sequence VariantsMacrococcoides canisg+gi
Publications

Harrison EM, et al. 2014. J Antimicrob Chemother 69(4): 911-918. A novel hybrid SCCmec-mecC region in Staphylococcus sciuri. (PMID 24302651)

Resistomes

Prevalence of mecB among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GIGRDI-AMR2
Macrococcoides canis50%0%0%100%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 1300


>gb|WP_012655867.1|+|mecB [Macrococcus caseolyticus]
MKNKALAILIICICLLIAYNFVKKDEVDKIFDAIELRDSEYLNEHATFLSKSLYDKDQRYKRMDKIDASLGIKEVKVSNVRLVQKKKNKR
QYSANLNFRTKYGNFSREGNYSFEKDEITKKWLLDWSPEVIIPGLTDRNQISIETLESFRGKILDRNGIDIAKDGIHYEVGIDIKNLNKK
NKKNISKLLSISESTLNKKLKQTWVKEGVFLPLKSYIELDDELKLGIQKYHLTVNQTKGRVYPLREATVHLLGYVGEINAEELKNKKFKD
YDEHSIVGKSGIELQYDKQLQNKDGYKVVITSDDALNNDEDVLLEKKPKNGQDIILTIDSKVQRSIYSHLKEDNGSGVAMNPKTGELLAL
VSYPAYDPYEFMFGISDENYKKIVNDKKEPLLNKFQTTSSPGSTQKLITSIIGLKNGTIDASTSYNIVTKGWQRNSSWGGYEVTRFEEVN
GDIDLEKAIAHSDNIFFARATLDMGSEKFIKGMKALDIGRNIPSDYYFQKGQIANPESLKNNFKNEILLADSGYGQGEILISPVQILSIY
SSLINEGKMMKPKLFETTKEDIWKNHIISKDDVDILTRSMRKVVTGTHRLDAERNYAQFAGKTGTAELKTSREEGLGAQIGWFVGYDQNN
PNMMLGISVKNVENKGMSSYNARKFAEIMDELYENGTKKYEIDR


>gb|NG_047953.1|+|101-2125|mecB [Macrococcus caseolyticus]
ATGAAAAATAAAGCTTTAGCAATTTTAATTATATGTATTTGCCTTTTAATAGCATATAATTTTGTGAAAAAAGATGAAGTTGATAAAATA
TTTGATGCTATTGAATTAAGGGATTCGGAATATCTGAATGAACATGCGACATTCTTATCTAAAAGTCTTTACGACAAAGATCAGAGATAT
AAAAGAATGGATAAGATTGATGCTTCTCTTGGTATTAAAGAAGTGAAAGTTAGTAATGTACGACTCGTGCAAAAGAAAAAAAATAAACGT
CAATATAGTGCAAATTTAAATTTTAGAACTAAATATGGTAATTTTTCTAGAGAAGGGAACTATTCTTTTGAAAAGGATGAAATAACAAAA
AAATGGCTTTTGGATTGGTCACCTGAGGTTATAATACCGGGATTGACTGATAGAAATCAAATCAGTATAGAAACCTTGGAATCTTTCAGA
GGGAAAATACTAGACAGAAACGGGATTGATATAGCGAAAGACGGAATTCATTACGAAGTTGGAATAGATATTAAAAATTTAAATAAAAAA
AATAAGAAAAATATTTCAAAATTGTTATCAATAAGTGAATCGACACTAAATAAAAAGTTAAAACAAACATGGGTAAAAGAAGGTGTTTTT
TTACCTTTAAAATCGTACATAGAGTTGGATGATGAACTTAAATTGGGTATCCAAAAATATCATTTGACGGTTAATCAAACAAAAGGTAGG
GTTTATCCATTAAGAGAAGCAACAGTACATCTTTTAGGGTATGTTGGAGAAATTAATGCTGAAGAATTAAAGAATAAAAAGTTTAAGGAT
TATGATGAACACTCAATCGTAGGAAAAAGTGGTATCGAACTACAATATGATAAACAATTGCAAAATAAAGATGGTTATAAAGTTGTCATA
ACTAGTGATGATGCATTAAATAATGATGAAGATGTCTTGTTAGAAAAGAAACCAAAAAATGGACAGGACATTATATTAACAATTGATAGC
AAAGTACAAAGAAGTATATATAGTCATTTAAAAGAAGATAATGGTTCAGGAGTAGCCATGAATCCTAAAACTGGTGAATTATTAGCTTTA
GTTAGTTATCCTGCATATGACCCCTATGAGTTTATGTTCGGCATTTCCGACGAAAACTACAAAAAGATAGTTAATGATAAGAAAGAGCCC
CTGTTAAATAAATTTCAGACAACCTCTTCCCCAGGATCTACTCAGAAATTAATAACATCTATCATAGGTTTGAAAAATGGGACTATAGAC
GCATCAACCAGCTACAACATAGTAACTAAGGGATGGCAGAGAAATTCTTCATGGGGAGGATATGAAGTTACAAGGTTTGAGGAAGTTAAT
GGAGATATTGATTTAGAAAAAGCGATAGCACATTCTGATAATATATTTTTTGCAAGAGCTACCCTCGATATGGGTTCCGAAAAATTTATT
AAGGGCATGAAAGCTTTAGACATTGGGAGAAATATTCCTTCTGATTATTATTTTCAAAAAGGACAAATTGCAAATCCAGAAAGTTTAAAA
AATAATTTTAAAAATGAAATATTACTAGCTGATTCAGGATATGGCCAGGGAGAAATACTTATAAGTCCAGTACAAATATTATCTATTTAT
AGTTCTTTAATTAATGAAGGTAAAATGATGAAACCTAAATTATTTGAAACAACAAAAGAAGATATTTGGAAAAATCATATTATTTCAAAA
GATGACGTAGATATATTAACAAGAAGCATGAGAAAAGTAGTTACTGGGACACATAGATTGGATGCAGAAAGAAATTATGCACAGTTTGCT
GGAAAAACTGGCACTGCAGAATTGAAAACCTCTAGAGAAGAGGGGTTAGGAGCTCAAATCGGTTGGTTTGTTGGATATGATCAAAATAAT
CCCAATATGATGTTAGGTATAAGTGTGAAGAATGTAGAGAATAAAGGTATGTCGAGTTATAATGCAAGAAAGTTTGCCGAGATAATGGAT
GAATTATATGAAAATGGAACGAAAAAATATGAAATAGATAGGTGA

Curator Acknowledgements
Curator Description Most Recent Edit