Pseudomonas aeruginosa parE conferring resistance to fluoroquinolones

Accession ARO:3003685
CARD Short NamePaer_parE_FLO
DefinitionPoint mutation in Pseudomonas aeruginosa parE resulting in sensitivity to fluoroquinolones (ciprofloxacin). In combination with a gyrase mutation (gyrA or gyrB), it confers a high level of resistance to ciprofloxacin.
AMR Gene Familyfluoroquinolone resistant parE
Drug Classfluoroquinolone antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Sequence VariantsPseudomonas aeruginosag+wgs
Classification11 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ fluoroquinolone resistant parE [AMR Gene Family]
+ confers_resistance_to_antibiotic ciprofloxacin [Antibiotic]
Publications

Bruchmann S, et al. 2013. Antimicrob. Agents Chemother. 57(3):1361-8 Quantitative contributions of target alteration and decreased drug accumulation to Pseudomonas aeruginosa fluoroquinolone resistance. (PMID 23274661)

Resistomes

Prevalence of Pseudomonas aeruginosa parE conferring resistance to fluoroquinolones among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 377 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Pseudomonas aeruginosa3.94%0%1.65%0%
Show Perfect Only


Detection Models

Model Type: protein variant model

Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastP): 1000

Legend:

  • discovered in clinical, agricultural, or environmental isolates

  • discovered via laboratory selection experiments

  • ReSeqTB https://platform.reseqtb.org

Published Variants:

PMID: 23274661M437I A473V

>gb|AAG08352.1|-|Pseudomonas aeruginosa parE conferring resistance to fluoroquinolones [Pseudomonas aeruginosa PAO1]
MATYNADAIEVLSGLDPVRKRPGMYTDTTRPNHLAQEVIDNSVDEALAGHAKSVQVILHQ
DNSLEVIDDGRGMPVDIHPEEGVPGVELILTKLHAGGKFSNKNYQFSGGLHGVGISVVNA
LSTRVEVRVKRDANEYRMTFADGFKDSDLEVIGTVGKRNTGTSVHFWPDPKYFDSAKFSV
SRLKHVLKAKAVLCPGLSVVFEDKNTGERVEWHFEDGLRSYLTDAVAELPRLPDEPFCGN
LEGSKEAVSWALLWLPEGGESVQESYVNLIPTAQGGTHVNGLRQGLLDAMREFCEFRNLL
PRGVKLAPEDVWERIAFVLSMKMQEPQFSGQTKERLSSREAAAFVSGVVKDAFSLWLNEH
AEIGLQLAELAISNAGRRLKAGKKVERKKITQGPALPGKLADCAGQEPMRAELFLVEGDS
AGGSAKQARDKEFQAIMPLRGKILNTWEVDGGEVLASQEVHDIAVAIGVDPGASDLAQLR
YGKICILADADSDGLHIATLLCALFVRHFRPLVEAGHVYVAMPPLYRIDLGKDIYYALDE
AERDGILERLAAEKKRGKPQVTRFKGLGEMNPLQLRETTMDPNTRRLVQLTLEDATGTLE
IMDMLLAKKRAGDRKSWLESKGNLAEVLV



>gb|AE004091.2|-|5576028-5577917|Pseudomonas aeruginosa parE conferring resistance to fluoroquinolones [Pseudomonas aeruginosa PAO1]
ATGGCTACTTACAACGCAGACGCCATCGAAGTCCTTTCCGGCCTCGACCCGGTGCGCAAGCGCCCGGGGATGTACACCGACACCACCCGC
CCCAACCATCTGGCCCAGGAAGTCATCGACAACAGCGTCGACGAAGCCCTGGCCGGCCATGCGAAGAGCGTGCAGGTGATCCTGCACCAG
GACAACTCGCTGGAAGTCATCGACGATGGCCGCGGCATGCCGGTGGACATCCACCCGGAAGAGGGCGTGCCGGGCGTCGAGCTGATCCTT
ACCAAGCTGCATGCCGGCGGCAAGTTCTCGAACAAGAACTACCAGTTCTCCGGCGGCTTGCACGGGGTCGGCATCTCGGTGGTGAACGCG
CTCTCGACCCGGGTCGAGGTACGCGTCAAGCGCGACGCCAACGAGTACCGGATGACCTTCGCCGACGGCTTCAAGGACAGCGATCTGGAA
GTCATCGGCACGGTCGGCAAGCGCAATACCGGTACCAGCGTGCATTTCTGGCCGGATCCGAAGTATTTCGATTCGGCGAAGTTCTCGGTC
AGCCGCCTCAAGCATGTGCTCAAGGCCAAGGCGGTGCTGTGCCCGGGCCTGAGCGTGGTGTTCGAGGACAAGAACACCGGCGAGCGCGTC
GAGTGGCACTTCGAGGACGGCCTGCGCTCCTACCTGACCGACGCGGTCGCCGAGCTGCCGCGCCTGCCCGATGAACCCTTCTGCGGCAAC
CTCGAAGGTTCCAAGGAAGCGGTGAGCTGGGCCCTGCTGTGGCTGCCCGAGGGCGGTGAGTCGGTGCAGGAAAGCTACGTCAACCTGATT
CCCACGGCCCAGGGCGGCACCCATGTGAACGGCCTGCGCCAGGGCCTGCTCGACGCCATGCGCGAGTTCTGCGAGTTCCGCAACCTGTTG
CCGCGCGGCGTCAAGCTGGCGCCCGAGGACGTCTGGGAGCGGATCGCCTTCGTCCTCTCGATGAAGATGCAGGAGCCGCAGTTCTCCGGG
CAGACCAAGGAGCGCCTGTCGTCCCGCGAGGCGGCGGCGTTCGTCTCGGGCGTGGTGAAGGACGCCTTCAGCCTGTGGCTCAACGAGCAC
GCCGAAATCGGCCTGCAACTGGCGGAACTGGCGATCAGCAACGCCGGGCGTCGCCTCAAGGCGGGCAAGAAGGTCGAGCGCAAGAAGATC
ACCCAGGGGCCGGCGCTGCCCGGCAAACTGGCCGACTGCGCCGGACAGGAACCGATGCGCGCGGAACTGTTCCTGGTCGAGGGCGACTCC
GCCGGCGGCTCGGCGAAGCAGGCGCGGGACAAGGAATTCCAGGCGATCATGCCGCTGCGCGGAAAGATCCTGAACACCTGGGAAGTGGAC
GGCGGCGAGGTGCTCGCCAGCCAGGAGGTCCACGACATCGCGGTGGCCATCGGCGTCGATCCGGGTGCCAGTGACCTGGCCCAGCTGCGC
TACGGCAAGATCTGTATCCTCGCGGATGCCGACTCCGACGGGCTGCACATCGCCACGCTGCTCTGCGCGCTGTTCGTCCGCCATTTCCGC
CCGCTGGTGGAAGCCGGCCACGTCTACGTGGCGATGCCGCCGCTGTACCGCATCGACCTCGGCAAGGACATCTACTACGCCCTCGACGAA
GCCGAGCGCGACGGCATCCTCGAGCGCCTGGCCGCAGAGAAGAAGCGCGGCAAGCCGCAGGTCACCCGCTTCAAGGGCCTTGGCGAAATG
AATCCGTTGCAACTGCGCGAGACCACCATGGATCCGAATACCCGGCGGCTGGTCCAGCTCACCCTGGAGGACGCCACCGGTACCCTGGAG
ATCATGGACATGCTGCTGGCCAAGAAGCGCGCCGGTGACCGCAAGTCCTGGCTGGAAAGCAAGGGCAACCTGGCCGAGGTGCTGGTCTGA