PmpM

Accession ARO:3004077
Synonym(s)PA1361
CARD Short NamePmpM
DefinitionPmpM is a multidrug efflux pump belonging to the MATE family of Pseudomonas aeruginosa. PmpM is an H+ drug antiporter and is the first reported case of an H+ coupled efflux pump in the MATE family. PmpM confers resistance to fluoroquinolones, fradiomycin, benzalkonium chloride, chlorhexidine gluconate, ethidium bromide, tetraphenylphosphonium chloride (TPPCl), and rhodamine 6G.
AMR Gene Familymultidrug and toxic compound extrusion (MATE) transporter
Drug Classdisinfecting agents and antiseptics, aminoglycoside antibiotic, fluoroquinolone antibiotic
Resistance Mechanismantibiotic efflux
Efflux Componentefflux pump complex or subunit conferring antibiotic resistance
Resistomes with Perfect MatchesPseudomonas aeruginosag+wgs, Pseudomonas fluorescensg
Resistomes with Sequence VariantsPseudomonas aeruginosag+wgs, Pseudomonas fluorescensg
Classification8 ontology terms | Show
Parent Term(s)5 ontology terms | Show
+ multidrug and toxic compound extrusion (MATE) transporter [AMR Gene Family]
+ confers_resistance_to_drug_class fluoroquinolone antibiotic [Drug Class]
+ confers_resistance_to_antibiotic neomycin [Antibiotic]
+ confers_resistance_to_antibiotic benzalkonium chloride [Antibiotic]
+ confers_resistance_to_antibiotic chlorhexidine [Antibiotic]
Publications

He GX, et al. 2004. J. Bacteriol. 186(1):262-5 An H(+)-coupled multidrug efflux pump, PmpM, a member of the MATE family of transporters, from Pseudomonas aeruginosa. (PMID 14679249)

Resistomes

Prevalence of PmpM among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Pseudomonas aeruginosa98.77%0%66.85%0%
Pseudomonas fluorescens2.78%0%0%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 900


>gb|AAG04750.1|-|PmpM [Pseudomonas aeruginosa PAO1]
MNSPALPLSRGLRIRAELKELLTLAAPIMIAQLATTAMGFVDAVMAGRASPHDLAAVALGNSIWIPMFLLMTGTLLATTAKVAQRHGAGD
QPGTGPLVRQALWLALLIGPLSGAVLWWLSEPILGLMKVRPELIGPSLLYLKGIALGFPAAALYHVLRCYTNGLGRTRPSMVLGIGGLLL
NIPINYALIYGHFGMPKMGGPGCGWATGSVMWFMFLGMLFWVNKASIYRASQLFSRWEWPDRATIGPLVAVGLPIGIAVFAESSIFSVIA
LLIGGLDENVVAGHQIALNFSALVFMIPYSLGMAVTVRVGHNLGAGLPRDARFAAGVGMAAALGYACVSASLMLLLREQIAAMYSPDPAV
IAIAASLIVFSALFQFSDALQVTAAGALRGYQDTRVTMIMTLFAYWGIGLPVGYSLGLTDWFQEPTGPRGLWQGLVVGLTGAAIMLCIRL
ARSARRFIRQHERLQREDAEAASVLGR


>gb|AE004091.2|-|1472547-1473980|PmpM [Pseudomonas aeruginosa PAO1]
GTGAACAGCCCCGCCCTGCCCCTTTCCCGTGGCTTGCGCATCCGCGCCGAACTCAAGGAACTGCTGACCCTCGCCGCGCCGATCATGATC
GCGCAACTGGCGACCACCGCCATGGGCTTCGTCGATGCGGTGATGGCCGGGCGCGCCAGTCCGCACGACCTGGCAGCGGTGGCGCTGGGC
AACTCCATCTGGATCCCGATGTTCCTGCTGATGACCGGCACCCTGCTCGCCACCACGGCCAAGGTCGCCCAGCGCCATGGCGCCGGCGAC
CAGCCCGGCACCGGGCCGCTGGTGCGCCAGGCGCTGTGGCTGGCGCTGCTGATCGGACCGCTGTCGGGGGCGGTGCTGTGGTGGTTGTCG
GAGCCGATCCTCGGCTTGATGAAAGTGCGCCCGGAACTGATCGGGCCGAGCCTGCTGTACCTCAAGGGCATCGCCCTGGGCTTCCCGGCG
GCGGCGCTGTACCACGTACTGCGCTGCTACACCAACGGCCTGGGACGGACCCGGCCGAGCATGGTGCTGGGGATCGGCGGGCTGCTGCTG
AACATCCCGATCAACTACGCGCTGATCTACGGCCACTTCGGCATGCCGAAGATGGGTGGCCCCGGCTGCGGCTGGGCCACCGGCTCGGTG
ATGTGGTTCATGTTCCTCGGCATGCTGTTCTGGGTGAACAAGGCCTCGATCTACCGCGCCAGCCAGTTGTTCTCGCGCTGGGAGTGGCCG
GATCGCGCGACCATCGGCCCGCTGGTGGCGGTCGGCCTGCCGATCGGCATCGCGGTGTTCGCCGAGTCGAGCATCTTCTCGGTGATCGCC
CTGCTGATCGGCGGGCTCGACGAGAACGTGGTGGCCGGCCACCAGATCGCCCTGAACTTCAGCGCGCTGGTGTTCATGATTCCCTATTCG
CTGGGGATGGCGGTGACCGTGCGGGTCGGCCACAACCTCGGCGCCGGCCTGCCGCGCGACGCGCGCTTCGCCGCCGGCGTGGGGATGGCC
GCGGCGCTGGGCTACGCCTGCGTCTCGGCGAGCCTGATGTTGTTGCTGCGCGAGCAGATCGCCGCGATGTATTCGCCGGACCCGGCGGTG
ATCGCCATCGCCGCCTCGCTGATCGTGTTCTCCGCGCTGTTCCAGTTCTCCGACGCCCTGCAGGTCACCGCCGCCGGGGCCCTGCGCGGC
TACCAGGACACCCGGGTGACGATGATCATGACCCTGTTCGCCTACTGGGGCATCGGCCTGCCGGTGGGCTACAGCCTCGGCCTCACCGAC
TGGTTCCAGGAACCCACCGGACCGCGCGGTCTGTGGCAAGGCCTGGTGGTGGGCCTGACCGGCGCGGCGATCATGCTCTGCATCCGCCTG
GCGCGCAGCGCGCGGCGCTTCATCCGCCAGCACGAGCGCCTGCAGCGGGAGGACGCGGAGGCCGCCTCAGTCCTTGGCCGGTAG