APH(3')-IXa

Accession ARO:3004087
CARD Short NameAPH(3')-IXa
DefinitionAPH(3')-IXa is an aminoglycoside phosphoryltransferase that acts on the 3-OH target of aminoglycosides.
AMR Gene FamilyAPH(3')
Drug Classaminoglycoside antibiotic
Resistance Mechanismantibiotic inactivation
Classification10 ontology terms | Show
Parent Term(s)3 ontology terms | Show
+ APH(3') [AMR Gene Family]
+ confers_resistance_to_antibiotic kanamycin A [Antibiotic]
+ confers_resistance_to_antibiotic amikacin [Antibiotic]
Publications

Yoon EJ, et al. 2017. J. Antibiot. 70(4):400-403 New aminoglycoside-modifying enzymes APH(3')-VIII and APH(3')-IX in Acinetobacter rudis and Acinetobacter gerneri. (PMID 27965516)

Resistomes

Prevalence of APH(3')-IXa among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 377 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
No prevalence data


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 300


>gb|ENV34035.1|+|APH(3')-IXa [Acinetobacter gerneri DSM 14967 = CIP 107464 = MTCC 9824]
MINDMKISLPQSLKSFIGNQPLQKDKVGQSPSDVYSFTKNNEKYYLKTTELIYAQTTYSIIREAKILDWLDGKLNVPELVLMDTDHENEY
MISKAVPAKPLQDFTGKSDQFFIDIYTDALAQLQSISIKNCPFISNKKFRLAEAEFFIENGLLDELDDDEKDLKLWSSYQNFAEFLDDLK
QQNFQEEYVFSHGDLTDSNVFLSHDAQIYFLDVGRAGIADRFVDIAFIERSLREDCSEDAALQFLNHLAEDDSFKRNYFLKLDELN


>gb|APPN01000061.1|+|80498-81298|APH(3')-IXa [Acinetobacter gerneri DSM 14967 = CIP 107464 = MTCC 9824]
ATGATCAATGATATGAAAATTTCACTTCCGCAAAGTCTTAAAAGTTTTATTGGAAATCAACCATTACAAAAAGATAAAGTGGGACAATCC
CCTTCTGATGTGTATTCTTTTACCAAAAATAATGAAAAATACTATTTAAAAACCACTGAGCTAATTTATGCTCAAACAACTTATAGTATC
ATACGTGAAGCAAAAATACTCGACTGGCTAGATGGAAAGCTCAATGTTCCAGAGCTAGTTTTAATGGATACCGACCATGAAAATGAGTAC
ATGATCAGCAAGGCAGTCCCCGCAAAACCCTTACAAGATTTCACAGGAAAAAGTGATCAATTTTTCATAGATATTTATACAGATGCTTTG
GCACAATTACAGTCTATTTCAATAAAAAACTGTCCATTTATATCCAATAAAAAATTTCGATTAGCTGAGGCGGAATTCTTTATTGAAAAT
GGCTTACTTGATGAATTGGATGATGATGAAAAAGATCTAAAGCTTTGGTCCAGTTATCAAAATTTTGCTGAATTCTTAGATGATCTAAAA
CAGCAAAATTTCCAAGAAGAATATGTATTTAGTCATGGTGACCTAACCGACAGCAATGTATTTTTAAGTCATGATGCACAAATCTACTTT
TTAGATGTCGGACGTGCTGGTATCGCAGATCGATTTGTAGATATTGCCTTTATTGAACGTAGTCTACGAGAAGATTGCTCTGAGGATGCT
GCACTTCAATTTTTAAATCATTTAGCAGAAGACGATTCTTTTAAACGGAATTATTTTCTAAAATTGGATGAGTTAAATTGA