Chlamydia trachomatis 23S rRNA with mutation conferring resistance to macrolide antibiotics

Accession ARO:3004174
CARD Short NameCtra_23S_MAC
DefinitionPoint mutation in the 23S rRNA of Chlamydia trachomatis shown to confer resistance to macrolide type antibiotics.
AMR Gene Family23S rRNA with mutation conferring resistance to macrolide antibiotics
Drug Classmacrolide antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Sequence VariantsChlamydia psittaciwgs, Chlamydia suisg+wgs, Chlamydia trachomatisg+wgs
Classification10 ontology terms | Show
Parent Term(s)4 ontology terms | Show
+ confers_resistance_to_antibiotic erythromycin [Antibiotic]
+ confers_resistance_to_antibiotic azithromycin [Antibiotic]
+ confers_resistance_to_antibiotic josamycin [Antibiotic]
+ 23S rRNA with mutation conferring resistance to macrolide antibiotics [AMR Gene Family]
Publications

Misyurina OY, et al. 2004. Antimicrob. Agents Chemother. 48(4):1347-9 Mutations in a 23S rRNA gene of Chlamydia trachomatis associated with resistance to macrolides. (PMID 15047540)

Jiang Y, et al. 2015. Exp Ther Med 10(3):1189-1193 Differences in 23S ribosomal RNA mutations between wild-type and mutant macrolide-resistant isolates. (PMID 26622462)

Binet R and Maurelli AT. 2007. Antimicrob Agents Chemother 51(12): 4267-4275. Frequency of development and associated physiological cost of azithromycin resistance in Chlamydia psittaci 6BC and C. trachomatis L2. (PMID 17908942)

Resistomes

Prevalence of Chlamydia trachomatis 23S rRNA with mutation conferring resistance to macrolide antibiotics among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 377 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: rRNA gene variant model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Chlamydia psittaci0%0%1.92%0%
Chlamydia suis87.5%0%100%0%
Chlamydia trachomatis99.25%0%92.11%0%
Escherichia coli0%0%0%0%
Show Perfect Only


Detection Models

Model Type: rRNA gene variant model

Model Definition: Ribosomal RNA (rRNA) Gene Variant Models (RVM) are similar to Protein Variant Models (PVM), i.e. detect sequences based on their similarity to a curated reference sequence and secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles, except RVMs are designed to detect AMR acquired via mutation of genes encoding ribosomal RNAs (rRNA). RVMs include a rRNA reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTN bit-score above the curated BLASTN cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTN bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastN): 5000

Legend:

  • discovered in clinical, agricultural, or environmental isolates

  • discovered via laboratory selection experiments

  • ReSeqTB https://platform.reseqtb.org

Published Variants:

PMID: 15047540A2107C A2663C
PMID: 26622462A2107G A2109G T2663C
PMID: 17908942C196A


>gb|NR_076160.1|+|1-2940|Chlamydia trachomatis 23S rRNA with mutation conferring resistance to macrolide antibiotics [Chlamydia trachomatis]
CAGACCAAGTTAATAAGAGCTATTGGTGGATGCCTTGGCATTGACAGGCGAAGAAGGACGCGAATACCTGCGAAAAGCTCCGGCGAGCTG
GTGATAAGCAAAGACCCGGAGGTATCCGAATGGGGAAACCCGGTAGAGTAATAGACTACCATTGCATGCTGAATACATAGGTATGCAAAG
CGACACCTGCCGAACTGAAACATCTTAGTAAGCAGAGGAAAAGAAATCGAAGAGATTCCCTGTGTAGCGGCGAGCGAAAGGGGAATAGCC
TAAACCGAGCTGATAAGGCTCGGGGTTGTAGGATTGAGGATAAAGGATCAGGACTCCTAGTTGAACACATCTGGAAAGATGGATGATACA
GGGTGATAGTCCCGTAGACGAAAGGAGAGAAAGACCGACCTCAACACCTGAGTAGGACTAGACACGTGAAACCTAGTCTGAATCTGGGGA
GACCACTCTCCAAGGCTAAATACTAGTCAATGACCGATAGTGAACCAGTACTGTGAAGGAAAGGCGAAAAGAACCCTTGTTAAGGGAGTG
AAATAGAACCTGAAACCAGTAGCTTACAAGCGGTCGGAGACCAATGGCCCGTAAGGGTCAAGGTTGACGGCGTGCCTTTTGCATGATGAG
CCAGGGAGTTAAGCTAAACGGCGAGGTTAAGGGATATACATTCCGGAGCCGGAGCGAAAGCGAGTTTTAAAAGAGCGAAGAGTCGTTTGG
TTTAGACACGAAACCAAGTGAGCTATTTATGACCAGGTTGAAGCATGGGTAAAACTATGTGGAGGACCGAACTAGTACCTGTTGAAAAAG
GTTTGGATGAGTTGTGAATAGGGGTGAAAGGCCAATCAAACTTGGAGATATCTTGTTCTCTCCGAAATAACTTTAGGGTTAGCCTCGGAT
AATAAGCTTTTGGGGGTAGAGCACTGAATTCTAGCGGGGGCCTACCGGCTTACCAACGGAAATCAAACTCCGAATACCAGAAGCGAGTCC
GGGAGATAGACAGCGGGGGCTAAGCTTCGTTGTCGAGAGGGGAACAGCCCAGACCGCCGATTAAGGTCCCTAATTTTATGCTAAGTGGGT
AAGGAAGTGATGATTCGAAGACAGTTGGAATGTTGGCTTAGAGGCAGCAATCATTTAAAGAGTGCGTAACAGCTCACCAATCGAGAATCA
TTGCGCCGATAATAAACGGGACTAAGCATAAAACCGACATCGCGGGTGTGTCGATAAGACACGCGGTAGGAGAGCGTAGTATTCAGCAGA
GAAGGTGTACCGGAAGGAGCGCTGGAGCGGATACTAGTGAAGATCCATGGCATAAGTAACGATAAAGGGAGTGAAAATCTCCCTCGCCGT
AAGCCCAAGGTTTCCAGGGTCAAGCTCGTCTTCCCTGGGTTAGTCGGCCCCTAAGTTGAGGCGTAACTGCGTAGACGATGGAGCAGCAGG
TTAAATATTCCTGCACCACCTAAAACTATAGCGAAGGAATGACGGAGTAAGTTAAGCACGCGGACGATTGGAAGAGTCCGTAGAGCGATG
AGAACGGTTAGTAGGCAAATCCGCTAACATAAGATCAGGTCGCGATCAAGGGGAATCTTCGGGGGAACCGATGGTGTGGAGCGAGGCTTT
CAAGAAATAATTTCTAGCTGTTGATGGTGACCGTACCAAAACCGACACAGGTGGGCGAGATGAATATTCTAAGGCGCGCGAGATAACTTT
CGTTAAGGAACTCGGCAAATTATCCCCGTAACTTCGGAATAAGGGGAGCCTTTTAGGGTGACTATGGAACGATAGGAGCCCCGGGGGGCC
GCAGAGAAATGGCCCAGGCGACTGTTTAGCAAAAACACAGCACTATGCAAACCTCTAAGGGGAAGTATATGGTGTGACGCCTGCCCAATG
CCAAAAGGTTAAAGGGATATGTCAGCTGCAAAGCGAAGCATTGAACCTAAGCCCTGGTGAATGGCCGCCGTAACTATAACGGTGCTAAGG
TAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGTGTAACGATCTGGGCACTGTCTCAACGAAAGACTCGGTGAAATTGTA
GTAGCAGTGAAGATGCTGTTTACCCGCGAAAGGACGAAAAGACCCCGTGAACCTTTACTGTACTTTGGTATTGATTTTTGGTTTGTTATG
TGTAGGATAGCCAGGAGACTAAGAACACTCTTCTTCAGGAGAGTGGGAGTCAACGTTGAAATACTGGTCTTAACAAGCTGGGAATCTAAC
ATTATTCCATGAATCTGGAAGATGGACATTGCCAGACGGGCAGTTTTACTGGGGCGGTATCCTCCTAAAAAGTAACGGAGGAGCCCAAAG
CTTATTTCATCGTGGTTGGCAATCACGAGTAGAGCGTAAAGGTATAAGATAGGTTGACTGCAAGACCAACAAGTCGAGCAGAGACGAAAG
TCGGGCTTAGTGATCCGGCGGTGGAAAGTGGAATCGCCGTCGCTTAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATCGCCACCAA
GAGTTCATATCGACGTGGCGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGGGCTGGAGAAGGTCCCAAGGGTTTGGCTGTTCGCC
AATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTCTCTATCCTTCGTGGGCGCAGGATACTTGAGAGGAGCTGT
TCCTAGTACGAGAGGACCGGAATGGACGAACCAATGGTGTGTCGGTTGTTTTGCCAAGGGCATAGCCGGGTGGCTCACGTTCGGAAAGGA
TAAGCATTGAAAGCATCTAAATGCCAAGCCTCCCTCAAGATAAGGTATCCCAATGAGACTCCATGTAGACTACGTGGTTGATAGGTTGGA
GGTGTAAGCACAGTAATGTGTTCAGCTAACCAATACTAATAAGTCCAAAGACTTGGTCTT