Escherichia coli ampC beta-lactamase

Accession ARO:3004290
CARD Short NameEcol_ampC_BLA
DefinitionA class C ampC beta-lactamase (cephalosporinase) enzyme described in Escherichia coli shown clinically to confer resistance to penicillin-like and cephalosporin-class antibiotics.
AMR Gene FamilyampC-type beta-lactamase
Drug Classcephalosporin, penam
Resistance Mechanismantibiotic inactivation
Resistomes with Perfect MatchesEscherichia colig+p+wgs, Klebsiella oxytocap, Shigella boydiiwgs, Shigella flexnerig+wgs, Shigella sonneiwgs
Resistomes with Sequence VariantsEscherichia albertiig+wgs, Escherichia colig+p+wgs, Escherichia marmotaewgs, Klebsiella oxytocap, Shigella boydiig+wgs, Shigella dysenteriaeg+wgs, Shigella flexnerig+wgs, Shigella sonneig+wgs
Classification14 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ ampC-type beta-lactamase [AMR Gene Family]
+ confers_resistance_to_antibiotic penicillin [Antibiotic]
Publications

Jacoby GA. 2009. Clin Microbiol Rev 22(1): 161-182. AmpC beta-lactamases. (PMID 19136439)

Abraham EP, et al. . Rev. Infect. Dis. 10(4):677-8 An enzyme from bacteria able to destroy penicillin. 1940. (PMID 3055168)

Resistomes

Prevalence of Escherichia coli ampC beta-lactamase among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Escherichia albertii90%0%56.77%0%
Escherichia coli12.91%0.01%9.58%0%
Escherichia marmotae0%0%4.17%0%
Klebsiella oxytoca0%0.68%0%0%
Shigella boydii80%0%81.11%0%
Shigella dysenteriae7.14%0%40%0%
Shigella flexneri72%0%73.76%0%
Shigella sonnei97.56%0%83.71%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 700

Type of Antibiotic Resistance: Intrinsic or chromosomally-encoded


>gb|AAC77110.1|-|Escherichia coli ampC beta-lactamase [Escherichia coli str. K-12 substr. MG1655]
MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGMAVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLG
GDAIARGEIKLSDPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAPGTQRLYANSSIGLFGALAVK
PSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWGYREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQL
AQSRYWQTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAITPPTPAVRASWVHKTGATGGFGSYVAFIPEKELGIVMLANKNY
PNPARVDAAWQILNALQ


>gb|U00096.3|-|4377811-4378944|Escherichia coli ampC beta-lactamase [Escherichia coli str. K-12 substr. MG1655]
ATGTTCAAAACGACGCTCTGCGCCTTATTAATTACCGCCTCTTGCTCCACATTTGCTGCCCCTCAACAAATCAACGATATTGTGCATCGC
ACAATTACCCCGCTTATAGAGCAACAAAAGATCCCGGGTATGGCGGTGGCGGTAATTTATCAGGGTAAACCTTATTACTTTACCTGGGGC
TATGCGGACATCGCCAAAAAGCAGCCCGTCACACAGCAAACGTTGTTTGAGTTAGGTTCGGTCAGCAAAACATTTACTGGCGTGCTTGGT
GGCGACGCTATTGCTCGAGGGGAAATCAAGTTAAGCGATCCCACAACAAAATACTGGCCTGAACTTACCGCTAAACAGTGGAATGGGATC
ACACTATTACATCTCGCAACCTACACTGCTGGCGGCCTGCCATTGCAGGTGCCGGATGAGGTGAAATCCTCAAGCGACTTGCTGCGCTTC
TATCAAAACTGGCAGCCTGCATGGGCTCCAGGAACACAACGTCTGTATGCCAACTCCAGTATCGGTTTGTTCGGCGCACTGGCTGTGAAG
CCGTCTGGTTTGAGTTTTGAGCAGGCGATGCAAACTCGTGTCTTCCAGCCACTCAAACTCAACCATACGTGGATTAATGTACCGCCCGCA
GAAGAAAAGAATTACGCCTGGGGATATCGCGAAGGTAAGGCAGTGCATGTTTCGCCTGGGGCGTTAGATGCTGAAGCTTATGGTGTGAAG
TCGACCATTGAAGATATGGCCCGCTGGGTGCAAAGCAATTTAAAACCCCTTGATATCAATGAGAAAACGCTTCAACAAGGGATACAACTG
GCACAATCTCGCTACTGGCAAACCGGCGATATGTATCAGGGCCTGGGCTGGGAAATGCTGGACTGGCCGGTAAATCCTGACAGCATCATT
AACGGCAGTGACAATAAAATTGCACTGGCAGCACGCCCCGTAAAAGCGATTACGCCCCCAACTCCTGCAGTACGCGCATCATGGGTACAT
AAAACAGGGGCGACCGGCGGATTTGGTAGCTATGTCGCGTTTATTCCAGAAAAAGAGCTGGGTATCGTGATGCTGGCAAACAAAAACTAT
CCCAATCCAGCGAGAGTCGACGCCGCCTGGCAGATTCTTAACGCTCTACAGTAA