Mycoplasma genitalium 23S rRNA mutations confers resistance to fluoroquinolone and macrolide antibiotics

Accession ARO:3004616
CARD Short NameMgen_23S_MULT
DefinitionMycoplasma genitalium strain M6320 contains mutations that lead to resistance to fluoroquinolone and macrolide antibiotics.
AMR Gene Family23S rRNA with mutation conferring resistance to macrolide antibiotics
Drug Classmacrolide antibiotic, fluoroquinolone antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Sequence VariantsMycoplasma genitaliumg, Mycoplasma pneumoniaeg+wgs
Classification11 ontology terms | Show
Parent Term(s)3 ontology terms | Show
+ confers_resistance_to_antibiotic moxifloxacin [Antibiotic]
+ confers_resistance_to_antibiotic azithromycin [Antibiotic]
+ 23S rRNA with mutation conferring resistance to macrolide antibiotics [AMR Gene Family]
Publications

Tagg KA, et al. 2013. J. Clin. Microbiol. 51(7):2245-9 Fluoroquinolone and macrolide resistance-associated mutations in Mycoplasma genitalium. (PMID 23658265)

Twin J, et al. 2012. PLoS ONE 7(4):e35593 Transmission and selection of macrolide resistant Mycoplasma genitalium infections detected by rapid high resolution melt analysis. (PMID 22532861)

Jensen JS, et al. 2008. Clin. Infect. Dis. 47(12):1546-53 Azithromycin treatment failure in Mycoplasma genitalium-positive patients with nongonococcal urethritis is associated with induced macrolide resistance. (PMID 18990060)

Resistomes

Prevalence of Mycoplasma genitalium 23S rRNA mutations confers resistance to fluoroquinolone and macrolide antibiotics among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 377 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: rRNA gene variant model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Mycoplasma genitalium20%0%0%0%
Mycoplasma pneumoniae36.71%0%25.89%0%
Show Perfect Only


Detection Models

Model Type: rRNA gene variant model

Model Definition: Ribosomal RNA (rRNA) Gene Variant Models (RVM) are similar to Protein Variant Models (PVM), i.e. detect sequences based on their similarity to a curated reference sequence and secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles, except RVMs are designed to detect AMR acquired via mutation of genes encoding ribosomal RNAs (rRNA). RVMs include a rRNA reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTN bit-score above the curated BLASTN cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTN bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastN): 4500

Legend:

  • discovered in clinical, agricultural, or environmental isolates

  • discovered via laboratory selection experiments

  • ReSeqTB https://platform.reseqtb.org

Published Variants:

PMID: 22532861A2071T
PMID: 18990060A2071C A2071G A2072G


>gb|L43967.2|+|171728-174461|Mycoplasma genitalium 23S rRNA mutations confers resistance to fluoroquinolone and macrolide antibiotics [Mycoplasma genitalium G37]
ACAATAAGTTACTAAGGGCTTATGGTGGATGCCTTGGCACTAAAAGGCGATGAAGGACGTGTTAACCTGCGATAAGCTTCGGGGAGGTGG
TAAAAACCTGAGATCCGGAGGTGTCCGAATGGAGCAATCTGGTAGCTTGCAAAAGTTACCATTAATTAATGAATTCATAGTTAATTAAAG
CGATACGTGGTGAAGTGAAACATCTCAGTAACCACAGGAAAAGAAAACGAATGTGATTCCGTGTGTAGTGGCGAGCGAAAGCGGAACAGG
CCAAACCTATCTGAGGATAGGGGTTGTAGGGCTTGCATTATGGAAGTTAAAAGGATAGAAGAAGCTGTTGGAAAGCAGCGCCAAAGAGGG
TGATAGCCCCGTATTTGAAATCTTTTTAATACCTAGCAAGAAACCTGAGTAGCTCGAAAAACGTTATTTTGAGTGAATCTGCCCAGACCA
TTGGGTAAGCCTAAATACTAATTAGTGACCGATAGCGAAACAGTACCGTGAGGGAAAGGTGAAAAGAACCCAGAGATGGGAGTGAAATAG
ATTCTGAAACCATATGCCTACAACGTGTCAGAGCACATTAATGTGTGATGGCGTGCGTTTTGAAGTATGAGCCGGCGAGTTATGATAGCA
AGCGTTAGTTAACCAGGAGATGGGGAGCTGTAGCGAAAGCGAGTTTTAAGAGAGCGTTTGTTTGTTATCATAGACCCGAAACGGGTTGAG
CTAGTCATGAGCAGGTTGAAGGTTGAGTAACATTAACTGGAGGACCGAACCGACTCTCGTTGAAACGATAGCGGATGACTTGTGATTAGG
GGTGAAATTCCAATCGAAATCCGTGATAGCTGGTTCTCGTCGAAATAGCTTTAAGGCTAGCGTAAGATCACAAATAAGTGGAGGTAAAGC
TACTGAATGTATGATGGCGCCACCTAGGCGTACTGAATACAATTAAACTCTGAATGCCATTTATTTTATTCTTGCAGTCAGACAGTGGGG
GATAAGCTTCATTGTCAAGAGGGGAAGAGCCCAGATCATTAAATAAGGTCCCCAAAATATACTAAGTGGAAAAGGATGTGAAAGTGCTAA
AACAGCAAGGATGTTGGCTTAGAAGCAGCCATCGTTTAAAGAGTGCGTAACAGCTCACTTGTCGAGTGTTTTTGCGCCGAAGATGTAACG
GGGCTAAGTATATTACCGAATTTATGGATAAGATATTTTTATCTTGTGGTAGACGAGCGTTGTATTGGAGTTGAAGTCAAAGCGTGAGCA
TTGGTGGATCCAATACAAGTGAGAATGCCGGCGTGAGTAACGCTTGGGAGTGAGAATCTCCCAAACCGATTGACTAAGGTTTCCTGGACC
AGGGTCGTCCTTCCAGGGTTAGTCTGGACCTAAGCTGAGGCTGAAGAGCGTAGGCGATGGACAACAGGTTAATATTCCTGTACTTACAGT
TAGACTGATGGAGTGACAAAGAAGGTTATCCACCCCCATTATTGGATTTGGGGATAAACCATAAGGTGGTACAATAGGCAAATCCGTTGT
GCATAACACTGAGTGGTGATGTCGAGTGAACGAGTGATCAAGTAGCGAAGGTGGCAATTAATCATGCTTTCAAGAAAAGCTGCTAGGGCT
AATTTAACTGTAACCAGTACCGAGAACGAACACACGTAGTCAAGGAGAGGATCCTAAGGTTAGCGAGTGAACTATAGCCAAGGAACTCTG
CAAATTAACCCCGTAAGTTAGCGAGAAGGGGTGCTTATCTAAAAGTAAGCCGCAGTGAAGAACGAGGGGGGACTGTTTAACTAAAACACA
ACTCTATGCCAAACCGTAAGGTGATGTATATGGGGTGACACCTGCCCAGTGCTGGAAGGTTAAAGAAGGAGGTTAGCAATTTATTGCAAA
GCTTTTAACTGAAGCCCCAGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTAGTCGGGTAAATTCCGTCCCGCTT
GAATGGTGTAACCATCTCTTGACTGTCTCGGCTATAGACTCGGTGAAATCCAGGTACGGGTGAAGACACCCGTTAGGCGCAACGGGACGG
AAAGACCCCGTGAAGCTTTACTGTAGCTTAATATTGATCAAAACACCACCATGTAGAGAATAGGTAGGAGCAATTGATGCAAGTTCGCAA
GGATTTGTTGATGTGAAATGTGGAATACTACCCTTGGTTATGTTTTGTTCTAATTGGTAACTGTGATCCAGTTTCAAGACAGTGTTAGGT
GGGCAGTTTGACTGGGGCGGTCGCCTCCTAAAAGGTAACGGAGGCGCACAAAGGTACCTTCAGTACGGTTGGAAATCGTATTTAGAGTGT
AATGGTATAAGGGTGCTTGACTGTGAGACTTACAGGTCGAACAGGTGAGAAATCAGGTCATAGTGATCCGGTGGTTCAGTATGGAATGGC
CATCGCTCAACGGATAAAAGCTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCAGTGTTTGGCACCTCGATGT
CGACTCATCTCATCCTCGAGCTGAAGCAGGTTCGAAGGGTTCGGCTGTTCGCCGATTAAAGAGATACGTGAGTTGGGTTCAAACCGTCGT
GAGACAGGTTGGTCCCTATCTATTGTGCCCACAGGAAGATTGAAGAGCTTTGCTTCTAGTACGAGAGGACCGGAGCGAGGACACCGCTTA
TGCTCCAGTTGTAGCGCCAGCTGCACCGCTGGGT