| Accession | ARO:3004659 |
| CARD Short Name | Mef(En2) |
| Definition | NBU2-encoded resistance gene. An MefE homolog in Bacteroides species. Macrolide efflux MFS transporter. |
| AMR Gene Family | major facilitator superfamily (MFS) antibiotic efflux pump |
| Drug Class | lincosamide antibiotic, macrolide antibiotic |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Resistomes with Sequence Variants | Bacteroides caccaewgs, Bacteroides fragilisg+wgs, Bacteroides ovatuswgs, Bacteroides thetaiotaomicrong+wgs, Parabacteroides distasoniswgs, Phocaeicola doreig+wgs, Phocaeicola massiliensiswgs, Phocaeicola vulgatusg+wgs, Riemerella anatipestiferg+wgs |
| Classification | 9 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic efflux [Resistance Mechanism] + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + antibiotic molecule + major facilitator superfamily (MFS) antibiotic efflux pump [AMR Gene Family] + lincosamide antibiotic [Drug Class] + macrolide antibiotic [Drug Class] |
| Parent Term(s) | 2 ontology terms | Show |
| Publications | Wang J, et al. 2000. J. Bacteriol. 182(12):3559-71 Characterization of a Bacteroides mobilizable transposon, NBU2, which carries a functional lincomycin resistance gene. (PMID 10852890) |
Prevalence of Mef(En2) among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Bacteroides caccae | 0% | 0% | 21.25% | 0% |
| Bacteroides fragilis | 26.92% | 0% | 15.12% | 0% |
| Bacteroides ovatus | 0% | 0% | 7.6% | 0% |
| Bacteroides thetaiotaomicron | 21.43% | 0% | 11.54% | 0% |
| Parabacteroides distasonis | 0% | 0% | 6.07% | 0% |
| Phocaeicola dorei | 18.18% | 0% | 45.83% | 0% |
| Phocaeicola massiliensis | 0% | 0% | 11.54% | 0% |
| Phocaeicola vulgatus | 16.67% | 0% | 21.45% | 0% |
| Riemerella anatipestifer | 5.56% | 0% | 2.08% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 700