Burkholderia dolosa gyrA conferring resistance to fluoroquinolones

Accession ARO:3004860
CARD Short NameBdol_gyrA_FLO
DefinitionPoint mutations in Burkholderia gyrA that confer resistance to ciprofloxacin, a fluoroquinolone antibiotic.
AMR Gene Familyfluoroquinolone resistant gyrA
Drug Classfluoroquinolone antibiotic
Resistance Mechanismantibiotic target alteration
Classification11 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic ciprofloxacin [Antibiotic]
+ fluoroquinolone resistant gyrA [AMR Gene Family]
Publications

Johnson SL, et al. 2015. Genome Announc 3(2): Complete genome sequences for 59 burkholderia isolates, both pathogenic and near neighbor. (PMID 25931592)

Tseng SP, et al. 2014. PLoS ONE 9(8):e104986 The contribution of antibiotic resistance mechanisms in clinical Burkholderia cepacia complex isolates: an emphasis on efflux pump activity. (PMID 25153194)

Resistomes

Prevalence of Burkholderia dolosa gyrA conferring resistance to fluoroquinolones among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
No prevalence data


Detection Models

Model Type: protein variant model

Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastP): 1700

Legend:

  • discovered in clinical, agricultural, or environmental isolates

  • discovered via laboratory selection experiments

  • ReSeqTB https://platform.reseqtb.org

Published Variants:

PMID: 25153194G81D T83I D87H

>gb|AJY14066.1|-|Burkholderia dolosa gyrA conferring resistance to fluoroquinolones [Burkholderia dolosa AU0158]
MDQFAKETLPTSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMHELNNDWN
RAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQDFSLRYMLIDGQGNFGSIDGDNAAAM
RYTEIRMAKIGHELLADIDKETVDFEPNYDGNETQPSVLPSRIPNLLINGSSGIAVGMAT
NIPPHNLNEVVDACQHLLSNPDATVDELIEIIPAPDFPTAGIIYGVAGVRDGYRTGRGRV
VMRAATHFEEIDRGQRMAIIVDELPYQVNKRSLLERIAELVNEKKLEGISDIRDESDKSG
MRVVIELKRGEVPEVVLNNLYKATQLQDTFGMNMVALVDGQPKLLNLKEILQCFLSHRRE
VLTRRTIYELRKARERGHVLEGLAVALANIDEFIAIIKAAPTPPIAKQELMTKSWDSSLV
REMLTRAESENAAAGGRAAYRPEGLNPAYGMQADGLYRLSDTQAQEILQMRLQRLTGLEQ
DKIIGEYREVMAQIADLLDILARPERITTMIGDELTTVKAEFGDARRSRIELNATELNTE
DLITPQDMVVTMSHAGYVKSQPLSEYRAQKRGGRGKQATQMKEDDWIETLFIANTHDYML
CFSNRGRVYWIKVYEVPQGSRNSRGRPIVNMFPLQEGEKINVVLPVKEFSADKFVFMATS
LGTVKKTPLEAFSRPMKKGIIAVGLDEGDYLIGASITDGAHDVMLFSDSGKAVRFDENDV
RPMGREARGVRGMQLEDGQQVIALLVAGSEEQSVLTATENGFGKRTPITEYTRHGRGTKG
MIAIQTSERNGKVVAATLVDPEDQIMLITTAGVLIRTRVSEIREMGRATQGVTLISLDEG
TKLSGLQQIAEAEEGDGEADEASDGEA



>gb|CP009795.1|-|2298743-2301346|Burkholderia dolosa gyrA conferring resistance to fluoroquinolones [Burkholderia dolosa AU0158]
ATGGATCAATTCGCCAAAGAGACCCTGCCCACCTCCCTCGAGGAGGAAATGCGCCGTTCGTATCTCGATTACGCGATGAGCGTGATCGTC
GGACGTGCCCTTCCGGATGTCCGCGATGGCCTGAAGCCCGTGCACCGGCGCGTACTGTTCGCGATGCACGAACTGAACAACGACTGGAAC
CGCGCGTACAAGAAATCGGCGCGTATCGTCGGCGACGTGATCGGTAAGTACCACCCGCACGGCGACACGGCGGTCTACGACACGATCGTG
CGGATGGCGCAGGACTTCTCGCTGCGCTACATGCTGATCGACGGGCAGGGCAACTTCGGCTCGATCGACGGCGACAATGCGGCGGCGATG
CGGTACACCGAAATTCGCATGGCGAAGATCGGGCACGAGCTGCTCGCCGACATCGACAAGGAAACGGTCGATTTCGAGCCGAACTACGAC
GGCAACGAAACGCAGCCGTCGGTCCTGCCGTCGCGGATCCCGAACCTGCTGATCAACGGCTCGTCGGGCATCGCCGTCGGCATGGCGACC
AACATTCCGCCGCACAACCTGAACGAAGTCGTCGACGCGTGCCAGCACCTGCTGAGCAACCCCGACGCGACGGTCGACGAACTGATCGAG
ATCATCCCGGCGCCGGATTTCCCGACGGCCGGCATCATCTACGGCGTCGCCGGCGTGCGCGACGGCTACCGCACCGGCCGCGGCCGCGTC
GTGATGCGCGCGGCCACGCACTTCGAGGAGATCGACCGCGGCCAGCGGATGGCGATCATCGTCGACGAGCTGCCGTACCAGGTCAACAAG
CGCTCGCTGCTCGAGCGGATCGCCGAGCTCGTCAACGAGAAGAAGCTCGAAGGCATTTCCGATATCCGCGACGAATCCGACAAGAGCGGC
ATGCGGGTCGTGATCGAGCTCAAGCGCGGCGAAGTGCCGGAAGTGGTGCTGAACAACCTGTACAAGGCGACGCAGCTGCAGGACACGTTC
GGCATGAACATGGTCGCGCTCGTCGACGGCCAGCCGAAGCTGCTGAACCTGAAGGAAATCCTGCAGTGCTTCCTGTCGCACCGGCGCGAA
GTGCTGACGCGCCGCACGATCTACGAACTGCGCAAGGCGCGCGAGCGCGGCCACGTGCTCGAAGGTCTCGCGGTCGCGCTCGCGAACATC
GACGAGTTCATCGCGATCATCAAGGCCGCGCCGACGCCGCCGATCGCGAAGCAGGAGCTGATGACGAAGTCGTGGGATTCGTCGCTCGTA
CGCGAGATGCTGACGCGCGCCGAATCCGAGAACGCGGCGGCGGGCGGCCGTGCCGCGTACCGTCCGGAAGGGCTGAATCCGGCGTACGGG
ATGCAGGCCGACGGGCTGTACAGGTTGTCCGACACGCAGGCGCAGGAAATCCTGCAGATGCGTCTGCAGCGCCTGACCGGCCTCGAGCAG
GACAAGATCATCGGCGAGTATCGCGAAGTGATGGCGCAGATCGCCGATCTGCTCGACATCCTCGCGCGCCCGGAGCGCATCACGACGATG
ATCGGCGACGAGCTGACGACGGTGAAGGCCGAATTCGGCGATGCGCGCCGCTCGCGGATCGAGCTGAACGCGACCGAACTGAATACCGAA
GACCTGATCACGCCGCAGGACATGGTCGTCACGATGTCGCATGCCGGCTACGTGAAGTCGCAGCCGCTGTCGGAGTACCGCGCGCAGAAG
CGCGGCGGCCGCGGCAAGCAGGCGACGCAGATGAAGGAAGACGACTGGATCGAGACGCTCTTCATCGCGAATACGCACGACTACATGCTG
TGCTTCTCGAACCGCGGTCGCGTGTACTGGATCAAGGTCTACGAGGTGCCGCAAGGTTCGCGCAACTCGCGCGGCCGTCCGATCGTCAAC
ATGTTCCCGCTGCAGGAAGGCGAGAAGATCAACGTCGTGCTGCCGGTGAAGGAATTCTCGGCCGACAAGTTCGTGTTCATGGCGACGTCG
CTCGGCACGGTCAAGAAGACGCCGCTCGAAGCGTTCAGCCGTCCGATGAAGAAGGGCATCATCGCGGTCGGCCTCGACGAGGGCGACTAT
CTGATCGGCGCGTCGATCACCGACGGCGCGCACGACGTGATGCTGTTCTCCGATTCGGGCAAGGCCGTGCGCTTCGACGAGAACGACGTG
CGTCCGATGGGCCGCGAAGCGCGCGGCGTGCGCGGCATGCAGCTCGAGGACGGGCAGCAGGTCATCGCGCTGCTGGTCGCGGGCAGCGAG
GAGCAGTCCGTGCTCACCGCAACCGAGAACGGCTTCGGCAAGCGTACGCCGATCACCGAGTACACGCGTCACGGCCGCGGCACGAAGGGT
ATGATCGCGATCCAGACCTCCGAGCGTAACGGCAAGGTCGTCGCTGCGACGCTCGTCGATCCGGAAGATCAGATCATGCTGATCACGACG
GCCGGCGTGTTGATTCGCACGCGCGTTTCCGAGATCCGCGAGATGGGACGGGCGACGCAAGGTGTTACACTCATCAGTCTCGATGAGGGT
ACGAAGCTTTCGGGTCTGCAGCAGATTGCCGAGGCCGAAGAGGGCGATGGCGAAGCCGACGAGGCGTCGGACGGCGAAGCCTGA