| Accession | ARO:3000230 |
| Synonym(s) | aadB |
| CARD Short Name | ANT(2'')-Ia |
| Definition | Plasmid or integron-encoded nucleotidylylation of 2-deoxystreptamine aminoglycosides at the hydroxyl group at position 2'' in P. aeruginosa, K. pneumoniae, Morganella morganii, E. coli, S. typhimurium, C. freundii and A. baumannii. |
| AMR Gene Family | ANT(2'') |
| Drug Class | aminoglycoside antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Achromobacter xylosoxidansp+wgs, Acinetobacter baumanniig+p+wgs+gi, Acinetobacter indicuswgs, Acinetobacter juniiwgs, Acinetobacter nosocomialiswgs, Acinetobacter pittiiwgs, Acinetobacter townerig, Aeromonas caviaeg+wgs, Aeromonas hydrophilap+wgs, Aeromonas veroniig+p, Alcaligenes faecalisg+wgs+gi, Burkholderia cenocepaciawgs, Burkholderia cepaciawgs, Burkholderia multivoranswgs, Burkholderia vietnamiensiswgs, Citrobacter freundiig+p+wgs, Citrobacter koseriwgs, Citrobacter portucalensiswgs, Citrobacter werkmaniiwgs, Enterobacter asburiaewgs, Enterobacter chengduensiswgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheig+p+wgs, Enterobacter kobeip+wgs, Enterobacter roggenkampiip+wgs, Escherichia albertiiwgs, Escherichia colig+p+wgs+gi, Klebsiella aerogeneswgs, Klebsiella michiganensisp+wgs, Klebsiella oxytocap+wgs, Klebsiella pneumoniaeg+p+wgs+gi, Klebsiella quasipneumoniaep+wgs, Morganella morganiig+wgs, Pasteurella multocidag+p+gi, Proteus mirabilisg+wgs+gi, Proteus vulgarisp, Providencia rettgerip+wgs, Providencia stuartiiwgs, Pseudomonas aeruginosag+p+wgs+gi, Pseudomonas monteiliiwgs, Pseudomonas putidap+wgs, Pseudomonas stutzerig+wgs, Salmonella entericag+p+wgs, Serratia marcescensp+wgs, Sphingobium yanoikuyaep, Stenotrophomonas maltophiliag+wgs, Trueperella pyogenesg+wgs+gi, Vibrio alginolyticusg+wgs, Vibrio parahaemolyticuswgs |
| Resistomes with Sequence Variants | Achromobacter xylosoxidansp+wgs, Acinetobacter baumanniig+p+wgs+gi, Acinetobacter indicuswgs, Acinetobacter johnsoniiwgs, Acinetobacter juniiwgs, Acinetobacter nosocomialiswgs, Acinetobacter pittiip+wgs, Acinetobacter townerig+wgs, Acinetobacter wuhouensisp, Aeromonas caviaeg+wgs, Aeromonas hydrophilap+wgs, Aeromonas veroniig+p, Alcaligenes faecalisg+wgs+gi, Burkholderia cenocepaciawgs, Burkholderia cepaciawgs, Burkholderia multivoranswgs, Burkholderia vietnamiensiswgs, Citrobacter freundiig+p+wgs+gi, Citrobacter koseriwgs, Citrobacter portucalensiswgs, Citrobacter werkmaniiwgs, Enterobacter asburiaewgs, Enterobacter chengduensiswgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheig+p+wgs, Enterobacter kobeip+wgs, Enterobacter roggenkampiip+wgs, Enterococcus faecaliswgs, Escherichia albertiiwgs, Escherichia colig+p+wgs+gi, Klebsiella aerogeneswgs, Klebsiella michiganensisp+wgs, Klebsiella oxytocap+wgs, Klebsiella pneumoniaeg+p+wgs+gi, Klebsiella quasipneumoniaeg+p+wgs, Morganella morganiig+wgs, Neisseria brasiliensisg+gi, Pasteurella multocidag+p+gi, Proteus mirabilisg+wgs+gi, Proteus vulgarisp, Providencia heimbachaep, Providencia rettgerip+wgs, Providencia stuartiiwgs, Pseudomonas aeruginosag+p+wgs+gi, Pseudomonas mendocinawgs, Pseudomonas monteiliiwgs, Pseudomonas putidap+wgs, Pseudomonas stutzerig+wgs, Salmonella entericag+p+wgs, Serratia marcescensp+wgs, Sphingobium yanoikuyaep, Stenotrophomonas maltophiliag+wgs, Trueperella pyogenesg+wgs+gi, Vibrio alginolyticusg+wgs, Vibrio parahaemolyticuswgs |
| Classification | 12 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + aminoglycoside modifying enzyme + nucleotidylation of antibiotic conferring resistance + antibiotic molecule + aminoglycoside nucleotidyltransferase (ANT) + aminoglycoside antibiotic [Drug Class] + ANT(2'') [AMR Gene Family] + antibiotic mixture |
| Parent Term(s) | 6 ontology terms | Show + confers_resistance_to_antibiotic dibekacin [Antibiotic] + confers_resistance_to_antibiotic sisomicin [Antibiotic] + confers_resistance_to_antibiotic kanamycin A [Antibiotic] + confers_resistance_to_antibiotic tobramycin [Antibiotic] + confers_resistance_to_antibiotic gentamicin [Antibiotic] + ANT(2'')-I |
| Publications | Cameron FH, et al. 1986. Nucleic Acids Res 14(21): 8625-8635. Nucleotide sequence of the AAD(2'') aminoglycoside adenylyltransferase determinant aadB. Evolutionary relationship of this region with those surrounding aadA in R538-1 and dhfrII in R388. (PMID 3024112) Cox G, et al. 2015. MBio 6(1): Structural and molecular basis for resistance to aminoglycoside antibiotics by the adenylyltransferase ANT(2″)-Ia. (PMID 25564464) |
Prevalence of ANT(2'')-Ia among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Achromobacter xylosoxidans | 0% | 14.29% | 0.76% | 0% |
| Acinetobacter baumannii | 5.49% | 1.92% | 7.73% | 3.14% |
| Acinetobacter indicus | 0% | 0% | 3.9% | 0% |
| Acinetobacter johnsonii | 0% | 0% | 1.82% | 0% |
| Acinetobacter junii | 0% | 0% | 2.99% | 0% |
| Acinetobacter nosocomialis | 0% | 0% | 1.72% | 0% |
| Acinetobacter pittii | 0% | 0.99% | 3.41% | 0% |
| Acinetobacter towneri | 12.5% | 0% | 3.85% | 0% |
| Acinetobacter wuhouensis | 0% | 4.55% | 0% | 0% |
| Aeromonas caviae | 4.55% | 0% | 0.54% | 0% |
| Aeromonas hydrophila | 0% | 1.3% | 1.61% | 0% |
| Aeromonas veronii | 1.82% | 1.54% | 0% | 0% |
| Alcaligenes faecalis | 5% | 0% | 17.65% | 33.33% |
| Burkholderia cenocepacia | 0% | 0% | 0.44% | 0% |
| Burkholderia cepacia | 0% | 0% | 6.73% | 0% |
| Burkholderia multivorans | 0% | 0% | 0.24% | 0% |
| Burkholderia vietnamiensis | 0% | 0% | 1.72% | 0% |
| Citrobacter freundii | 1.64% | 0.92% | 5.61% | 9.09% |
| Citrobacter koseri | 0% | 0% | 0.9% | 0% |
| Citrobacter portucalensis | 0% | 0% | 1.8% | 0% |
| Citrobacter werkmanii | 0% | 0% | 10.26% | 0% |
| Enterobacter asburiae | 0% | 0% | 5.14% | 0% |
| Enterobacter chengduensis | 0% | 0% | 8% | 0% |
| Enterobacter cloacae | 0% | 6.15% | 4.47% | 0% |
| Enterobacter hormaechei | 0.36% | 1.42% | 5.57% | 0% |
| Enterobacter kobei | 0% | 1.38% | 7.42% | 0% |
| Enterobacter roggenkampii | 0% | 0.97% | 5.04% | 0% |
| Enterococcus faecalis | 0% | 0% | 0.04% | 0% |
| Escherichia albertii | 0% | 0% | 0.65% | 0% |
| Escherichia coli | 0.14% | 0.06% | 0.65% | 0.26% |
| Klebsiella aerogenes | 0% | 0% | 0.85% | 0% |
| Klebsiella michiganensis | 0% | 1.14% | 1.33% | 0% |
| Klebsiella oxytoca | 0% | 4.11% | 1.68% | 0% |
| Klebsiella pneumoniae | 0.59% | 0.46% | 1.5% | 0.95% |
| Klebsiella quasipneumoniae | 0.84% | 1.48% | 2.89% | 0% |
| Morganella morganii | 9.62% | 0% | 4.29% | 0% |
| Neisseria brasiliensis | 100% | 0% | 0% | 50% |
| Pasteurella multocida | 4.29% | 2.17% | 0% | 16.67% |
| Proteus mirabilis | 1.83% | 0% | 3.96% | 3.7% |
| Proteus vulgaris | 0% | 22.22% | 0% | 0% |
| Providencia heimbachae | 0% | 100% | 0% | 0% |
| Providencia rettgeri | 0% | 5.41% | 10.19% | 0% |
| Providencia stuartii | 0% | 0% | 6.82% | 0% |
| Pseudomonas aeruginosa | 2.45% | 3.22% | 3.78% | 15.28% |
| Pseudomonas mendocina | 0% | 0% | 7.14% | 0% |
| Pseudomonas monteilii | 0% | 0% | 4.76% | 0% |
| Pseudomonas putida | 0% | 8% | 2.14% | 0% |
| Pseudomonas stutzeri | 3.57% | 0% | 5.34% | 0% |
| Salmonella enterica | 0.13% | 0.6% | 0.52% | 0% |
| Serratia marcescens | 0% | 0.65% | 5.11% | 0% |
| Sphingobium yanoikuyae | 0% | 4% | 0% | 0% |
| Stenotrophomonas maltophilia | 1.12% | 0% | 1.19% | 0% |
| Trueperella pyogenes | 6.67% | 0% | 8.33% | 10% |
| Vibrio alginolyticus | 1.23% | 0% | 1.19% | 0% |
| Vibrio parahaemolyticus | 0% | 0% | 0.36% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 300