KHM-1

Accession ARO:3000847
CARD Short NameKHM-1
DefinitionKHM-1 is a plasmid-mediated metallo-beta-lactamase found in Citrobacter freundii that confers resistance to all broad-spectrum beta-lactams, execpt for monobactams.
AMR Gene FamilyKHM beta-lactamase
Drug Classpenem, penam, cephamycin, cephalosporin, carbapenem
Resistance Mechanismantibiotic inactivation
Resistomes with Perfect MatchesEnterobacter hormaecheip, Klebsiella quasipneumoniaep
Resistomes with Sequence VariantsEnterobacter hormaecheip, Klebsiella quasipneumoniaep
Classification18 ontology terms | Show
Parent Term(s)1 ontology terms | Show
+ KHM beta-lactamase [AMR Gene Family]
Publications

Sekiguchi J, et al. 2008. Antimicrob Agents Chemother 52(11): 4194-4197. KHM-1, a novel plasmid-mediated metallo-beta-lactamase from a Citrobacter freundii clinical isolate. (PMID 18765691)

Resistomes

Prevalence of KHM-1 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Enterobacter hormaechei0%0.06%0%0%
Klebsiella quasipneumoniae0%0.21%0%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 400


>gb|BAF91108.1|+|KHM-1 [Citrobacter freundii]
MKIALVISFGLLLFTNMVCADDSLPELDIQKIEDGVYLYTAYEKIEGWGLVGSNGLVVLDNKNAYLIDTPISATDTEKLVKWIDAQGFTA
KASISTHFHTDSTGGIAFLNSKSIPTYASKLTNQLLKNKGEEQATHSFGKNPYWLLKNKIEAFYPGAGHTPDNLVVWLPKQKILFGGCFV
KPEGLGNLSHAVIAEWPASAEKLIARYSNATMVVPGHGKVGDASLLEKTRQRAVEALAAKK


>gb|AB364006.1|+|62-787|KHM-1 [Citrobacter freundii]
ATGAAAATAGCTCTTGTTATATCGTTTGGTCTGCTGTTGTTTACCAATATGGTATGCGCTGACGATTCATTACCAGAACTAGATATCCAA
AAAATAGAAGACGGCGTTTATCTGTACACCGCTTACGAAAAAATCGAAGGCTGGGGGCTTGTTGGCTCTAACGGATTAGTCGTGCTTGAT
AACAAAAATGCTTATCTGATTGATACGCCCATTTCAGCCACAGATACTGAAAAATTAGTGAAGTGGATTGACGCGCAGGGCTTTACGGCC
AAGGCAAGTATTTCTACCCATTTCCACACCGACAGTACAGGCGGTATTGCATTTCTCAACTCCAAGTCCATTCCAACCTATGCCTCCAAG
CTAACTAACCAGCTGCTTAAAAATAAAGGCGAAGAGCAGGCTACGCATTCGTTCGGTAAGAATCCTTATTGGCTATTAAAAAATAAAATC
GAAGCCTTTTATCCGGGTGCGGGTCACACACCTGATAATTTAGTAGTGTGGCTGCCGAAACAGAAAATTTTATTTGGTGGCTGTTTTGTC
AAACCCGAAGGCCTTGGCAATCTTAGCCATGCGGTAATTGCAGAATGGCCAGCTTCCGCCGAAAAACTTATCGCCCGTTATAGCAATGCA
ACAATGGTAGTTCCCGGTCACGGAAAAGTTGGCGACGCATCGCTGCTGGAAAAAACCAGGCAGCGCGCAGTTGAAGCGCTTGCAGCTAAA
AAGTGA