| Accession | ARO:3002982 |
| CARD Short Name | amrA |
| Definition | amrA is the efflux pump subunit of the AmrAB-OprM multidrug efflux complex. amrA corresponds to 1 locus in Pseudomonas aeruginosa PAO1 and 1 locus in Pseudomonas aeruginosa LESB58. |
| AMR Gene Family | resistance-nodulation-cell division (RND) antibiotic efflux pump |
| Drug Class | macrolide antibiotic, aminoglycoside antibiotic |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Resistomes with Perfect Matches | Burkholderia pseudomalleig+wgs |
| Resistomes with Sequence Variants | Burkholderia ambifariag+wgs, Burkholderia cenocepaciag+wgs+gi, Burkholderia cepaciag+wgs+gi, Burkholderia contaminansg+wgs+gi, Burkholderia dolosag+wgs+gi, Burkholderia latag+wgs, Burkholderia malleig+wgs, Burkholderia multivoransg+wgs+gi, Burkholderia oklahomensisg+wgs, Burkholderia pseudomalleig+wgs, Burkholderia thailandensisg+wgs, Burkholderia vietnamiensisg+p+wgs |
| Classification | 11 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic efflux [Resistance Mechanism] + determinant of antibiotic resistance + antibiotic molecule + macrolide antibiotic [Drug Class] + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + aminoglycoside antibiotic [Drug Class] + resistance-nodulation-cell division (RND) antibiotic efflux pump [AMR Gene Family] + azithromycin [Antibiotic] + tobramycin [Antibiotic] |
| Parent Term(s) | 2 ontology terms | Show |
| Publications | Jassem AN, et al. 2014. Ann Clin Microbiol Antimicrob 13(1): 2. Investigation of aminoglycoside resistance inducing conditions and a putative AmrAB-OprM efflux system in Burkholderia vietnamiensis. (PMID 24393536) Westbrock-Wadman S, et al. 1999. Antimicrob Agents Chemother 43(12): 2975-2983. Characterization of a Pseudomonas aeruginosa efflux pump contributing to aminoglycoside impermeability. (PMID 10582892) |
Prevalence of amrA among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Burkholderia ambifaria | 34.88% | 0% | 23.38% | 0% |
| Burkholderia cenocepacia | 34.67% | 0% | 68.86% | 50% |
| Burkholderia cepacia | 34.55% | 0% | 79.37% | 100% |
| Burkholderia contaminans | 32.65% | 0% | 53.75% | 40% |
| Burkholderia dolosa | 33.33% | 0% | 50% | 100% |
| Burkholderia lata | 33.33% | 0% | 37.5% | 0% |
| Burkholderia mallei | 40.68% | 0% | 42.86% | 0% |
| Burkholderia multivorans | 33.33% | 0% | 50.72% | 100% |
| Burkholderia oklahomensis | 50% | 0% | 83.33% | 0% |
| Burkholderia pseudomallei | 49.83% | 0% | 85.37% | 0% |
| Burkholderia thailandensis | 47.83% | 0% | 67.92% | 0% |
| Burkholderia vietnamiensis | 34.38% | 9.09% | 51.72% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 500