Neisseria gonorrhoeae gyrA with mutations conferring resistance to fluoroquinolones

Accession ARO:3003928
CARD Short NameNgon_gyrA_FLO
DefinitionPoint mutation in Neisseria gonorrhoeae DNA gyrase subunit A. Decreases affinity between fluoroquinolone antibiotic molecule and gyrA, thereby conferring resistance to fluoroquinolone.
AMR Gene Familyfluoroquinolone resistant gyrA
Drug Classfluoroquinolone antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Sequence VariantsNeisseria gonorrhoeaeg+p+wgs, Neisseria meningitidiswgs, Neisseria siccag+wgs
Classification11 ontology terms | Show
Parent Term(s)3 ontology terms | Show
+ confers_resistance_to_antibiotic ciprofloxacin [Antibiotic]
+ fluoroquinolone resistant gyrA [AMR Gene Family]
+ confers_resistance_to_antibiotic sitafloxacin [Antibiotic]
Publications

Kubanov A, et al. 2016. BMC Infect. Dis. 16:389 Molecular epidemiology of drug-resistant Neisseria gonorrhoeae in Russia (Current Status, 2015). (PMID 27506605)

Kivata MW, et al. 2019. BMC Microbiol. 19(1):76 gyrA and parC mutations in fluoroquinolone-resistant Neisseria gonorrhoeae isolates from Kenya. (PMID 30961546)

Ye M, et al. 2024. Antibiotics (Basel) 13(5): Emergence of Neisseria gonorrhoeae Clone with Reduced Susceptibility to Sitafloxacin in China: An In Vitro and Genomic Study. (PMID 38786196)

Resistomes

Prevalence of Neisseria gonorrhoeae gyrA with mutations conferring resistance to fluoroquinolones among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Neisseria gonorrhoeae50%1.02%41.56%0%
Neisseria meningitidis0%0%0.28%0%
Neisseria sicca33.33%0%7.69%0%
Show Perfect Only


Detection Models

Model Type: protein variant model

Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.

Bit-score Cut-off (blastP): 1500

Legend:

  • discovered in clinical, agricultural, or environmental isolates

  • discovered via laboratory selection experiments

  • ReSeqTB https://platform.reseqtb.org

Published Variants:

PMID: 27506605S91F D95G D95N
PMID: 38786196D95Y

>gb|AAW89357.1|-|Neisseria gonorrhoeae gyrA with mutations conferring resistance to fluoroquinolones [Neisseria gonorrhoeae FA 1090]
MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPDVRDGLKPVHRRVLYAM
HELKNNWNAAYKKSARIVGDVIGKYHPHGDSAVYDTIVRMAQNFAMRYVLIDGQGNFGSV
DGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGSEHEPLVLPTRFPTLLVNGSS
GIAVGMATNIPPHNLTDTINACLRLLDEPKTEIDELIDIIQAPDFPTGATIYGLGGVREG
YKTGRGRVVIRGKTHIEPIGKNGEREAIVIDEIPYQVNKAKLVEKIGDLVREKTLEGISE
LRDESDKSGMRVVIELKRNENAEVVLNQLYKLTPLQDSFGINMVVLVDGQPRLLNLKQIL
SEFLRHRREVVTRRTLFRLKKARHEGHIAEGKAVALSNIDEIIKLIKESPNAAEAKEKLL
ARPWRSSLVEEMLTRSGLDLEMMRPEGLAANIGLKKQGYYLSEIQADAILRMSLRNLTGL
DQKEIIESYKNLMGKIIDFVDILSKPERITQIIRDELEEIKTNYGDERRSEINPFGGDIA
DEDLIPQREMVVTLTHGGYIKTQPTTDYQAQRRGGRGKQAAATKDEDFIETLFVANTHDY
LMCFTNLGKCHWIKVYKLPEGGRNSRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFA
TAQGMVKKVQLSAFKNVRAQGIKAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEY
WEKSGNDEAEDADIETEISDDLEDETADNENTLPSGKNGVRPSGRGSGGLRGMRLPADGK
IVSLITFAPETEESGLQVLTATANGYGKRTPIADYSRKNKGGQGSIAINTGERNGDLVAA
TLVGETDDLMLITSGGVLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESEL
SGASVISNVTEPEAEN



>gb|AE004969.1|-|618439-621189|Neisseria gonorrhoeae gyrA with mutations conferring resistance to fluoroquinolones [Neisseria gonorrhoeae FA 1090]
ATGACCGACGCAACCATCCGCCACGACCACAAATTCGCCCTCGAAACCCTGCCCGTCAGCCTTGAAGACGAAATGCGCAAAAGCTATCTC
GACTACGCCATGAGCGTCATTGTCGGGCGCGCGCTGCCGGACGTTCGCGACGGCCTAAAGCCGGTGCACCGGCGCGTACTGTACGCGATG
CACGAGCTGAAAAATAACTGGAATGCCGCCTACAAAAAATCGGCGCGCATCGTCGGCGACGTCATCGGTAAATACCACCCCCACGGCGAT
TCCGCAGTTTACGACACCATCGTCCGTATGGCGCAAAATTTCGCTATGCGTTATGTGCTGATAGACGGACAGGGCAACTTCGGATCGGTG
GACGGGCTTGCCGCCGCAGCCATGCGCTATACCGAAATCCGCATGGCGAAAATCTCACATGAAATGCTGGCAGACATTGAGGAAGAAACC
GTTAATTTCGGCCCGAACTACGACGGTAGCGAACACGAGCCGCTTGTACTGCCGACCCGTTTCCCCACACTGCTCGTCAACGGCTCGTCC
GGTATCGCCGTCGGTATGGCGACCAACATCCCGCCGCACAACCTCACCGACACCATCAACGCCTGTCTGCGTCTTTTGGACGAACCCAAA
ACCGAAATCGACGAACTGATCGACATTATCCAAGCCCCCGACTTCCCGACCGGGGCAACCATCTACGGCTTGGGCGGCGTGCGCGAAGGC
TATAAAACAGGCCGCGGCCGCGTCGTTATACGCGGTAAGACCCATATCGAACCCATAGGCAAAAACGGCGAACGCGAAGCCATCGTTATC
GACGAAATCCCCTATCAGGTCAACAAAGCCAAGTTGGTCGAGAAAATCGGCGATTTGGTTCGGGAAAAAACGCTGGAAGGCATTTCCGAG
CTCCGCGACGAATCCGACAAATCCGGGATGCGCGTCGTTATCGAGCTGAAACGCAACGAAAATGCCGAAGTCGTCTTAAACCAACTCTAC
AAACTGACTCCGCTGCAAGACAGTTTCGGCATCAATATGGTTGTTTTGGTCGACGGACAACCGCGCCTGTTAAACCTGAAACAGATTCTC
TCCGAATTCCTGCGCCACCGCCGCGAAGTCGTTACCCGACGTACGCTTTTCCGGCTGAAGAAGGCACGCCATGAAGGGCATATCGCCGAA
GGCAAAGCCGTCGCACTGTCCAATATCGATGAAATCATCAAGCTCATCAAAGAATCGCCCAACGCGGCCGAGGCCAAAGAAAAACTGCTT
GCGCGCCCTTGGCGCAGCAGCCTCGTTGAAGAAATGCTGACGCGTTCCGGTCTGGATTTGGAAATGATGCGTCCGGAAGGATTGGCTGCA
AACATTGGTCTGAAAAAACAAGGTTATTACCTGAGCGAGATTCAGGCAGATGCTATTTTACGCATGAGCCTGCGAAACCTGACCGGCCTC
GATCAGAAAGAAATTATCGAAAGCTACAAAAACCTGATGGGTAAAATCATCGACTTTGTGGATATCCTCTCCAAACCCGAACGCATTACC
CAAATCATCCGTGACGAACTGGAAGAAATCAAAACCAACTATGGCGACGAACGCCGCAGCGAAATCAACCCGTTCGGCGGCGACATTGCC
GATGAAGACCTGATTCCGCAACGCGAAATGGTCGTGACCCTGACCCACGGCGGCTATATAAAAACCCAGCCGACCACCGACTATCAGGCT
CAGCGTCGCGGCGGGCGCGGCAAACAGGCGGCTGCCACCAAAGACGAAGACTTTATCGAAACCCTGTTTGTTGCCAACACGCATGACTAT
TTGATGTGTTTTACCAACCTCGGCAAGTGCCACTGGATTAAGGTTTACAAACTGCCCGAAGGCGGACGCAACAGCCGCGGCCGTCCGATT
AACAACGTCATCCAGCTGGAAGAAGGCGAAAAAGTCAGCGCGATTCTGGCAGTACGCGAGTTTCCCGAAGACCAATACGTCTTCTTCGCC
ACCGCGCAGGGAATGGTGAAAAAAGTCCAACTTTCCGCCTTTAAAAACGTCCGCGCCCAAGGCATTAAAGCCATCGCACTCAAAGAAGGC
GACTACCTCGTCGGCGCTGCGCAAACAGGCGGTGCGGACGACATTATGTTGTTCTCCAACTTGGGCAAAGCCATCCGCTTCAACGAATAC
TGGGAAAAATCCGGCAACGACGAAGCGGAAGATGCCGACATCGAAACCGAGATTTCAGACGACCTCGAAGACGAAACCGCCGACAACGAA
AACACCCTGCCAAGCGGCAAAAACGGCGTGCGTCCGTCCGGTCGCGGCAGCGGCGGTTTGCGCGGTATGCGCCTGCCTGCCGACGGCAAA
ATCGTCAGCCTGATTACCTTCGCCCCTGAAACCGAAGAAAGCGGTTTGCAAGTTTTAACCGCCACCGCCAACGGATACGGAAAACGCACC
CCGATTGCCGATTACAGCCGCAAAAACAAAGGCGGGCAAGGCAGTATTGCCATTAACACCGGCGAGCGCAACGGCGATTTGGTCGCCGCA
ACCTTGGTCGGCGAAACCGACGATTTGATGCTGATTACCAGCGGCGGCGTGCTTATCCGTACCAAAGTCGAACAAATCCGCGAAACCGGC
CGCGCCGCAGCAGGCGTGAAACTGATTAACTTGGACGAAGGCGAAACCTTGGTATCGCTGGAACGTGTTGCCGAAGACGAATCCGAACTC
TCCGGCGCTTCTGTAATTTCCAATGTAACCGAACCGGAAGCCGAGAACTGA