norB

Accession ARO:3000421
CARD Short NamenorB
DefinitionNorB is a multidrug efflux pump in Staphylococcus aureus that confers resistance to fluoroquinolones and other structurally unrelated antibiotics like tetracycline. It shares 30% similarity with NorB, and is a structural homolog of Blt of Bacillus subtilis. It is regulated by mgrA, also known as NorR.
AMR Gene Familymajor facilitator superfamily (MFS) antibiotic efflux pump
Drug Classfluoroquinolone antibiotic
Resistance Mechanismantibiotic efflux
Efflux Componentefflux pump complex or subunit conferring antibiotic resistance
Resistomes with Perfect MatchesListeria monocytogenesg+p+wgs
Resistomes with Sequence VariantsBacillus cereuswgs, Escherichia coliwgs, Klebsiella aerogeneswgs, Leptospira borgpeterseniiwgs, Listeria innocuag+wgs, Listeria monocytogenesg+p+wgs, Neisseria gonorrhoeaewgs
Classification7 ontology terms | Show
Parent Term(s)5 ontology terms | Show
+ confers_resistance_to_antibiotic ciprofloxacin [Antibiotic]
+ confers_resistance_to_antibiotic moxifloxacin [Antibiotic]
+ major facilitator superfamily (MFS) antibiotic efflux pump [AMR Gene Family]
+ confers_resistance_to_antibiotic norfloxacin [Antibiotic]
+ confers_resistance_to_antibiotic sparfloxacin [Antibiotic]
Sub-Term(s)
1 ontology terms | Show
+ mgrA regulates
Publications

Truong-Bolduc QC, et al. 2005. J Bacteriol 187(7): 2395-2405. MgrA is a multiple regulator of two new efflux pumps in Staphylococcus aureus. (PMID 15774883)

Resistomes

Prevalence of norB among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 377 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Bacillus cereus0%0%0.09%0%
Escherichia coli0%0%0.01%0%
Klebsiella aerogenes0%0%0.31%0%
Leptospira borgpetersenii0%0%1.03%0%
Listeria innocua100%0%50.54%0%
Listeria monocytogenes99.41%1.14%78.28%0%
Neisseria gonorrhoeae0%0%0.24%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 900


>gb|CCQ22388.1|-|norB [Listeria monocytogenes]
MTSTAYKGTNKLIVGIVFGVITFWLFAQSMVNIVPAVQSDLGISSDLLSIAISLTALFSGIFIVVAGGMADKFGRVKLTYIGLILSIIGS
LLLVVTQGSTLLIIGRIIQGLSAACIMPATLALMKTYFDGADRQRALSYWSIGSWGGSGICSFAGGAIATYMGWRWIFIISIVFALLGML
LIKGTPESKVVQNTKAKFDSFGLVLFVIAMVCLNLIITRGATFGWTSPITITMLVVFLVSAGLFFRVELRQANGFIDFSLFKNKAYTGAT
LSNFLLNAAAGTLVVANTYVQIGRGFTAFQSGLLSIGYLVCVLGMIRIGEKILQRVGARKPMILGSGITAVGIALMALTFIPGTLYTVLV
FIGFALFGIGLGMYATPSTDTAISNAPEDKVGVASGIYKMASSLGGSFGVAISATIYGVIALSGNIDLAAMVGLLTNVGFCVVSLISVAI
TTPSAKKALELKAAKE


>gb|HE999704.1|-|2734781-2736181|norB [Listeria monocytogenes]
ATGACTTCAACAGCGTATAAAGGTACAAATAAACTAATCGTTGGAATTGTTTTCGGGGTTATCACGTTTTGGCTTTTTGCTCAATCTATG
GTGAATATTGTTCCGGCCGTGCAATCTGACCTTGGAATTTCCTCTGATTTACTTAGTATTGCCATCAGTTTAACCGCGCTATTTTCAGGT
ATTTTTATCGTTGTAGCAGGTGGGATGGCTGACAAATTTGGTCGTGTGAAATTAACTTATATCGGACTTATTCTTAGTATCATCGGTTCA
CTGCTACTTGTTGTCACTCAAGGGTCGACGCTACTTATTATCGGCCGGATTATTCAAGGTCTTTCAGCTGCTTGTATTATGCCAGCAACC
CTTGCCTTAATGAAAACTTATTTTGACGGGGCAGATAGACAAAGAGCACTTAGTTACTGGTCAATTGGCTCATGGGGCGGATCAGGTATT
TGTTCGTTCGCAGGTGGCGCTATCGCAACATATATGGGCTGGCGCTGGATTTTCATTATTTCCATCGTATTCGCACTGCTTGGAATGCTA
CTTATTAAAGGTACTCCAGAAAGTAAAGTCGTTCAAAATACAAAAGCAAAATTTGATTCATTTGGTCTTGTTCTTTTTGTTATCGCAATG
GTTTGTTTGAACCTTATTATTACTCGTGGCGCAACATTTGGCTGGACAAGCCCAATTACTATTACAATGCTCGTTGTTTTCCTAGTTTCT
GCGGGATTATTCTTCCGAGTGGAACTGCGACAAGCAAACGGATTTATTGATTTCTCGTTATTTAAAAATAAAGCTTATACAGGCGCAACA
CTTTCGAACTTCTTGCTAAACGCAGCAGCTGGAACACTGGTTGTCGCAAACACTTATGTGCAAATTGGTCGCGGTTTTACGGCGTTCCAA
TCCGGTTTACTTTCTATCGGATATCTTGTCTGTGTGCTCGGAATGATTCGCATCGGTGAAAAAATTCTTCAACGTGTTGGTGCGCGTAAA
CCAATGATTTTAGGCTCTGGTATTACGGCTGTTGGTATTGCACTAATGGCGCTGACGTTTATTCCGGGAACCCTTTATACAGTGCTTGTA
TTTATCGGTTTTGCTTTATTCGGGATTGGACTTGGCATGTATGCGACTCCTTCAACAGATACAGCCATTTCTAATGCTCCAGAAGATAAA
GTCGGAGTAGCATCTGGTATTTACAAAATGGCAAGTTCGCTAGGTGGCTCATTCGGCGTGGCGATATCTGCTACGATTTATGGTGTGATT
GCACTTTCAGGAAATATTGATTTAGCCGCAATGGTGGGGCTTTTAACGAACGTCGGTTTTTGTGTCGTTTCACTTATTTCCGTTGCTATA
ACAACACCATCTGCGAAAAAAGCGCTCGAATTAAAAGCCGCAAAAGAATAG