| Accession | ARO:3000874 |
| CARD Short Name | TEM-2 |
| Definition | TEM-2 is a broad-spectrum beta-lactamase. |
| AMR Gene Family | TEM beta-lactamase |
| Drug Class | penam, monobactam, penem, cephalosporin |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Acinetobacter pittiip, Enterobacter hormaecheiwgs, Escherichia coliwgs, Klebsiella pneumoniaep+wgs, Proteus mirabilisg+wgs+gi, Providencia stuartiiwgs, Pseudomonas aeruginosawgs |
| Resistomes with Sequence Variants | Acinetobacter baumanniip, Acinetobacter pittiip, Enterobacter hormaecheiwgs, Escherichia coliwgs, Klebsiella pneumoniaep+wgs, Proteus mirabilisg+wgs+gi, Providencia stuartiiwgs, Pseudomonas aeruginosawgs |
| Classification | 17 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic molecule + determinant of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + hydrolysis of antibiotic conferring resistance + antibiotic inactivation enzyme + beta-lactam antibiotic + cephem + hydrolysis of beta-lactam antibiotic by serine beta-lactamase + penam [Drug Class] + beta-lactamase + class A beta-lactamase + monobactam [Drug Class] + ampicillin [Antibiotic] + penem [Drug Class] + cephalosporin [Drug Class] |
| Parent Term(s) | 2 ontology terms | Show |
| Sub-Term(s) | 2 ontology terms | Show + tazobactam [Adjuvant] is_small_molecule_inhibitor + clavulanic acid [Adjuvant] is_small_molecule_inhibitor |
| Publications | Ambler RP and Scott GK. 1978. Proc Natl Acad Sci U S A 75(8): 3732-3736. Partial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K. (PMID 358199) Matthew M and Hedges RW. 1976. J Bacteriol 125(2): 713-718. Analytical isoelectric focusing of R factor-determined beta-lactamases: correlation with plasmid compatibility. (PMID 1107329) |
Prevalence of TEM-2 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Acinetobacter baumannii | 0% | 0.05% | 0% | 0% |
| Acinetobacter pittii | 0% | 0.49% | 0% | 0% |
| Enterobacter hormaechei | 0% | 0% | 0.65% | 0% |
| Escherichia coli | 0% | 0% | 0.01% | 0% |
| Klebsiella pneumoniae | 0% | 0.02% | 0.02% | 0% |
| Proteus mirabilis | 0.92% | 0% | 0.5% | 3.7% |
| Providencia stuartii | 0% | 0% | 4.55% | 0% |
| Pseudomonas aeruginosa | 0% | 0% | 0.06% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 500