TEM-116

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Accession ARO:3000979
CARD Short NameTEM-116
DefinitionTEM-116 is a broad-spectrum beta-lactamase found in many species of bacteria.
AMR Gene FamilyTEM beta-lactamase
Drug Classpenicillin beta-lactam, cephalosporin, monobactam
Resistance Mechanismantibiotic inactivation
Resistomes with Perfect MatchesAcinetobacter baumanniiwgs, Aeromonas veroniiwgs, Bacillus subtilisg+wgs+gi, Bacillus thuringiensisp+wgs, Bacillus velezensiswgs, Bacteroides thetaiotaomicronwgs, Bifidobacterium bifidumwgs, Bifidobacterium longumwgs, Bordetella bronchisepticawgs, Brevibacillus laterosporuswgs, Burkholderia cepaciawgs, Burkholderia latawgs, Chlamydia trachomatisg+wgs, Clostridium botulinumwgs, Corynebacterium pseudotuberculosisg, Cronobacter dublinensiswgs, Cronobacter sakazakiiwgs, Enterococcus faecaliswgs, Enterococcus faeciumwgs, Escherichia albertiip, Escherichia colig+p+wgs, Escherichia marmotaewgs, Faecalibacterium prausnitziiwgs, Helicobacter pyloriwgs, Klebsiella michiganensiswgs, Klebsiella pneumoniaewgs, Klebsiella quasipneumoniaewgs, Legionella pneumophilawgs, Leptospira interroganswgs, Listeria monocytogeneswgs, Mycobacterium tuberculosiswgs, Mycobacterium ulceranswgs, Mycoplasma mycoidesg+gi, Propionibacterium freudenreichiig+gi, Pseudomonas aeruginosawgs, Pseudomonas putidag, Pseudomonas syringaewgs, Rhizobium leguminosarumwgs, Ruthenibacterium lactatiformanswgs, Salmonella entericawgs, Shigella flexneriwgs, Staphylococcus aureuswgs, Staphylococcus capitiswgs, Staphylococcus hominiswgs, Staphylococcus saprophyticuswgs, Streptococcus agalactiaewgs, Streptococcus pneumoniaewgs, Vibrio parahaemolyticuswgs, Vibrio vulnificuswgs, Yersinia kristenseniiwgs, Yersinia pseudotuberculosiswgs
Resistomes with Sequence VariantsAcinetobacter baumanniip+wgs, Aeromonas veroniiwgs, Bacillus subtilisg+wgs+gi, Bacillus thuringiensisp+wgs, Bacillus velezensiswgs, Bacteroides thetaiotaomicronwgs, Bifidobacterium bifidumwgs, Bifidobacterium dentiumwgs, Bifidobacterium longumwgs, Bordetella bronchisepticawgs, Brevibacillus laterosporuswgs, Burkholderia cepaciawgs, Burkholderia latawgs, Chlamydia trachomatisg+wgs, Clostridium botulinumwgs, Corynebacterium pseudotuberculosisg, Cronobacter dublinensiswgs, Cronobacter sakazakiiwgs, Enterococcus faecaliswgs, Enterococcus faeciumwgs, Escherichia albertiip, Escherichia colig+p+wgs, Escherichia marmotaewgs, Faecalibacterium prausnitziiwgs, Helicobacter pyloriwgs, Klebsiella michiganensiswgs, Klebsiella pneumoniaewgs, Klebsiella quasipneumoniaewgs, Legionella pneumophilawgs, Leptospira interroganswgs, Listeria monocytogeneswgs, Mycobacterium tuberculosiswgs, Mycobacterium ulceranswgs, Mycoplasma mycoidesg+gi, Propionibacterium freudenreichiig+gi, Pseudomonas aeruginosawgs, Pseudomonas putidag, Pseudomonas syringaewgs, Rhizobium leguminosarumwgs, Ruthenibacterium lactatiformanswgs, Salmonella entericawgs, Shigella flexneriwgs, Staphylococcus aureuswgs, Staphylococcus capitiswgs, Staphylococcus haemolyticuswgs, Staphylococcus hominiswgs, Staphylococcus saprophyticuswgs, Streptococcus agalactiaewgs, Streptococcus pneumoniaewgs, Vibrio choleraewgs, Vibrio parahaemolyticuswgs, Vibrio vulnificuswgs, Yersinia kristenseniiwgs, Yersinia pseudotuberculosiswgs
Classification17 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ confers_resistance_to_antibiotic ceftazidime [Antibiotic]
+ TEM beta-lactamase [AMR Gene Family]
Sub-Term(s)
1 ontology terms | Show
+ enmetazobactam [Adjuvant] is_small_molecule_inhibitor
Publications

Jeong SH, et al. 2004. J Clin Microbiol 42(7): 2902-2906. Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey. (PMID 15243036)
Resistomes

Prevalence of TEM-116 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GIGRDI-AMR2
Acinetobacter baumannii0%0.05%0.12%0%0%
Aeromonas veronii0%0%3.37%0%0%
Bacillus subtilis0.36%0%1.45%1.37%0%
Bacillus thuringiensis0%0.07%0.35%0%0%
Bacillus velezensis0%0%1.1%0%0%
Bacteroides thetaiotaomicron0%0%1.05%0%0%
Bifidobacterium bifidum0%0%1%0%0%
Bifidobacterium dentium0%0%2.27%0%0%
Bifidobacterium longum0%0%0.21%0%0%
Bordetella bronchiseptica0%0%1.37%0%0%
Brevibacillus laterosporus0%0%5.88%0%0%
Burkholderia cepacia0%0%0.9%0%0%
Burkholderia lata0%0%6.25%0%0%
Chlamydia trachomatis5.22%0%25.81%0%0%
Clostridium botulinum0%0%0.54%0%0%
Corynebacterium pseudotuberculosis4.43%0%0%0%0%
Cronobacter dublinensis0%0%2.56%0%0%
Cronobacter sakazakii0%0%1.57%0%0%
Enterococcus faecalis0%0%0.08%0%0%
Enterococcus faecium0%0%0.24%0%0%
Escherichia albertii0%1.69%0%0%0%
Escherichia coli0.05%0.01%0.08%0%0%
Escherichia marmotae0%0%2.08%0%0%
Faecalibacterium prausnitzii0%0%1.94%0%0%
Helicobacter pylori0%0%0.11%0%0%
Klebsiella michiganensis0%0%0.8%0%0%
Klebsiella pneumoniae0%0%0.06%0%0%
Klebsiella quasipneumoniae0%0%0.13%0%0%
Legionella pneumophila0%0%0.11%0%0%
Leptospira interrogans0%0%0.65%0%0%
Listeria monocytogenes0%0%0.02%0%0%
Mycobacterium tuberculosis0%0%0.08%0%0%
Mycobacterium ulcerans0%0%25%0%0%
Mycoplasma mycoides44%0%0%100%0%
Propionibacterium freudenreichii3.7%0%0%14.29%0%
Pseudomonas aeruginosa0%0%0.11%0%0%
Pseudomonas putida1.41%0%0%0%0%
Pseudomonas syringae0%0%1.02%0%0%
Rhizobium leguminosarum0%0%0.2%0%0%
Ruthenibacterium lactatiformans0%0%6.67%0%0%
Salmonella enterica0%0%0.11%0%0%
Shigella flexneri0%0%0.16%0%0%
Staphylococcus aureus0%0%0.07%0%0%
Staphylococcus capitis0%0%1.27%0%0%
Staphylococcus haemolyticus0%0%0.44%0%0%
Staphylococcus hominis0%0%0.49%0%0%
Staphylococcus saprophyticus0%0%0.7%0%0%
Streptococcus agalactiae0%0%0.13%0%0%
Streptococcus pneumoniae0%0%0.01%0%0%
Vibrio cholerae0%0%0.06%0%0%
Vibrio parahaemolyticus0%0%0.15%0%0%
Vibrio vulnificus0%0%0.82%0%0%
Yersinia kristensenii0%0%8.57%0%0%
Yersinia pseudotuberculosis0%0%5.88%0%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 500


>gb|AAB39956.1|+|TEM-116 [Staphylococcus aureus]
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG
RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTM
PVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDE
RNRQIAEIGASLIKHW


>gb|U36911.1|+|1430-2290|TEM-116 [Staphylococcus aureus]
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA

Curator Acknowledgements
Curator Description Most Recent Edit