TEM-116

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Accession	ARO:3000979
CARD Short Name	TEM-116
Definition	TEM-116 is a broad-spectrum beta-lactamase found in many species of bacteria.
AMR Gene Family	TEM beta-lactamase
Drug Class	penam, penem, cephalosporin, monobactam
Resistance Mechanism	antibiotic inactivation
Resistomes with Perfect Matches	Acinetobacter baumannii^wgs, Aeromonas veronii^wgs, Bacillus subtilis^g+wgs+gi, Bacillus thuringiensis^p+wgs, Bacillus velezensis^wgs, Bacteroides thetaiotaomicron^wgs, Bifidobacterium bifidum^wgs, Bifidobacterium longum^wgs, Bordetella bronchiseptica^wgs, Brevibacillus laterosporus^wgs, Burkholderia cepacia^wgs, Burkholderia lata^wgs, Chlamydia trachomatis^g+wgs, Clostridium botulinum^wgs, Corynebacterium pseudotuberculosis^g, Cronobacter dublinensis^wgs, Cronobacter sakazakii^wgs, Enterococcus faecalis^wgs, Enterococcus faecium^wgs, Escherichia albertii^p, Escherichia coli^g+p+wgs, Escherichia marmotae^wgs, Faecalibacterium prausnitzii^wgs, Helicobacter pylori^wgs, Klebsiella michiganensis^wgs, Klebsiella pneumoniae^wgs, Klebsiella quasipneumoniae^wgs, Legionella pneumophila^wgs, Leptospira interrogans^wgs, Listeria monocytogenes^wgs, Mycobacterium tuberculosis^wgs, Mycobacterium ulcerans^wgs, Mycoplasma mycoides^g+gi, Propionibacterium freudenreichii^g+gi, Pseudomonas aeruginosa^wgs, Pseudomonas putida^g, Pseudomonas syringae^wgs, Rhizobium leguminosarum^wgs, Ruthenibacterium lactatiformans^wgs, Salmonella enterica^wgs, Shigella flexneri^wgs, Staphylococcus aureus^wgs, Staphylococcus capitis^wgs, Staphylococcus hominis^wgs, Staphylococcus saprophyticus^wgs, Streptococcus agalactiae^wgs, Streptococcus pneumoniae^wgs, Vibrio parahaemolyticus^wgs, Vibrio vulnificus^wgs, Yersinia kristensenii^wgs, Yersinia pseudotuberculosis^wgs
Resistomes with Sequence Variants	Acinetobacter baumannii^p+wgs, Aeromonas veronii^wgs, Bacillus subtilis^g+wgs+gi, Bacillus thuringiensis^p+wgs, Bacillus velezensis^wgs, Bacteroides thetaiotaomicron^wgs, Bifidobacterium bifidum^wgs, Bifidobacterium dentium^wgs, Bifidobacterium longum^wgs, Bordetella bronchiseptica^wgs, Brevibacillus laterosporus^wgs, Burkholderia cepacia^wgs, Burkholderia lata^wgs, Chlamydia trachomatis^g+wgs, Clostridium botulinum^wgs, Corynebacterium pseudotuberculosis^g, Cronobacter dublinensis^wgs, Cronobacter sakazakii^wgs, Enterococcus faecalis^wgs, Enterococcus faecium^wgs, Escherichia albertii^p, Escherichia coli^g+p+wgs, Escherichia marmotae^wgs, Faecalibacterium prausnitzii^wgs, Helicobacter pylori^wgs, Klebsiella michiganensis^wgs, Klebsiella pneumoniae^wgs, Klebsiella quasipneumoniae^wgs, Legionella pneumophila^wgs, Leptospira interrogans^wgs, Listeria monocytogenes^wgs, Mycobacterium tuberculosis^wgs, Mycobacterium ulcerans^wgs, Mycoplasma mycoides^g+gi, Propionibacterium freudenreichii^g+gi, Pseudomonas aeruginosa^wgs, Pseudomonas putida^g, Pseudomonas syringae^wgs, Rhizobium leguminosarum^wgs, Ruthenibacterium lactatiformans^wgs, Salmonella enterica^wgs, Shigella flexneri^wgs, Staphylococcus aureus^wgs, Staphylococcus capitis^wgs, Staphylococcus haemolyticus^wgs, Staphylococcus hominis^wgs, Staphylococcus saprophyticus^wgs, Streptococcus agalactiae^wgs, Streptococcus pneumoniae^wgs, Vibrio cholerae^wgs, Vibrio parahaemolyticus^wgs, Vibrio vulnificus^wgs, Yersinia kristensenii^wgs, Yersinia pseudotuberculosis^wgs
Classification	17 ontology terms \| Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + hydrolysis of antibiotic conferring resistance + beta-lactam antibiotic + cephem + hydrolysis of beta-lactam antibiotic by serine beta-lactamase + penam [Drug Class] + beta-lactamase + penem [Drug Class] + ampicillin [Antibiotic] + class A beta-lactamase + cephalosporin [Drug Class] + monobactam [Drug Class]
Parent Term(s)	2 ontology terms \| Show + confers_resistance_to_antibiotic ceftazidime [Antibiotic] + TEM beta-lactamase [AMR Gene Family]
Sub-Term(s)	1 ontology terms \| Show + enmetazobactam [Adjuvant] is_small_molecule_inhibitor
Publications	Jeong SH, et al. 2004. J Clin Microbiol 42(7): 2902-2906. Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey. (PMID 15243036)

Resistomes

Prevalence of TEM-116 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

Species	NCBI Chromosome	NCBI Plasmid	NCBI WGS	NCBI GI
Acinetobacter baumannii	0%	0%	0.06%	0%
Acinetobacter baumannii	0%	0.05%	0.1%	0%
Aeromonas veronii	0%	0%	1.12%	0%
Aeromonas veronii	0%	0%	1.12%	0%
Bacillus subtilis	0.36%	0%	1.45%	1.37%
Bacillus subtilis	0.36%	0%	1.45%	1.37%
Bacillus thuringiensis	0%	0.07%	0.35%	0%
Bacillus thuringiensis	0%	0.07%	0.35%	0%
Bacillus velezensis	0%	0%	0.73%	0%
Bacillus velezensis	0%	0%	0.37%	0%
Bacteroides thetaiotaomicron	0%	0%	0.35%	0%
Bacteroides thetaiotaomicron	0%	0%	0.35%	0%
Bifidobacterium bifidum	0%	0%	1%	0%
Bifidobacterium bifidum	0%	0%	1%	0%
Bifidobacterium dentium	0%	0%	2.27%	0%
Bifidobacterium dentium	0%	0%	0%	0%
Bifidobacterium longum	0%	0%	0.21%	0%
Bifidobacterium longum	0%	0%	0.21%	0%
Bordetella bronchiseptica	0%	0%	1.37%	0%
Bordetella bronchiseptica	0%	0%	1.37%	0%
Brevibacillus laterosporus	0%	0%	5.88%	0%
Brevibacillus laterosporus	0%	0%	5.88%	0%
Burkholderia cepacia	0%	0%	0.9%	0%
Burkholderia cepacia	0%	0%	0.9%	0%
Burkholderia lata	0%	0%	6.25%	0%
Burkholderia lata	0%	0%	6.25%	0%
Chlamydia trachomatis	5.22%	0%	25.81%	0%
Chlamydia trachomatis	5.22%	0%	22.58%	0%
Clostridium botulinum	0%	0%	0.54%	0%
Clostridium botulinum	0%	0%	0.54%	0%
Corynebacterium pseudotuberculosis	4.43%	0%	0%	0%
Corynebacterium pseudotuberculosis	4.43%	0%	0%	0%
Cronobacter dublinensis	0%	0%	2.56%	0%
Cronobacter dublinensis	0%	0%	2.56%	0%
Cronobacter sakazakii	0%	0%	1.57%	0%
Cronobacter sakazakii	0%	0%	1.12%	0%
Enterococcus faecalis	0%	0%	0.04%	0%
Enterococcus faecalis	0%	0%	0.08%	0%
Enterococcus faecium	0%	0%	0.24%	0%
Enterococcus faecium	0%	0%	0.24%	0%
Escherichia albertii	0%	1.69%	0%	0%
Escherichia albertii	0%	1.69%	0%	0%
Escherichia coli	0.05%	0.01%	0.07%	0%
Escherichia coli	0.05%	0.01%	0.06%	0%
Escherichia marmotae	0%	0%	2.08%	0%
Escherichia marmotae	0%	0%	2.08%	0%
Faecalibacterium prausnitzii	0%	0%	1.94%	0%
Faecalibacterium prausnitzii	0%	0%	1.94%	0%
Helicobacter pylori	0%	0%	0.11%	0%
Helicobacter pylori	0%	0%	0.11%	0%
Klebsiella michiganensis	0%	0%	0.27%	0%
Klebsiella michiganensis	0%	0%	0.53%	0%
Klebsiella pneumoniae	0%	0%	0.03%	0%
Klebsiella pneumoniae	0%	0%	0.06%	0%
Klebsiella quasipneumoniae	0%	0%	0.13%	0%
Klebsiella quasipneumoniae	0%	0%	0.13%	0%
Legionella pneumophila	0%	0%	0.11%	0%
Legionella pneumophila	0%	0%	0.11%	0%
Leptospira interrogans	0%	0%	0.65%	0%
Leptospira interrogans	0%	0%	0.65%	0%
Listeria monocytogenes	0%	0%	0.02%	0%
Listeria monocytogenes	0%	0%	0.02%	0%
Mycobacterium tuberculosis	0%	0%	0.08%	0%
Mycobacterium tuberculosis	0%	0%	0.08%	0%
Mycobacterium ulcerans	0%	0%	25%	0%
Mycobacterium ulcerans	0%	0%	25%	0%
Mycoplasma mycoides	44%	0%	0%	100%
Mycoplasma mycoides	40%	0%	0%	89.47%
Propionibacterium freudenreichii	3.7%	0%	0%	14.29%
Propionibacterium freudenreichii	3.7%	0%	0%	14.29%
Pseudomonas aeruginosa	0%	0%	0.07%	0%
Pseudomonas aeruginosa	0%	0%	0.07%	0%
Pseudomonas putida	1.41%	0%	0%	0%
Pseudomonas putida	1.41%	0%	0%	0%
Pseudomonas syringae	0%	0%	0.68%	0%
Pseudomonas syringae	0%	0%	1.02%	0%
Rhizobium leguminosarum	0%	0%	0.2%	0%
Rhizobium leguminosarum	0%	0%	0.2%	0%
Ruthenibacterium lactatiformans	0%	0%	3.33%	0%
Ruthenibacterium lactatiformans	0%	0%	3.33%	0%
Salmonella enterica	0%	0%	0.1%	0%
Salmonella enterica	0%	0%	0.11%	0%
Shigella flexneri	0%	0%	0.16%	0%
Shigella flexneri	0%	0%	0.16%	0%
Staphylococcus aureus	0%	0%	0.06%	0%
Staphylococcus aureus	0%	0%	0.06%	0%
Staphylococcus capitis	0%	0%	1.27%	0%
Staphylococcus capitis	0%	0%	0.63%	0%
Staphylococcus haemolyticus	0%	0%	0%	0%
Staphylococcus haemolyticus	0%	0%	0.44%	0%
Staphylococcus hominis	0%	0%	0.49%	0%
Staphylococcus hominis	0%	0%	0.49%	0%
Staphylococcus saprophyticus	0%	0%	0.7%	0%
Staphylococcus saprophyticus	0%	0%	0.7%	0%
Streptococcus agalactiae	0%	0%	0.13%	0%
Streptococcus agalactiae	0%	0%	0.13%	0%
Streptococcus pneumoniae	0%	0%	0.01%	0%
Streptococcus pneumoniae	0%	0%	0.01%	0%
Vibrio cholerae	0%	0%	0.06%	0%
Vibrio cholerae	0%	0%	0%	0%
Vibrio parahaemolyticus	0%	0%	0.15%	0%
Vibrio parahaemolyticus	0%	0%	0.15%	0%
Vibrio vulnificus	0%	0%	0.41%	0%
Vibrio vulnificus	0%	0%	0.41%	0%
Yersinia kristensenii	0%	0%	5.71%	0%
Yersinia kristensenii	0%	0%	8.57%	0%
Yersinia pseudotuberculosis	0%	0%	4.41%	0%
Yersinia pseudotuberculosis	0%	0%	5.88%	0%

Show Perfect Only

Detection Models

protein homolog model

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 500

Protein
DNA

>gb|AAB39956.1|+|TEM-116 [Staphylococcus aureus]
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG
RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTM
PVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDE
RNRQIAEIGASLIKHW

>gb|U36911.1|+|1430-2290|TEM-116 [Staphylococcus aureus]
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA

TEM-116

Prevalence: protein homolog model (view sequences)

Graph