TEM-135

Accession ARO:3000999
CARD Short NameTEM-135
DefinitionTEM-135 is a broad-spectrum beta-lactamase found in Salmonella enterica.
AMR Gene FamilyTEM beta-lactamase
Drug Classpenam, monobactam, cephalosporin, penem
Resistance Mechanismantibiotic inactivation
Resistomes with Perfect MatchesCronobacter sakazakiip, Escherichia albertiiwgs, Escherichia colig+p+wgs, Escherichia fergusoniiwgs, Klebsiella pneumoniaewgs, Neisseria gonorrhoeaep+wgs, Salmonella entericap+wgs, Shigella sonneiwgs
Resistomes with Sequence VariantsCronobacter sakazakiip, Escherichia albertiiwgs, Escherichia colig+p+wgs, Escherichia fergusoniiwgs, Klebsiella pneumoniaewgs, Neisseria gonorrhoeaep+wgs, Salmonella entericap+wgs, Shigella sonneiwgs
Classification17 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ TEM beta-lactamase [AMR Gene Family]
+ confers_resistance_to_antibiotic penicillin [Antibiotic]
Publications

Pasquali F, et al. 2005. J Antimicrob Chemother 55(4): 562-565. Physical linkage of Tn3 and part of Tn1721 in a tetracycline and ampicillin resistance plasmid from Salmonella Typhimurium. (PMID 15731203)

Yan J, et al. 2019. Int. J. Antimicrob. Agents 54(3):361-366 High prevalence of TEM-135 expression from the Asian plasmid in penicillinase-producing Neisseria gonorrhoeae from Hangzhou, China. (PMID 31202926)

Resistomes

Prevalence of TEM-135 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Cronobacter sakazakii0%2.56%0%0%
Escherichia albertii0%0%0.65%0%
Escherichia coli0.02%0.23%0.25%0%
Escherichia fergusonii0%0%2.17%0%
Klebsiella pneumoniae0%0%0.09%0%
Neisseria gonorrhoeae0%5.1%1.44%0%
Salmonella enterica0%0.05%0.09%0%
Shigella sonnei0%0%0.29%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 500


>gb|CAG25427.1|-|TEM-135 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLG
RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTT
PAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDE
RNRQIAEIGASLIKHW


>gb|AJ634602.1|-|7840-8700|TEM-135 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGTATTCAACATTTTCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGTGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCTGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGACG
CCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA