| Accession | ARO:3002644 |
| Synonym(s) | aphA-2 |
| CARD Short Name | APH(3')-IIa |
| Definition | APH(3')-IIa is a transposon-encoded aminoglycoside phosphotransferase in E. coli. |
| AMR Gene Family | APH(3') |
| Drug Class | aminoglycoside antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Burkholderia glumaeg, Corynebacterium pseudotuberculosisg, Enterobacter asburiaeg, Escherichia albertiip, Escherichia colig+p, Escherichia fergusoniip, Klebsiella oxytocap, Klebsiella pneumoniaeg+p, Pseudomonas aeruginosag, Pseudomonas putidag, Salmonella entericag+p, Shigella flexnerip |
| Resistomes with Sequence Variants | Burkholderia glumaeg, Corynebacterium pseudotuberculosisg, Enterobacter asburiaeg, Escherichia albertiip, Escherichia colig+p, Escherichia fergusoniip, Klebsiella oxytocap, Klebsiella pneumoniaeg+p, Pseudomonas aeruginosag+p, Pseudomonas putidag, Rhodopseudomonas palustrisg, Salmonella entericag+p, Shigella flexnerip, Vibrio choleraeg, Xanthomonas oryzaeg |
| Classification | 13 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + determinant of antibiotic resistance + antibiotic inactivation enzyme + aminoglycoside modifying enzyme + antibiotic molecule + phosphorylation of antibiotic conferring resistance + aminoglycoside phosphotransferase (APH) + aminoglycoside antibiotic [Drug Class] + antibiotic mixture + gentamicin [Antibiotic] + APH(3') [AMR Gene Family] |
| Parent Term(s) | 7 ontology terms | Show + confers_resistance_to_antibiotic kanamycin A [Antibiotic] + confers_resistance_to_antibiotic neomycin [Antibiotic] + confers_resistance_to_antibiotic paromomycin [Antibiotic] + confers_resistance_to_antibiotic ribostamycin [Antibiotic] + confers_resistance_to_antibiotic butirosin [Antibiotic] + confers_resistance_to_antibiotic gentamicin B [Antibiotic] + APH(3')-II |
| Publications | Beck E, et al. 1982. Gene 19(3): 327-336. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. (PMID 6295884) |
Prevalence of APH(3')-IIa among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 381 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Burkholderia glumae | 8% | 0% | 0% | 0% |
| Corynebacterium pseudotuberculosis | 3.8% | 0% | 0% | 0% |
| Enterobacter asburiae | 3.23% | 0% | 0% | 0% |
| Escherichia albertii | 0% | 0.56% | 0% | 0% |
| Escherichia coli | 0.24% | 0.31% | 0% | 0% |
| Escherichia fergusonii | 0% | 2.14% | 0% | 0% |
| Klebsiella oxytoca | 0% | 0.68% | 0% | 0% |
| Klebsiella pneumoniae | 0.06% | 0.15% | 0% | 0% |
| Pseudomonas aeruginosa | 0.31% | 0.29% | 0% | 0% |
| Pseudomonas putida | 1.41% | 0% | 0% | 0% |
| Rhodopseudomonas palustris | 7.69% | 0% | 0% | 0% |
| Salmonella enterica | 0.44% | 1.59% | 0% | 0% |
| Shigella flexneri | 0% | 0.4% | 0% | 0% |
| Vibrio cholerae | 0.45% | 0% | 0% | 0% |
| Xanthomonas oryzae | 0.62% | 0% | 0% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 500
