Accession ARO:3002645
CARD Short NameAPH(3')-IIb
DefinitionAPH(3')-IIb is a chromosomal-encoded aminoglycoside phosphotransferase in P. aeruginosa.
AMR Gene FamilyAPH(3')
Drug Classaminoglycoside antibiotic
Resistance Mechanismantibiotic inactivation
Resistomes with Perfect MatchesPseudomonas aeruginosag
Resistomes with Sequence VariantsPseudomonas aeruginosag+p, Pseudomonas fluorescensg
Classification13 ontology terms | Show
Parent Term(s)7 ontology terms | Show
+ confers_resistance_to_antibiotic kanamycin A [Antibiotic]
+ confers_resistance_to_antibiotic neomycin [Antibiotic]
+ confers_resistance_to_antibiotic paromomycin [Antibiotic]
+ confers_resistance_to_antibiotic ribostamycin [Antibiotic]
+ confers_resistance_to_antibiotic butirosin [Antibiotic]
+ confers_resistance_to_antibiotic gentamicin B [Antibiotic]
+ APH(3')-II

Stover CK, et al. 2000. Nature 406(6799): 959-964. Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. (PMID 10984043)

Zeng L, et al. 2003. Antimicrob. Agents Chemother. 47(12):3867-76 aph(3')-IIb, a gene encoding an aminoglycoside-modifying enzyme, is under the positive control of surrogate regulator HpaA. (PMID 14638496)


Prevalence of APH(3')-IIb among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 381 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Pseudomonas aeruginosa98.16%0.29%0%0%
Pseudomonas fluorescens2.78%0%0%0%
Show Perfect Only

Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 500

>gb|CAA62365.1|+|APH(3')-IIb [Pseudomonas aeruginosa]

>gb|X90856.1|+|388-1194|APH(3')-IIb [Pseudomonas aeruginosa]