APH(3')-IVa

Accession ARO:3002648
Synonym(s)aphA-4
DefinitionAPH(3')-IVa is a chromosomal-encoded aminoglycoside phosphotransferase in B. circulans
AMR Gene FamilyAPH(3')
Drug Classaminoglycoside antibiotic
Resistance Mechanismantibiotic inactivation
Classification11 ontology terms | Show
Parent Term(s)6 ontology terms | Show
+ confers_resistance_to_antibiotic ribostamycin [Antibiotic]
+ confers_resistance_to_antibiotic butirosin [Antibiotic]
+ confers_resistance_to_antibiotic kanamycin A [Antibiotic]
+ APH(3') [AMR Gene Family]
+ confers_resistance_to_antibiotic gentamicin B [Antibiotic]
+ confers_resistance_to_antibiotic paromomycin [Antibiotic]
Publications

Herbert CJ, et al. 1986. Biochem J 233(2): 383-393. Sequence and interspecies transfer of an aminoglycoside phosphotransferase gene (APH) of Bacillus circulans. Self-defence mechanism in antibiotic-producing organisms. (PMID 3006668)

Resistomes

Prevalence of APH(3')-IVa among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 88 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
No prevalence data


Detection Models

Model Type: protein homolog model

Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.

Bit-score Cut-off (blastP): 500


>gb|CAA27061.1|+|APH(3')-IVa [Bacillus circulans]
MNESTRNWPEELLELLGQTELTVNKIGYSGDHVYHVKEYRGTPAFLKIAPSVWWRTLRPEIEALAWLDGKLPVPKILYTAEHGGMDYLLM
EALGGKDGSHETIQAKRKLFVKLYAEGLRSVHGLDIRECPLSNGLEKKLRDAKRIVDESLVDPADIKEEYDCTPEELYGLLLESKPVTED
LVFAHGDYCAPNLIIDGEKLSGFIDLGRAGVADRYQDISLAIRSLRHDYGDDRYKALFLELYGLDGLDEDKVRYYIRLDEFF


>gb|X03364|+|1-789|APH(3')-IVa [Bacillus circulans]
ATGAACGAAAGTACGCGTAATTGGCCGGAGGAACTTCTTGAGCTTCTCGGGCAGACGGAACTAACCGTCAACAAAATCGGATATTCCGGA
GATCACGTCTATCACGTGAAAGAGTACAGGGGCACCCCCGCATTTCTGAAAATCGCCCCCAGTGTATGGTGGAGAACGCTCCGGCCCGAA
ATTGAAGCGCTCGCTTGGCTGGACGGGAAGCTCCCGGTTCCCAAAATTTTGTACACGGCCGAACACGGCGGGATGGACTACTTGCTGATG
GAGGCGCTAGGCGGAAAAGACGGCTCCCACGAAACGATCCAGGCGAAGCGGAAACTGTTTGTGAAGCTGTACGCGGAAGGGCTCCGAAGC
GTGCATGGCCTCGATATCCGCGAATGTCCGCTGTCGAACGGGCTGGAGAAGAAGCTCCGGGATGCGAAAAGAATAGTCGATGAGAGCCTG
GTGGACCCGGCCGATATAAAAGAGGAGTACGATTGCACGCCGGAGGAATTGTACGGGCTATTGCTTGAGAGTAAGCCGGTAACCGAAGAT
CTGGTTTTTGCGCACGGAGATTACTGTGCTCCGAATCTGATTATCGACGGTGAGAAGCTGTCGGGATTTATCGATCTCGGACGTGCGGGC
GTGGCGGACCGTTATCAGGACATCAGCCTGGCGATCCGCAGCCTCCGGCACGATTACGGCGACGACCGCTACAAAGCGCTCTTCCTGGAA
CTTTACGGGCTGGACGGGCTTGACGAGGACAAGGTCCGGTATTACATCCGGCTGGATGAATTTTTTTGA