rmtH

Accession ARO:3003198
CARD Short NamermtH
DefinitionrmtH is a 16s ribosomal RNA methyltransferase found in Klebsiella pneumoniae strain MRSN2404 that was isolated from the chronic wound of a soldier wounded in Iraq in 2006. It confers high resistance to aminoglycosides.
AMR Gene Family16S rRNA methyltransferase (G1405)
Drug Classaminoglycoside antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Perfect MatchesKlebsiella pneumoniaewgs
Resistomes with Sequence VariantsKlebsiella pneumoniaewgs
Classification10 ontology terms | Show
Parent Term(s)5 ontology terms | Show
+ confers_resistance_to_antibiotic amikacin [Antibiotic]
+ confers_resistance_to_antibiotic tobramycin [Antibiotic]
+ confers_resistance_to_antibiotic arbekacin [Antibiotic]
+ confers_resistance_to_antibiotic gentamicin A [Antibiotic]
+ 16S rRNA methyltransferase (G1405) [AMR Gene Family]
Publications

O'Hara JA, et al. 2013. Antimicrob Agents Chemother 57(5): 2413-2416. Novel 16S rRNA methyltransferase RmtH produced by Klebsiella pneumoniae associated with war-related trauma. (PMID 23478957)

Resistomes

Prevalence of rmtH among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 377 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Klebsiella pneumoniae0%0%0.03%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 450


>gb|AGH19769.1|+|rmtH [Klebsiella pneumoniae]
MTIEQAAADILSSKKYQLLCPDTVVRILTQEWGRHKKPKQAVERTRERLHGICGAYLAPQVEKQASTALAAGDVQKALALHASTRERLDT
YPQLYQFVFENNLPARVLDIACGLNPLMLHRQGVASVWGCDIHQGLGNVLTPYAQKHGWDFTFALHDVLCAPVAASGDMALVFKLLPLLE
REQPGAALALLRTLDAPVICVSFPTRSLGGRGKGMHQHYATWFEGLVAPHFTVQHHTLIGDELLYRIQPNPA


>gb|KC544262.1|+|666-1424|rmtH [Klebsiella pneumoniae]
ATGACCATTGAACAGGCAGCGGCCGACATCCTCTCCTCAAAAAAATATCAACTGCTGTGCCCGGATACCGTGGTGCGCATCCTCACGCAG
GAGTGGGGACGCCACAAAAAGCCCAAGCAGGCGGTGGAGCGCACCCGCGAGCGGCTGCACGGCATCTGCGGTGCCTACCTGGCCCCCCAG
GTGGAAAAGCAGGCAAGCACCGCACTGGCTGCGGGCGATGTGCAAAAAGCGCTGGCACTGCATGCCTCCACCCGTGAGCGGCTGGATACC
TATCCCCAGCTGTATCAGTTTGTGTTTGAAAACAATCTGCCCGCCCGTGTGCTGGATATCGCCTGCGGCTTAAACCCGCTGATGCTGCAC
CGCCAGGGGGTGGCATCGGTTTGGGGGTGTGATATCCATCAGGGGCTGGGCAATGTGCTAACCCCCTATGCCCAAAAACACGGGTGGGAT
TTTACCTTTGCCCTGCACGATGTGCTGTGCGCACCGGTGGCGGCCAGCGGCGATATGGCACTGGTGTTTAAACTGCTGCCCCTTTTGGAA
AGAGAGCAGCCCGGCGCAGCCCTTGCGCTGCTGCGCACATTGGATGCCCCGGTGATCTGCGTCAGCTTCCCCACCCGCAGCTTGGGCGGC
AGGGGTAAGGGGATGCACCAGCACTACGCCACCTGGTTTGAGGGCCTGGTCGCCCCGCATTTTACCGTGCAGCACCACACCCTTATCGGG
GACGAGCTGCTTTACCGCATCCAGCCAAACCCAGCTTGA