| Accession | ARO:3003206 |
| CARD Short Name | lsaE |
| Definition | lsaE is an ABC-F subfamily protein found in porcine MRSA isolates. It confers resistance to pleuromutilin, lincosamide, and streptogramin A. |
| AMR Gene Family | lsa-type ABC-F protein |
| Drug Class | lincosamide antibiotic, pleuromutilin antibiotic, streptogramin antibiotic |
| Resistance Mechanism | antibiotic target protection |
| Resistomes with Perfect Matches | Campylobacter coliwgs, Campylobacter jejuniwgs, Enterococcus faecalisg+p+wgs, Enterococcus faeciump+wgs, Enterococcus hiraeg+p+wgs, Erysipelothrix rhusiopathiaeg+gi, Jeotgalibaca arthritidisg, Klebsiella pneumoniaewgs, Listeria innocuap, Staphylococcus aureusg+wgs+gi, Staphylococcus epidermidiswgs, Staphylococcus pseudintermediuswgs, Staphylococcus saprophyticusp+wgs, Streptococcus agalactiaeg+wgs, Streptococcus dysgalactiaeg+wgs, Streptococcus gallolyticuswgs, Streptococcus lutetiensiswgs, Streptococcus pasteurianuswgs, Streptococcus suisg+wgs, Streptococcus uberiswgs |
| Resistomes with Sequence Variants | Campylobacter coliwgs, Campylobacter jejuniwgs, Enterococcus faecalisg+p+wgs, Enterococcus faeciump+wgs, Enterococcus hiraeg+p+wgs, Erysipelothrix rhusiopathiaeg+gi, Jeotgalibaca arthritidisg, Klebsiella pneumoniaewgs, Listeria innocuap, Staphylococcus aureusg+wgs+gi, Staphylococcus epidermidiswgs, Staphylococcus pseudintermediuswgs, Staphylococcus saprophyticusp+wgs, Streptococcus agalactiaeg+wgs, Streptococcus dysgalactiaeg+wgs, Streptococcus equiwgs, Streptococcus gallolyticusg+wgs, Streptococcus lutetiensiswgs, Streptococcus pasteurianusg+wgs, Streptococcus porcinuswgs, Streptococcus suisg+wgs, Streptococcus uberiswgs |
| Classification | 10 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic target protection [Resistance Mechanism] + antibiotic molecule + antibiotic target protection protein + lincosamide antibiotic [Drug Class] + pleuromutilin antibiotic [Drug Class] + ABC-F ATP-binding cassette ribosomal protection protein + streptogramin antibiotic [Drug Class] |
| Parent Term(s) | 3 ontology terms | Show + confers_resistance_to_antibiotic pleuromutilin [Antibiotic] + lsa-type ABC-F protein [AMR Gene Family] + confers_resistance_to_antibiotic lincomycin [Antibiotic] |
| Publications | Li B, et al. 2013. J Antimicrob Chemother 68(6): 1251-1255. Detection and new genetic environment of the pleuromutilin-lincosamide-streptogramin A resistance gene lsa(E) in methicillin-resistant Staphylococcus aureus of swine origin. (PMID 23386262) Malbruny B, et al. 2011. Antimicrob Agents Chemother 55(4): 1470-1474. Cross-resistance to lincosamides, streptogramins A, and pleuromutilins due to the lsa(C) gene in Streptococcus agalactiae UCN70. (PMID 21245447) |
Prevalence of lsaE among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Campylobacter coli | 0% | 0% | 0.09% | 0% |
| Campylobacter jejuni | 0% | 0% | 0.05% | 0% |
| Enterococcus faecalis | 5.45% | 2.96% | 3.53% | 0% |
| Enterococcus faecium | 0% | 2.46% | 6.52% | 0% |
| Enterococcus hirae | 7.69% | 7.14% | 3.24% | 0% |
| Erysipelothrix rhusiopathiae | 27.27% | 0% | 0% | 66.67% |
| Jeotgalibaca arthritidis | 100% | 0% | 0% | 0% |
| Klebsiella pneumoniae | 0% | 0% | 0.01% | 0% |
| Listeria innocua | 0% | 10.53% | 0% | 0% |
| Staphylococcus aureus | 2.36% | 0% | 1.41% | 1.43% |
| Staphylococcus epidermidis | 0% | 0% | 0.08% | 0% |
| Staphylococcus pseudintermedius | 0% | 0% | 0.77% | 0% |
| Staphylococcus saprophyticus | 0% | 2.7% | 0.7% | 0% |
| Streptococcus agalactiae | 4.67% | 0% | 3.61% | 0% |
| Streptococcus dysgalactiae | 2% | 0% | 2.8% | 0% |
| Streptococcus equi | 0% | 0% | 0.23% | 0% |
| Streptococcus gallolyticus | 10% | 0% | 6.82% | 0% |
| Streptococcus lutetiensis | 0% | 0% | 2.04% | 0% |
| Streptococcus pasteurianus | 50% | 0% | 15% | 0% |
| Streptococcus porcinus | 0% | 0% | 33.33% | 0% |
| Streptococcus suis | 6.4% | 0% | 3.78% | 0% |
| Streptococcus uberis | 0% | 0% | 4.46% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 800