PAM-2

Accession ARO:3007200
CARD Short NamePAM-2
DefinitionPAM-2 is a member of the PAM family of subclass B3 metallo-beta-lactamases found in members of the Pseudomonas genus.
AMR Gene FamilyPAM beta-lactamase
Drug Classcarbapenem, cephalosporin
Resistance Mechanismantibiotic inactivation
Classification15 ontology terms | Show
Parent Term(s)2 ontology terms | Show
+ PAM beta-lactamase [AMR Gene Family]
+ confers_resistance_to_antibiotic ceftazidime [Antibiotic]
Publications

Yamada K, et al. 2021. J Glob Antimicrob Resist 24:395-397 Complete whole-genome sequence of the novel Pseudomonas species strain TUM18999, isolated from a patient with a burn wound in Japan. (PMID 33577996)

Resistomes

Prevalence of PAM-2 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
No prevalence data


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 450


>gb|GJN52392.1|+|PAM-2 [Pseudomonas tohonis]
MRLLASLALPLFAANLAVAAPAQLPQLEAYKGLDAWLVPVEPLRISDHVWQIGTASISALLVKTDAGAVLIDGGMPQVADHLLANMKKLG
VAPQDVRLILHSHAHIDHVGPLAAIKRATGAMLVSNAESAVLLQRGGANDIHFGSGMLFEPIQTDRLVQDGEAVTLGDTTFTVHFTPGHT
PGSMSWTWADTRDGKPLRIAYSDSLSAPGYQLRHNARYPHLVEAFRASFAAVRALPCDLLLTPHAEGSGWDYANAARPHPQPVSCKAYAD
KAEANLDKMLAEQANKR


>gb|BQKM01000003.1|+|545053-545916|PAM-2 [Pseudomonas tohonis]
ATGCGTTTGCTCGCCAGCCTCGCGCTGCCCCTGTTCGCCGCCAACCTGGCGGTCGCCGCCCCCGCCCAGCTGCCCCAGCTGGAGGCCTAC
AAGGGCCTCGACGCCTGGCTGGTGCCGGTGGAGCCCCTGCGCATCAGCGACCACGTGTGGCAGATCGGCACCGCCAGCATCAGCGCCCTG
CTGGTGAAGACCGACGCCGGCGCGGTGCTCATTGACGGCGGCATGCCCCAGGTGGCCGACCACCTGCTGGCCAACATGAAGAAGCTCGGC
GTGGCCCCGCAGGATGTGCGCCTGATCCTCCACAGCCACGCCCACATCGACCACGTCGGCCCGCTGGCGGCAATCAAGCGCGCCACCGGC
GCCATGCTGGTGAGCAACGCCGAATCCGCCGTGCTGCTGCAGCGCGGCGGCGCCAACGACATCCACTTCGGCAGCGGCATGCTCTTCGAA
CCGATCCAGACCGACCGCCTGGTGCAGGACGGCGAGGCCGTGACCCTGGGCGACACCACCTTCACCGTGCACTTCACCCCGGGGCACACG
CCCGGCAGCATGAGCTGGACCTGGGCCGACACCCGGGACGGCAAGCCCCTGCGCATCGCCTACAGCGACAGCCTCAGCGCGCCGGGCTAC
CAGCTGCGCCACAACGCCCGCTACCCGCACCTCGTGGAAGCCTTCCGCGCAAGCTTCGCCGCCGTCCGCGCCCTGCCCTGCGACCTGCTG
CTCACGCCCCACGCCGAAGGCAGCGGCTGGGACTACGCCAACGCCGCCAGGCCGCACCCGCAGCCGGTCAGCTGCAAGGCCTACGCGGAC
AAGGCCGAGGCGAATCTCGACAAGATGCTCGCCGAGCAGGCGAACAAGCGCTGA