| Accession | ARO:3007423 |
| CARD Short Name | Ecol_PBP3_BLA |
| Definition | Mutant PBP3 in E. coli conferring resistance to beta-lactams, including beta-lactam/beta-lactamase inhibitor combinations. |
| AMR Gene Family | Penicillin-binding protein mutations conferring resistance to beta-lactam antibiotics |
| Drug Class | penam, cephamycin, cephalosporin, monobactam |
| Resistance Mechanism | antibiotic target alteration |
| Classification | 14 ontology terms | Show + process or component of antibiotic biology or chemistry + antibiotic molecule + mechanism of antibiotic resistance + beta-lactam antibiotic + antibiotic target alteration [Resistance Mechanism] + cephem + mutation conferring antibiotic resistance + determinant of antibiotic resistance + beta-lactam resistant penicillin-binding proteins + penam [Drug Class] + cephamycin [Drug Class] + cephalosporin [Drug Class] + antibiotic resistant gene variant or mutant + monobactam [Drug Class] |
| Parent Term(s) | 4 ontology terms | Show + confers_resistance_to_antibiotic ceftazidime [Antibiotic] + confers_resistance_to_antibiotic aztreonam [Antibiotic] + confers_resistance_to_antibiotic ceftaroline [Antibiotic] + Penicillin-binding protein mutations conferring resistance to beta-lactam antibiotics [AMR Gene Family] |
| Publications | Zhang Y, et al. 2017. Antimicrob Agents Chemother 61(8): Unusual Escherichia coli PBP 3 Insertion Sequence Identified from a Collection of Carbapenem-Resistant Enterobacteriaceae Tested In Vitro with a Combination of Ceftazidime-, Ceftaroline-, or Aztreonam-Avibactam. (PMID 28559260) Alm RA, et al. 2015. J Antimicrob Chemother 70(5):1420-8 Characterization of Escherichia coli NDM isolates with decreased susceptibility to aztreonam/avibactam: role of a novel insertion in PBP3. (PMID 25634992) |
Prevalence of Escherichia coli PBP3 mutants conferring resistance to beta-lactam antibiotics among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| No prevalence data | ||||
Model Type: protein variant model
Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastP): 1100
Legend:
| PMID: 28559260 | +TIPY335-338 |
| PMID: 25634992 | +TIPY335-338 |