| Accession | ARO:3007659 |
| Synonym(s) | NiCoT NicT Rv2856 |
| CARD Short Name | Rv2856 |
| Definition | Ectopic expression of NiCoT in Escherichia coli CS109 resulted in the increase of intracellular nickel uptake with enhanced tolerance towards several antibiotics when NiCoT was overexpressed in E. coli and Mycobacterium smegmatis. The presence of a sub-inhibitory dose of nickel resulted in the manifestation of low-level tolerance towards these drugs. |
| AMR Gene Family | metal transporters with antibiotic efflux |
| Drug Class | isoniazid-like antibiotic, aminoglycoside antibiotic, fluoroquinolone antibiotic |
| Resistance Mechanism | antibiotic efflux |
| Efflux Component | efflux pump complex or subunit conferring antibiotic resistance |
| Classification | 10 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic efflux [Resistance Mechanism] + isoniazid-like antibiotic [Drug Class] + antibiotic mixture + efflux pump complex or subunit conferring antibiotic resistance [Efflux Component] + aminoglycoside antibiotic [Drug Class] + fluoroquinolone antibiotic [Drug Class] |
| Parent Term(s) | 7 ontology terms | Show + confers_resistance_to_antibiotic isoniazid [Antibiotic] + confers_resistance_to_antibiotic nalidixic acid [Antibiotic] + confers_resistance_to_antibiotic norfloxacin [Antibiotic] + confers_resistance_to_antibiotic ofloxacin [Antibiotic] + confers_resistance_to_antibiotic sparfloxacin [Antibiotic] + confers_resistance_to_antibiotic gentamicin [Antibiotic] + metal transporters with antibiotic efflux [AMR Gene Family] |
| Publications | Adhikary A, et al. 2022. Microbiology (Reading) 168(10): A NiCoT family metal transporter of Mycobacterium tuberculosis (Rv2856/NicT) behaves as a drug efflux pump that facilitates cross-resistance to antibiotics. (PMID 36282241) |
Prevalence of putative nickel/cobalt transporter among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| No prevalence data | ||||
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 700