| Accession | ARO:3001796 |
| CARD Short Name | OXA-50 |
| Definition | OXA-50 is a beta-lactamase found in Pseudomonas aeruginosa. It confers decreased susceptibility to ampicillin and ticarcillin and, interestingly, to moxalactam and meropenem in P. aeruginosa but not in E. coli. Also confers resistance to piperacillin-tazobactam and cephalotin. |
| AMR Gene Family | OXA beta-lactamase, OXA-50-like beta-lactamase |
| Drug Class | penam, cephalosporin |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Pseudomonas aeruginosag+wgs, Pseudomonas fluorescensg |
| Resistomes with Sequence Variants | Pseudomonas aeruginosag+wgs, Pseudomonas fluorescensg, Pseudomonas putidawgs |
| Classification | 16 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + hydrolysis of antibiotic conferring resistance + beta-lactam antibiotic + beta-lactamase + penam [Drug Class] + hydrolysis of beta-lactam antibiotic by serine beta-lactamase + oxacillin [Antibiotic] + cephem + class D beta-lactamase + OXA beta-lactamase [AMR Gene Family] + cephalosporin [Drug Class] |
| Parent Term(s) | 3 ontology terms | Show + confers_resistance_to_antibiotic ampicillin [Antibiotic] + confers_resistance_to_antibiotic cefalotin [Antibiotic] + OXA-50-like beta-lactamase [AMR Gene Family] |
| Publications | Girlich D, et al. 2004. Antimicrob Agents Chemother 48(6): 2043-2048. Biochemical characterization of the naturally occurring oxacillinase OXA-50 of Pseudomonas aeruginosa. (PMID 15155197) |
Prevalence of OXA-50 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Pseudomonas aeruginosa | 20.09% | 0% | 12.98% | 0% |
| Pseudomonas fluorescens | 2.78% | 0% | 0% | 0% |
| Pseudomonas putida | 0% | 0% | 0.53% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 300