| Accession | ARO:3001821 |
| CARD Short Name | ACT-1 |
| Definition | ACT-1 is a beta-lactamase found in Klebsiella pneumoniae. |
| AMR Gene Family | ACT beta-lactamase |
| Drug Class | penam, cephamycin, cephalosporin, carbapenem |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Enterobacter asburiaeg+wgs, Enterobacter cloacaewgs |
| Resistomes with Sequence Variants | Enterobacter asburiaeg+wgs, Enterobacter cloacaewgs |
| Classification | 16 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic molecule + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + hydrolysis of antibiotic conferring resistance + beta-lactam antibiotic + cephem + hydrolysis of beta-lactam antibiotic by serine beta-lactamase + beta-lactamase + class C beta-lactamase + penam [Drug Class] + cephamycin [Drug Class] + cephalosporin [Drug Class] + carbapenem [Drug Class] |
| Parent Term(s) | 1 ontology terms | Show + ACT beta-lactamase [AMR Gene Family] |
| Sub-Term(s) | 2 ontology terms | Show |
| Publications | Bradford PA, et al. 1997. Antimicrob Agents Chemother 41(3): 563-569. Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC beta-lactamase, and the foss of an outer membrane protein. (PMID 9055993) |
Prevalence of ACT-1 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Enterobacter asburiae | 6.45% | 0% | 9.88% | 0% |
| Enterobacter cloacae | 0% | 0% | 1.92% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 700