| Accession | ARO:3002597 |
| Synonym(s) | aac(6')-bifunctional aacA-aphD APH(2''), aph(2'')-bifunctional, AAC(6')-APH(2'') |
| Definition | AAC(6')-Ie-APH(2'')-Ia is an aminoglycoside acetyltransferase encoded by plasmids and transposons in S. aureus, E. faecalis, E. faecium and Staphylococcus warneri |
| AMR Gene Family | APH(2''), AAC(6') |
| Drug Class | aminoglycoside antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Campylobacter coliwgs, Campylobacter jejuniwgs, Clostridioides difficileg+wgs, Clostridium perfringensg+wgs, Enterobacter cloacaewgs, Enterococcus faecalisg+p+wgs, Enterococcus faeciumg+p+wgs, Escherichia coliwgs, Klebsiella aerogeneswgs, Klebsiella pneumoniaewgs, Staphylococcus aureusg+p+wgs, Staphylococcus epidermidisg+p+wgs, Staphylococcus pseudintermediusg+wgs, Streptococcus agalactiaeg+wgs, Streptococcus pneumoniaeg+wgs |
| Resistomes with Sequence Variants | Campylobacter colig+wgs, Campylobacter jejuniwgs, Clostridioides difficileg+wgs, Clostridium perfringensg+wgs, Enterobacter cloacaewgs, Enterococcus faecalisg+p+wgs, Enterococcus faeciumg+p+wgs, Escherichia coliwgs, Klebsiella aerogeneswgs, Klebsiella pneumoniaewgs, Morganella morganiip, Pseudomonas aeruginosawgs, Serratia marcescenswgs, Staphylococcus aureusg+p+wgs, Staphylococcus epidermidisg+p+wgs, Staphylococcus pseudintermediusg+wgs, Streptococcus agalactiaeg+wgs, Streptococcus pneumoniaeg+wgs |
| Classification | 23 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + antibiotic molecule + determinant of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + acylation of antibiotic conferring resistance + aminoglycoside antibiotic [Drug Class] + antibiotic mixture + phosphorylation of antibiotic conferring resistance + antibiotic inactivation enzyme + gentamicin B [Antibiotic] + tobramycin [Antibiotic] + isepamicin [Antibiotic] + amikacin [Antibiotic] + netilmicin [Antibiotic] + sisomicin [Antibiotic] + arbekacin [Antibiotic] + aminoglycoside acetyltransferase (AAC) + gentamicin C [Antibiotic] + aminoglycoside phosphotransferase (APH) + dibekacin [Antibiotic] + kanamycin A [Antibiotic] + neomycin [Antibiotic] |
| Parent Term(s) | 3 ontology terms | Show + APH(2'') [AMR Gene Family] + AAC(6') [AMR Gene Family] + confers_resistance_to_antibiotic plazomicin [Antibiotic] |
| Publications | Daigle DM, et al. 1999. Chem Biol 6(2): 99-110. Prodigious substrate specificity of AAC(6')-APH(2), an aminoglycoside antibiotic resistance determinant in enterococci and staphylococci. (PMID 10021417) Rouch DA, et al. 1987. J Gen Microbiol 133(11): 3039-3052. The aacA-aphD gentamicin and kanamycin resistance determinant of Tn4001 from Staphylococcus aureus: expression and nucleotide sequence analysis. (PMID 2833561) |
Prevalence of AAC(6')-Ie-APH(2'')-Ia among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 88 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS |
|---|---|---|---|
| Campylobacter coli | 5.56% | 0% | 1.97% |
| Campylobacter jejuni | 0% | 0% | 0.07% |
| Clostridioides difficile | 6.15% | 0% | 10.18% |
| Clostridium perfringens | 6.25% | 0% | 1.67% |
| Enterobacter cloacae | 0% | 0% | 0.78% |
| Enterococcus faecalis | 1.69% | 11.11% | 24.9% |
| Enterococcus faecium | 5.96% | 12.43% | 39.07% |
| Escherichia coli | 0% | 0% | 0.03% |
| Klebsiella aerogenes | 0% | 0% | 0.47% |
| Klebsiella pneumoniae | 0% | 0% | 0.08% |
| Morganella morganii | 0% | 5.26% | 0% |
| Pseudomonas aeruginosa | 0% | 0% | 0.02% |
| Serratia marcescens | 0% | 0% | 0.18% |
| Staphylococcus aureus | 11.38% | 3.4% | 17.3% |
| Staphylococcus epidermidis | 35.29% | 8.82% | 35.99% |
| Staphylococcus pseudintermedius | 40% | 0% | 65.58% |
| Streptococcus agalactiae | 1.09% | 0% | 2.75% |
| Streptococcus pneumoniae | 1.59% | 0% | 0.04% |
Model Type: protein homolog model
Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.
Bit-score Cut-off (blastP): 900