APH(2'')-IIIa

Accession ARO:3002636
Synonym(s)aph(2'')-Ic
CARD Short NameAPH(2'')-IIIa
DefinitionAPH(2'')-IIIa is a plasmid-encoded aminoglycoside phosphotransferase in Enterococcus gallinarum.
AMR Gene FamilyAPH(2'')
Drug Classaminoglycoside antibiotic
Resistance Mechanismantibiotic inactivation
Resistomes with Sequence VariantsCampylobacter coliwgs
Classification19 ontology terms | Show
Parent Term(s)5 ontology terms | Show
+ confers_resistance_to_antibiotic amikacin [Antibiotic]
+ confers_resistance_to_antibiotic kanamycin A [Antibiotic]
+ confers_resistance_to_antibiotic tobramycin [Antibiotic]
+ APH(2'') [AMR Gene Family]
+ confers_resistance_to_antibiotic gentamicin A [Antibiotic]
Publications

Chow JW, et al. 1997. Antimicrob Agents Chemother 41(3): 511-514. A novel gentamicin resistance gene in Enterococcus. (PMID 9055984)

Resistomes

Prevalence of APH(2'')-IIIa among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 377 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Campylobacter coli0%0%0.92%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 550


>gb|AAB49832.1|+|APH(2'')-IIIa [Enterococcus gallinarum]
MKQNKLHYTTMIMTQFPDISIQSVESLGEGFRNYAILVNGDWVFRFPKSQQGADELNKEIQLLPLLVGCVKVNIPQYVYIGKRSDGNPFV
GYRKVQGQILGEDGMAVFPDDAKDRLALQLAEFMNELSAFPVETAISAGVPVTNLKNKILLLSEAVEDQVFPLLDESLRDYLTLRFQSYM
THPVYTRYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYLLEDCGELFTRQVMAYRGEVDLDTHIRKVSLFVTFDQ
VSYLLEGLRARDQDWISEGLELLEEDKANNFGANSA


>gb|U51479.1|+|1-921|APH(2'')-IIIa [Enterococcus gallinarum]
ATGAAACAAAATAAACTTCACTATACCACAATGATAATGACTCAGTTCCCAGATATAAGCATACAATCCGTCGAGTCGCTTGGTGAGGGC
TTTAGGAATTACGCGATCCTCGTCAATGGAGATTGGGTTTTTCGTTTTCCCAAGAGTCAACAAGGTGCAGACGAATTGAACAAAGAAATC
CAATTGCTACCTCTGTTGGTCGGTTGTGTTAAGGTGAATATTCCACAGTATGTATATATCGGAAAGCGAAGTGATGGAAATCCCTTCGTG
GGCTACCGTAAAGTCCAAGGCCAAATCTTGGGTGAAGACGGGATGGCCGTTTTTCCCGATGATGCAAAAGATCGACTGGCGCTGCAACTT
GCTGAGTTCATGAATGAGCTAAGCGCATTTCCTGTTGAAACTGCCATATCAGCCGGGGTTCCTGTTACAAACCTGAAAAATAAAATTCTC
TTGCTATCGGAAGCTGTGGAGGATCAGGTGTTCCCTCTTCTTGATGAGTCTTTAAGGGACTATCTCACGCTGCGCTTCCAATCCTATATG
ACTCATCCGGTATATACACGATATACGCCGAGACTAATTCACGGCGATTTGTCACCTGATCATTTTTTGACGAATTTGAATTCACGTCAG
ACCCCATTAACAGGCATTATCGATTTTGGTGATGCCGCAATAAGTGATCCCGATTATGATTATGTATACCTTTTGGAAGATTGCGGCGAG
CTGTTTACTCGGCAAGTGATGGCTTATAGAGGCGAGGTTGACTTGGATACTCACATCAGAAAAGTCTCCTTGTTCGTAACGTTCGATCAA
GTCAGTTACCTGTTAGAAGGCTTAAGGGCAAGGGATCAGGACTGGATTTCTGAAGGGTTAGAGCTTTTGGAAGAGGATAAGGCCAACAAT
TTTGGTGCGAACAGTGCTTAA