APH(2'')-IVa

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Accession ARO:3002637
Synonym(s)aph(2'')-Id
CARD Short NameAPH(2'')-IVa
DefinitionAPH(2'')-IVa is a chromosomal-encoded aminoglycoside phosphotransferase in E. casseliflavus.
AMR Gene FamilyAPH(2'')
Drug Classaminoglycoside antibiotic
Resistance Mechanismantibiotic inactivation
Resistomes with Perfect MatchesEnterococcus faeciump+wgs
Resistomes with Sequence VariantsEnterococcus faeciump+wgs
Classification19 ontology terms | Show
Parent Term(s)5 ontology terms | Show
+ confers_resistance_to_antibiotic amikacin [Antibiotic]
+ confers_resistance_to_antibiotic kanamycin A [Antibiotic]
+ confers_resistance_to_antibiotic tobramycin [Antibiotic]
+ APH(2'') [AMR Gene Family]
+ confers_resistance_to_antibiotic gentamicin A [Antibiotic]
Publications

Tsai SF, et al. 1998. Antimicrob Agents Chemother 42(5): 1229-1232. A new high-level gentamicin resistance gene, aph(2'')-Id, in Enterococcus spp. (PMID 9593155)

Resistomes

Prevalence of APH(2'')-IVa among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 377 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Enterococcus faecium0%0.14%0.51%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 550


>gb|AAC14693.1|+|APH(2'')-IVa [Enterococcus casseliflavus]
MRTYTFDQVEKAIEQLYPDFTINTIEISGEGNDCIAYEINRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQM
SFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDI
LENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKY
WSFEKIIYGKEYGYMDWYEEGLNEIRSIKIK


>gb|AF016483.1|+|1-906|APH(2'')-IVa [Enterococcus casseliflavus]
ATGAGAACTTATACTTTCGACCAGGTAGAAAAGGCAATAGAGCAGTTATATCCTGATTTTACTATCAATACAATAGAGATTTCAGGAGAA
GGCAATGACTGTATTGCATATGAAATAAACAGGGATTTCATTTTTAAATTTCCAAAGCATTCAAGAGGATCTACTAATCTTTTTAATGAA
GTAAATATACTCAAAAGAATCCACAATAAATTACCCCTCCCCATTCCGGAGGTGGTTTTTACAGGAATGCCATCAGAAACGTACCAAATG
TCTTTCGCAGGTTTTACAAAAATTAAAGGAGTACCATTGACACCTCTTCTACTCAATAATCTGCCGAAGCAATCTCAAAATCAGGCAGCT
AAGGACCTGGCCCGATTTCTAAGTGAACTTCACAGCATAAACATCTCTGGATTCAAAAGTAATCTGGTATTAGATTTTCGAGAGAAGATA
AATGAAGATAATAAAAAAATCAAAAAGTTACTATCCAGGGAATTAAAGGGTCCCCAGATGAAGAAAGTGGATGATTTTTACAGGGATATT
CTAGAGAACGAAATCTACTTCAAATACTATCCTTGTCTTATTCATAACGATTTTAGCAGTGATCATATTTTATTTGATACCGAAAAAAAT
ACTATTTGTGGAATAATCGATTTTGGAGATGCAGCTATTTCTGATCCCGACAATGATTTTATAAGTTTGATGGAAGATGATGAAGAATAC
GGCATGGAATTTGTATCAAAAATATTGAACCATTACAAACATAAGGATATACCGACAGTTTTGGAAAAATATAGGATGAAAGAAAAATAC
TGGTCGTTCGAAAAGATTATCTATGGAAAGGAATATGGTTATATGGATTGGTATGAAGAGGGATTAAATGAAATCAGAAGCATTAAAATT
AAATAG