Mycobacterium leprae gyrB conferring resistance to fluoroquinolone

Accession ARO:3003304
DefinitionPoint mutation in Mycobacterium leprae gyrB resulting in fluoroquinolone resistance
AMR Gene Familyfluoroquinolone resistant gyrB
Drug Classfluoroquinolone antibiotic
Resistance Mechanismantibiotic target alteration
Classification12 ontology terms | Show
Parent Term(s)14 ontology terms | Show
+ fluoroquinolone resistant gyrB [AMR Gene Family]
+ confers_resistance_to_antibiotic ciprofloxacin [Antibiotic]
+ confers_resistance_to_antibiotic enoxacin [Antibiotic]
+ confers_resistance_to_antibiotic gatifloxacin [Antibiotic]
+ confers_resistance_to_antibiotic grepafloxacin [Antibiotic]
+ confers_resistance_to_antibiotic levofloxacin [Antibiotic]
+ confers_resistance_to_antibiotic lomefloxacin [Antibiotic]
+ confers_resistance_to_antibiotic moxifloxacin [Antibiotic]
+ confers_resistance_to_antibiotic nalidixic acid [Antibiotic]
+ confers_resistance_to_antibiotic norfloxacin [Antibiotic]
+ confers_resistance_to_antibiotic ofloxacin [Antibiotic]
+ confers_resistance_to_antibiotic pefloxacin [Antibiotic]
+ confers_resistance_to_antibiotic sparfloxacin [Antibiotic]
+ confers_resistance_to_antibiotic trovafloxacin [Antibiotic]
Publications

Yokoyama K, et al. 2012. PLoS Negl Trop Dis 6(10): E1838. Impact of amino acid substitutions in B subunit of DNA gyrase in Mycobacterium leprae on fluoroquinolone resistance. (PMID 23071850)

Resistomes

Prevalence of Mycobacterium leprae gyrB conferring resistance to fluoroquinolone among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 82 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
No prevalence data


Detection Models

Model Type: protein variant model

Model Definition: The protein variant model is an AMR detection model. Protein variant models are similar to protein homolog models - they detect the presence of a protein sequence based on its similarity to a curated reference sequence, but secondarily search submitted query sequences for curated sets of mutations shown clinically to confer resistance relative to wild-type. This model includes a protein reference sequence, a curated BLASTP cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of: single resistance variants, insertions, deletions, co-dependent resistance variants, nonsense SNPs, and/or frameshift mutations. Protein variant model matches to reference sequences are categorized on two criteria: strict and loose. A strict match has a BLASTP bitscore above the curated BLASTP cutoff value and contains at least one detected mutation from amongst the mapped resistance variants; a loose match has a BLASTP bitscore below the curated BLASTP cutoff value but still contains at least one detected mutation from amongst the mapped resistance variants. Regardless of BLASTP bitscore, if a sequence does not contain one of the mapped resistance variants, it is not considered a match and not detected by the protein variant model.

Legend:

  • discovered in clinical, agricultural, or environmental isolates
  • discovered via laboratory selection experiments


Bit-score Cut-off (blastP): 1200

PMID: 23071850D464N N502D E504V

>gb|CAC29513.1|+|Mycobacterium leprae gyrB conferring resistance to fluoroquinolone [Mycobacterium leprae TN]
MAAQRKAQDEYGAASITILEGLEAVRKRPGMYVGSTGERGLHHLIWEVVDNSVDEAMAGY
ATQVDVRLFDDGSVEVADNGRGIPVAVHATGVPTVDVVMTQLHAGGKFGGKDSGYNVSGG
LHGVGVSVVNALSTRVEVDIKRDGYEWSQFYDKAVPGILKQGEATEATGTTIRFWADPDI
FETTKYDFGTVARRIQEVAFLNKGLTINLVDERVKQDEVVDDVVSDTAEAPVAMTVEEKS
TESSAPHKVRHRTFHYPGGLVDFVKHINRTKTPIQQSIIDFDGKGAGHEVEVAMQWNGGY
SESVHTFANTINTHEGGTHEEGFRSALTSVVNKYAKDKKLLKDKDPNLTGDDIREGLAAV
ISVKVSEPQFEGQTKTKLGNTEVKSFVQRVCNEQLIHWFEANPVDAKAVVNKAISSAQAR
IAARKARELVRRKSATDLGGLPGKLADCRSTDPRSSELYVVEGDSAGGSAKSGRDSMFQA
ILPLRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDISRLRYHKIVLMADADVDG
QHISTLLLTLLFRFMRPLIEHGYVFLAQPPLYKLKWQRMDPEFAYSDSERDGLLETGLKL
GKKINKEDGIQRYKGLGEMDAKELWETTMDPSVRVLRQVTLDDAAAADELFSILMGEDVD
ARRSFITRNAKDVRFLDV



>gb|AL450380|+|5229-7265|Mycobacterium leprae gyrB conferring resistance to fluoroquinolone [Mycobacterium leprae TN]
GTGGCTGCCCAGAGGAAGGCCCAAGACGAATATGGCGCTGCGTCCATCACTATTCTTGAAGGGCTGGAGGCCGTTCGCAAACGTCCCGGT
ATGTACGTCGGGTCAACTGGTGAGCGTGGTCTCCACCATCTGATATGGGAAGTGGTGGACAACTCAGTAGATGAGGCGATGGCCGGTTAT
GCTACGCAAGTTGATGTGCGGTTATTCGACGACGGTAGTGTCGAGGTCGCCGATAACGGTCGTGGTATTCCGGTGGCAGTGCATGCTACG
GGGGTACCGACTGTTGACGTAGTTATGACCCAATTACATGCCGGCGGTAAATTCGGTGGTAAAGATAGCGGTTATAACGTCAGTGGTGGT
TTGCATGGGGTAGGTGTGTCGGTGGTTAACGCATTGTCCACCAGGGTCGAGGTCGACATCAAACGTGACGGCTATGAATGGTCACAGTTT
TACGACAAGGCTGTGCCGGGCATTCTTAAGCAAGGCGAAGCTACTGAGGCGACGGGAACGACGATTAGATTTTGGGCAGATCCTGACATT
TTCGAAACCACAAAGTATGACTTTGGGACGGTGGCGCGCCGAATTCAAGAAGTGGCTTTCTTGAACAAGGGTTTGACGATCAATCTTGTT
GACGAACGGGTGAAGCAGGACGAAGTTGTCGACGATGTCGTCAGCGATACAGCCGAGGCTCCTGTGGCTATGACCGTTGAAGAAAAGTCA
ACGGAGTCGAGTGCGCCGCACAAGGTTAGACACCGTACGTTCCACTACCCCGGAGGCCTGGTGGACTTCGTCAAGCACATCAACCGGACT
AAGACTCCTATTCAACAGAGCATTATCGATTTTGATGGCAAAGGTGCCGGTCACGAGGTTGAAGTTGCGATGCAGTGGAACGGCGGCTAT
TCGGAATCAGTGCATACCTTTGCGAACACGATTAACACCCATGAAGGCGGCACCCACGAAGAAGGTTTCCGTAGCGCTTTGACATCAGTG
GTGAACAAGTACGCTAAGGATAAAAAACTACTCAAAGACAAGGATCCCAACCTAACTGGCGACGATATCCGTGAAGGTCTGGCGGCGGTT
ATCTCGGTTAAGGTCAGTGAACCACAGTTTGAGGGTCAGACCAAAACAAAGCTGGGGAACACCGAAGTTAAGTCATTCGTGCAGAGGGTC
TGTAATGAGCAACTTATTCACTGGTTTGAAGCCAATCCAGTAGATGCGAAAGCGGTTGTGAATAAGGCGATATCGTCGGCACAAGCCCGA
ATAGCTGCACGTAAAGCACGAGAGTTAGTGCGTCGAAAAAGTGCCACCGATCTTGGTGGACTTCCTGGAAAACTTGCCGATTGCCGCTCT
ACTGATCCTCGAAGTTCTGAACTGTATGTAGTGGAAGGTGATTCGGCTGGTGGTTCAGCAAAGAGTGGCCGTGATTCGATGTTTCAGGCA
ATCCTTCCGTTACGTGGCAAGATCATAAATGTTGAAAAGGCACGTATTGACCGAGTGCTAAAGAACACCGAAGTTCAAGCAATTATTACG
GCATTGGGTACTGGAATCCATGATGAATTCGATATCTCCAGGCTGCGTTATCACAAAATTGTTTTGATGGCCGACGCTGACGTTGACGGC
CAACATATCTCGACGCTGTTGTTGACTTTGTTATTTCGGTTCATGCGACCACTCATCGAGCATGGGTACGTGTTTTTAGCGCAGCCGCCA
CTTTACAAATTGAAGTGGCAACGTATGGATCCGGAATTTGCTTACTCCGATAGCGAGCGCGACGGCTTATTAGAGACCGGGCTTAAGCTT
GGCAAGAAAATCAACAAAGAGGATGGTATCCAACGTTATAAAGGTTTAGGTGAAATGGATGCCAAGGAGTTGTGGGAAACCACCATGGAC
CCGTCGGTGCGAGTTTTGCGTCAAGTAACACTGGATGACGCGGCGGCTGCTGACGAGTTATTCTCTATTCTGATGGGTGAGGACGTCGAT
GCACGCCGTAGCTTTATCACCCGTAATGCCAAGGATGTTCGTTTCCTGGATGTCTAG