| Accession | ARO:3003390 |
| CARD Short Name | Ecol_ompF_BLA |
| Definition | The Escherichia coli ompF (oprF) is a nonspecific porin channel involved in the membrane translocation of small hydrophilic molecules, including and especially beta-lactam antibiotics. Mutations in ompF can decrease diffusion of antibiotics across the cellular membrane, thereby decreasing overall susceptibility through absence of porin function. |
| AMR Gene Family | General Bacterial Porin with reduced permeability to beta-lactams |
| Drug Class | penicillin beta-lactam, cephalosporin, carbapenem, monobactam |
| Resistance Mechanism | reduced permeability to antibiotic |
| Resistomes with Sequence Variants | Escherichia coliwgs |
| Classification | 14 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + reduced permeability to antibiotic [Resistance Mechanism] + determinant of antibiotic resistance + antibiotic molecule + protein modulating permeability to antibiotic + beta-lactam antibiotic + General Bacterial Porin (GBP) + penicillin beta-lactam [Drug Class] + cephalosporin [Drug Class] + carbapenem [Drug Class] + monobactam [Drug Class] + fourth-generation cephalosporin + General Bacterial Porin with reduced permeability to beta-lactams [AMR Gene Family] |
| Parent Term(s) | 2 ontology terms | Show |
| Publications | Simonet V, et al. 2000. Antimicrob Agents Chemother 44(2): 311-315. Substitutions in the eyelet region disrupt cefepime diffusion through the Escherichia coli OmpF channel. (PMID 10639355) |
Prevalence of Escherichia coli ompF with mutation conferring resistance to beta-lactam antibiotics among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI | GRDI-AMR2 |
|---|---|---|---|---|---|
| Escherichia coli | 0% | 0% | 0.01% | 0% | 0% |
Model Type: protein variant model
Model Definition: Protein Variant Models (PVM) perform a similar search as Protein Homolog Models (PHM), i.e. detect protein sequences based on their similarity to a curated reference sequence, but secondarily screen query sequences for curated sets of mutations to differentiate them from antibiotic susceptible wild-type alleles. PVMs are designed to detect AMR acquired via mutation of house-keeping genes or antibiotic targets, e.g. a mutated gyrase resistant to aminocoumarin antibiotics. PVMs include a protein reference sequence (often from antibiotic susceptible wild-type alleles), a curated bit-score cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of single point mutations, insertions, or deletions curated from the scientific literature. A Strict RGI match has a BLASTP bit-score above the curated BLASTP cutoff value and contains at least one curated mutation from amongst the mapped resistance variants, while a Loose RGI match has a bit-score less than the curated BLASTP bit-score cut-off but still contains at least one curated mutation from amongst the mapped resistance variants.
Bit-score Cut-off (blastP): 650
PubMed: mutation data hand curated from the scientific literature, evaluated as conferring resistance (R). CRyPTIC: mutation data acquired from the CRyPTIC catalog, evaluated as resistant (R), susceptible (S), or undetermined (U). ReSeqTB: mutation data acquired from the ReSeqTB catalog, evaluated as conferring resistance (Minimal, Moderate, High), not conferring resistance (None), or Indeterminate. WHO: mutation data acquired from the WHO 2023 catalog, evaluated as resistant (R), susceptible (S), or undetermined (U).| Mutation | Mutation type | PubMed |
|---|---|---|
| G141E | single resistance variant | PMID:10639355 |
| G141D | single resistance variant | PMID:10639355 |
| R154D | single resistance variant | PMID:10639355 |
| R154A | single resistance variant | PMID:10639355 |
| Curator | Description | Most Recent Edit |
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