APH(2'')-If

Accession ARO:3004191
DefinitionAminoglycoside 2''-phosphotransferase identified from the gram-negative pathogen Campylobacter jejuni. APH(2'')-If was shown to confer resistance to 4,6-disubstituted antibiotics kanamycin, tobramycin, dibekacin, gentamicin and sisomicin through antibiotic phosphorylation. Described by Toth et al. 2013.
AMR Gene FamilyAPH(2'')
Drug Classaminoglycoside antibiotic
Resistance Mechanismantibiotic inactivation
Resistomes with Perfect MatchesCampylobacter colig+p+wgs, Campylobacter jejunig+p+wgs, Clostridioides difficileg+wgs
Resistomes with Sequence VariantsCampylobacter colig+p+wgs, Campylobacter jejunig+p+wgs, Clostridioides difficileg+wgs
Classification19 ontology terms | Show
Parent Term(s)6 ontology terms | Show
+ confers_resistance_to_antibiotic dibekacin [Antibiotic]
+ confers_resistance_to_antibiotic sisomicin [Antibiotic]
+ confers_resistance_to_antibiotic kanamycin A [Antibiotic]
+ confers_resistance_to_antibiotic tobramycin [Antibiotic]
+ APH(2'') [AMR Gene Family]
+ confers_resistance_to_antibiotic gentamicin A [Antibiotic]
Publications

Toth M, et al. 2013. Antimicrob. Agents Chemother. 57(1):452-7 Novel aminoglycoside 2''-phosphotransferase identified in a gram-negative pathogen. (PMID 23129050)

Resistomes

Prevalence of APH(2'')-If among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 88 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
Campylobacter coli2.78%1.49%2.08%
Campylobacter jejuni1.14%3.53%1.57%
Clostridioides difficile4.62%0%2.86%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: The protein homolog model is an AMR detection model. Protein homolog models detect a protein sequence based on its similarity to a curated reference sequence. A protein homolog model has only one parameter: a curated BLASTP bitscore cutoff for determining the strength of a match. Protein homolog model matches to reference sequences are categorized on three criteria: perfect, strict and loose. A perfect match is 100% identical to the reference sequence along its entire length; a strict match is not identical but the bitscore of the matched sequence is greater than the curated BLASTP bitscore cutoff. Loose matches are other sequences with a match bitscore less than the curated BLASTP bitscore.

Bit-score Cut-off (blastP): 500


>gb|AAW34150.1|+|APH(2'')-If [Campylobacter jejuni]
MDIKKIIEEKCNIVVDSIKLIGEGYDSKAYIVNNEYVFKIKFSANKKKGYEKEKAIYDFLNKKLNTNIKIPNIEYSYISEELSILGYKEI
KGTFLTPEIYFALSKEKQELLKQDIAMFLRQMHDLDYSEISSYTIDNKQNVLEEYQLLKETIYDSLTDIEKQYVEEFMQRLNSTTIFDGK
KCLCHNDFSCNHLLLDDENRLCGVIDFGDSGIIDEYCDFIYLLEDSEEEIGVSFGEDILRLYGNIDISKAKEYQDVVEQYYPIETIVYGI
KNNRPDFIEKGRKEIYIRTRKDEKLRK


>gb|AY701528.1|+|9696-10589|APH(2'')-If [Campylobacter jejuni]
ATGGATATAAAAAAGATAATAGAAGAAAAATGCAATATAGTTGTTGATAGTATAAAGTTGATTGGTGAGGGTTATGACAGCAAAGCATAC
ATTGTAAATAATGAATATGTTTTCAAAATCAAATTTAGTGCTAATAAGAAAAAAGGGTATGAAAAAGAAAAAGCAATATATGATTTTCTA
AACAAAAAATTAAATACAAATATTAAAATACCAAATATAGAATATTCATATATAAGTGAAGAATTATCTATTTTAGGATATAAAGAAATT
AAAGGAACTTTTTTAACACCAGAAATATATTTTGCCTTATCAAAAGAAAAGCAAGAATTATTAAAGCAAGATATTGCTATGTTTTTAAGA
CAAATGCACGATTTAGATTATAGTGAAATAAGTTCATATACGATAGACAATAAACAAAATGTTTTAGAAGAATATCAATTACTTAAAGAA
ACAATATATGATAGTCTTACTGATATTGAAAAACAATATGTAGAAGAATTTATGCAAAGATTAAATAGTACAACTATATTTGATGGTAAA
AAATGCTTATGCCATAATGATTTTAGTTGTAATCATTTATTACTTGATGATGAAAATAGATTATGTGGTGTAATAGATTTTGGAGATTCT
GGAATTATAGATGAATACTGTGATTTCATATATTTGCTAGAAGATAGTGAAGAAGAAATTGGCGTGTCTTTTGGAGAAGATATATTAAGA
TTATACGGAAATATTGATATTTCAAAAGCAAAGGAATATCAAGATGTTGTAGAACAATATTATCCAATAGAAACTATTGTATATGGTATT
AAAAATAATAGACCTGATTTTATAGAAAAAGGTAGAAAAGAGATTTATATAAGAACTCGCAAAGATGAAAAATTAAGGAAGTGA