| Accession | ARO:3004332 |
| CARD Short Name | MCR-5.1 |
| Definition | MCR-5 is a transposon-associated phosphoethanolamine transferase gene, identified in Salmonella Paratyphi B dTa+ (d-tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B) isolates from food-producing animals. The isolates were collected between 2011 and 2013, and retrieved from the German National Reference Laboratory for Salmonella. MCR-5 confers resistance to collistin through the addition of a phosphoethanolamine group to lipid A, causing a reduction in negative charge of the cell membrane. Described by Borowiak et al, 2017. |
| AMR Gene Family | MCR phosphoethanolamine transferase |
| Drug Class | peptide antibiotic |
| Resistance Mechanism | antibiotic target alteration |
| Resistomes with Perfect Matches | Aeromonas hydrophilap, Enterobacter kobeiwgs, Escherichia colig+p+wgs, Pseudomonas aeruginosag, Salmonella entericap+wgs |
| Resistomes with Sequence Variants | Aeromonas hydrophilap, Aeromonas veroniip, Enterobacter kobeiwgs, Escherichia colig+p+wgs, Morganella morganiip, Pseudomonas aeruginosag, Salmonella entericap+wgs |
| Classification | 14 ontology terms | Show + process or component of antibiotic biology or chemistry + antibiotic molecule + peptide antibiotic [Drug Class] + mechanism of antibiotic resistance + lipopeptide antibiotic + antibiotic target alteration [Resistance Mechanism] + charge alteration conferring antibiotic resistance + determinant of antibiotic resistance + polymyxin antibiotic + gene altering cell wall charge + colistin + phosphoethanolamine transferase conferring colistin resistance + colistin B [Antibiotic] + colistin A [Antibiotic] |
| Parent Term(s) | 3 ontology terms | Show + confers_resistance_to_antibiotic colistin A [Antibiotic] + confers_resistance_to_antibiotic colistin B [Antibiotic] + MCR phosphoethanolamine transferase [AMR Gene Family] |
| Sub-Term(s) | 1 ontology terms | Show + MCR-5.2 evolutionary_variant_of |
| Publications | Borowiak M, et al. 2017. J. Antimicrob. Chemother. : Identification of a novel transposon-associated phosphoethanolamine transferase gene, mcr-5, conferring colistin resistance in d-tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B. (PMID 28962028) Partridge SR, et al. 2018. J. Antimicrob. Chemother. 73(10):2625-2630 Proposal for assignment of allele numbers for mobile colistin resistance (mcr) genes. (PMID 30053115) |
Prevalence of MCR-5.1 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 413 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI |
|---|---|---|---|---|
| Aeromonas hydrophila | 0% | 1.3% | 0% | 0% |
| Aeromonas veronii | 0% | 3.08% | 0% | 0% |
| Enterobacter kobei | 0% | 0% | 0.44% | 0% |
| Escherichia coli | 0.02% | 0.01% | 0.16% | 0% |
| Morganella morganii | 0% | 2.5% | 0% | 0% |
| Pseudomonas aeruginosa | 0.15% | 0% | 0% | 0% |
| Salmonella enterica | 0% | 0.05% | 0.03% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 1000