MCR-5.1

Accession ARO:3004332
CARD Short NameMCR-5.1
DefinitionMCR-5 is a transposon-associated phosphoethanolamine transferase gene, identified in Salmonella Paratyphi B dTa+ (d-tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B) isolates from food-producing animals. The isolates were collected between 2011 and 2013, and retrieved from the German National Reference Laboratory for Salmonella. MCR-5 confers resistance to collistin through the addition of a phosphoethanolamine group to lipid A, causing a reduction in negative charge of the cell membrane. Described by Borowiak et al, 2017.
AMR Gene FamilyMCR phosphoethanolamine transferase
Drug Classpeptide antibiotic
Resistance Mechanismantibiotic target alteration
Resistomes with Perfect MatchesAeromonas hydrophilap, Enterobacter kobeiwgs, Escherichia colig+p+wgs, Pseudomonas aeruginosag, Salmonella entericap+wgs
Resistomes with Sequence VariantsAeromonas hydrophilap, Aeromonas veroniip, Enterobacter kobeiwgs, Escherichia colig+p+wgs, Morganella morganiip, Pseudomonas aeruginosag, Salmonella entericap+wgs
Classification14 ontology terms | Show
Parent Term(s)3 ontology terms | Show
+ confers_resistance_to_antibiotic colistin A [Antibiotic]
+ confers_resistance_to_antibiotic colistin B [Antibiotic]
+ MCR phosphoethanolamine transferase [AMR Gene Family]
Sub-Term(s)
1 ontology terms | Show
+ MCR-5.2 evolutionary_variant_of
Publications

Borowiak M, et al. 2017. J. Antimicrob. Chemother. : Identification of a novel transposon-associated phosphoethanolamine transferase gene, mcr-5, conferring colistin resistance in d-tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B. (PMID 28962028)

Partridge SR, et al. 2018. J. Antimicrob. Chemother. 73(10):2625-2630 Proposal for assignment of allele numbers for mobile colistin resistance (mcr) genes. (PMID 30053115)

Resistomes

Prevalence of MCR-5.1 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GIGRDI-AMR2
Aeromonas hydrophila0%1.3%0%0%0%
Aeromonas veronii0%3.08%0%0%0%
Enterobacter kobei0%0%0.44%0%0%
Escherichia coli0.02%0.01%0.16%0%0%
Morganella morganii0%2.5%0%0%0%
Pseudomonas aeruginosa0.15%0%0%0%0%
Salmonella enterica0%0.05%0.03%0%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 1000


>gb|ASK40551.1|-|MCR-5.1 [Salmonella enterica subsp. enterica serovar Paratyphi B]
MRLSAFITFLKMRPQVRTEFLTLFISLVFTLLCNGVFWNALLAGRDSLTSGTWLMLLCTGLLITGLQWLLLLLVATRWSVKPLLILLAVM
TPAAVYFMRNYGVYLDKAMLRNLMETDVREASELLQWRMLPYLLVAAVSVWWIARVRVLRTGWKQAVMMRSACLAGALAMISMGLWPVMD
VLIPTLRENKPLRYLITPANYVISGIRVLTEQASSSADEAREVVAADAHRGPQEQGRRPRALVLVVGETVRAANWGLSGYERQTTPELAA
RDVINFSDVTSCGTDTATSLPCMFSLNGRRDYDERQIRRRESVLHVLNRSDVNILWRDNQSGCKGVCDGLPFENLSSAGHPTLCHGERCL
DEILLEGLAEKITTSRSDMLIVLHMLGNHGPAYFQRYPASYRRWSPTCDTTDLASCSHEALVNTYDNAVLYTDHVLARTIDLLSGIRSHD
TALLYVSDHGESLGEKGLYLHGIPYVIAPDEQIKVPMIWWQSSQVYADQACMQTHASRAPVSHDHLFHTLLGMFDVKTAAYTPELDLLAT
CRKGQPQ


>gb|KY807920.1|-|9896-11539|MCR-5.1 [Salmonella enterica subsp. enterica serovar Paratyphi B]
ATGCGGTTGTCTGCATTTATCACTTTCTTGAAAATGCGCCCGCAAGTGCGCACTGAATTTTTGACTCTGTTCATCAGCCTTGTGTTCACC
CTGCTGTGCAATGGCGTGTTTTGGAATGCCCTTCTTGCTGGACGCGACTCCCTAACTTCTGGAACATGGCTAATGCTCCTTTGCACTGGG
TTGCTGATCACCGGGCTGCAATGGTTGTTGCTCCTTCTGGTGGCCACGCGCTGGAGTGTCAAGCCACTACTGATTCTGCTTGCTGTCATG
ACGCCCGCCGCCGTTTATTTCATGCGCAACTACGGGGTTTATCTCGACAAGGCCATGCTGCGGAATCTGATGGAGACGGACGTCAGGGAA
GCCAGTGAGCTGTTGCAATGGAGAATGCTGCCCTACTTGTTGGTTGCAGCCGTATCCGTGTGGTGGATTGCGAGAGTCAGGGTTTTACGA
ACGGGCTGGAAACAAGCGGTAATGATGCGCAGCGCTTGTCTGGCTGGCGCTCTCGCCATGATTTCCATGGGTCTGTGGCCAGTCATGGAT
GTGCTGATACCCACGCTTCGTGAAAACAAGCCGCTTCGCTATTTGATCACTCCTGCAAACTACGTCATCTCGGGCATTCGGGTTTTGACT
GAACAGGCGTCATCGTCAGCAGACGAAGCAAGGGAAGTCGTTGCAGCCGATGCGCATCGAGGGCCTCAAGAACAAGGCCGCCGTCCTCGT
GCTCTCGTACTGGTTGTCGGGGAAACCGTCAGGGCGGCTAATTGGGGGTTGAGCGGCTATGAACGACAAACCACCCCTGAGTTGGCCGCA
CGCGACGTGATCAATTTTTCCGATGTCACCAGTTGCGGGACGGATACGGCTACATCCCTTCCCTGCATGTTTTCCCTCAATGGTCGGCGC
GACTACGACGAACGCCAGATTCGTCGGCGCGAGTCCGTGCTGCACGTTTTAAACCGTAGTGACGTCAACATTCTCTGGCGCGATAACCAG
TCGGGCTGTAAAGGCGTCTGTGATGGACTGCCCTTTGAAAACCTGTCTTCGGCAGGCCATCCCACACTGTGCCATGGCGAGCGCTGCCTG
GATGAAATTCTGCTCGAAGGGTTGGCCGAGAAGATAACAACAAGCCGCAGCGATATGCTGATCGTTCTGCATATGCTGGGCAATCACGGC
CCAGCGTATTTCCAGCGCTATCCCGCAAGCTACCGACGCTGGTCGCCAACCTGCGACACCACCGATCTGGCCAGCTGTTCGCATGAAGCC
TTGGTGAACACCTACGACAACGCCGTGCTTTACACCGATCATGTGCTTGCCCGTACCATTGACCTGCTGTCCGGCATCCGCTCACACGAC
ACGGCGCTGCTGTACGTTTCCGATCATGGGGAATCGCTCGGCGAGAAAGGCCTGTATCTCCATGGCATACCTTACGTCATCGCGCCGGAT
GAGCAGATCAAGGTGCCGATGATCTGGTGGCAGTCGAGTCAGGTTTATGCCGACCAAGCCTGTATGCAAACTCATGCCTCTCGGGCACCG
GTAAGTCACGATCACCTGTTTCACACCTTGCTCGGGATGTTCGACGTGAAAACCGCTGCCTACACGCCAGAGTTGGACCTTCTGGCAACA
TGCAGAAAAGGACAACCACAATGA

Curator Acknowledgements
Curator Description Most Recent Edit