| Accession | ARO:3004675 |
| CARD Short Name | aphA15 |
| Definition | Expression of cloned aphA15 gene in Escherichia coli reduced the susceptibility to kanamycin and neomycin, as well as to amikacin, netilmicin, and streptomycin. |
| AMR Gene Family | APH(3') |
| Drug Class | aminoglycoside antibiotic |
| Resistance Mechanism | antibiotic inactivation |
| Resistomes with Perfect Matches | Aeromonas veroniiwgs, Citrobacter freundiiwgs, Citrobacter portucalensiswgs, Enterobacter asburiaewgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheip+wgs, Enterobacter kobeip, Enterobacter roggenkampiiwgs, Escherichia colip+wgs, Klebsiella aerogenesp+wgs, Klebsiella pneumoniaep+wgs, Pseudomonas aeruginosawgs |
| Resistomes with Sequence Variants | Aeromonas veroniiwgs, Citrobacter freundiiwgs, Citrobacter portucalensiswgs, Enterobacter asburiaewgs, Enterobacter cloacaep+wgs, Enterobacter hormaecheip+wgs, Enterobacter kobeip, Enterobacter roggenkampiiwgs, Escherichia colip+wgs, Klebsiella aerogenesp+wgs, Klebsiella pneumoniaep+wgs, Lysobacter oculig, Pseudomonas aeruginosawgs, Pseudomonas putidawgs |
| Classification | 11 ontology terms | Show + process or component of antibiotic biology or chemistry + mechanism of antibiotic resistance + determinant of antibiotic resistance + antibiotic inactivation [Resistance Mechanism] + antibiotic inactivation enzyme + aminoglycoside modifying enzyme + phosphorylation of antibiotic conferring resistance + antibiotic molecule + aminoglycoside phosphotransferase (APH) + aminoglycoside antibiotic [Drug Class] + APH(3') [AMR Gene Family] |
| Parent Term(s) | 6 ontology terms | Show + confers_resistance_to_antibiotic neomycin [Antibiotic] + confers_resistance_to_antibiotic amikacin [Antibiotic] + confers_resistance_to_antibiotic netilmicin [Antibiotic] + confers_resistance_to_antibiotic streptomycin [Antibiotic] + confers_resistance_to_antibiotic kanamycin A [Antibiotic] + APH(3')-I |
| Publications | Riccio ML, et al. 2001. Antimicrob. Agents Chemother. 45(4):1249-53 In70 of plasmid pAX22, a bla(VIM-1)-containing integron carrying a new aminoglycoside phosphotransferase gene cassette. (PMID 11257042) |
Prevalence of aphA15 among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).
| Species | NCBI Chromosome | NCBI Plasmid | NCBI WGS | NCBI GI | GRDI-AMR2 |
|---|---|---|---|---|---|
| Aeromonas veronii | 0% | 0% | 0.56% | 0% | 0% |
| Citrobacter freundii | 0% | 0% | 1.35% | 0% | 0% |
| Citrobacter portucalensis | 0% | 0% | 0.9% | 0% | 0% |
| Enterobacter asburiae | 0% | 0% | 2.37% | 0% | 0% |
| Enterobacter cloacae | 0% | 0.56% | 0.96% | 0% | 0% |
| Enterobacter hormaechei | 0% | 0.06% | 0.91% | 0% | 0% |
| Enterobacter kobei | 0% | 0.69% | 0% | 0% | 0% |
| Enterobacter roggenkampii | 0% | 0% | 1.08% | 0% | 0% |
| Escherichia coli | 0% | 0.01% | 0.05% | 0% | 0% |
| Klebsiella aerogenes | 0% | 1.09% | 0.28% | 0% | 0% |
| Klebsiella pneumoniae | 0% | 0.03% | 0.12% | 0% | 0% |
| Lysobacter oculi | 100% | 0% | 0% | 0% | 0% |
| Pseudomonas aeruginosa | 0% | 0% | 1.52% | 0% | 0% |
| Pseudomonas putida | 0% | 0% | 0.53% | 0% | 0% |
Model Type: protein homolog model
Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.
Bit-score Cut-off (blastP): 500
| Curator | Description | Most Recent Edit |
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