tet(32)

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Accession ARO:3000196
Synonym(s)tet32
CARD Short Nametet(32)
DefinitionTet(32) is a tetracycline resistance gene similar to Tet(O), and binds to the ribosome to confer tetracycline resistance as a ribosomal protection protein.
AMR Gene Familytetracycline-resistant ribosomal protection protein
Drug Classtetracycline antibiotic
Resistance Mechanismantibiotic target protection
Resistomes with Perfect MatchesLachnospiraceae bacteriumgi, Ruthenibacterium lactatiformanswgs, Streptococcus anginosusg+wgs, Streptococcus pseudopneumoniaewgs, Streptococcus suiswgs
Resistomes with Sequence VariantsCampylobacter concisusg, Collinsella aerofacienswgs, Enterocloster clostridioformiswgs, Enterococcus faecalisg, Erysipelatoclostridium ramosumwgs, Eubacterium limosumg+gi, Faecalibacterium prausnitziiwgs, Lachnospiraceae bacteriumgi, Massilistercora timonensisg, Roseburia hominiswgs, Ruthenibacterium lactatiformanswgs, Streptococcus anginosusg+wgs, Streptococcus constellatusg, Streptococcus intermediuswgs, Streptococcus pneumoniaeg+wgs, Streptococcus pseudopneumoniaewgs, Streptococcus suiswgs, Treponema denticolag
Classification7 ontology terms | Show
Parent Term(s)7 ontology terms | Show
+ tetracycline-resistant ribosomal protection protein [AMR Gene Family]
+ confers_resistance_to_antibiotic tetracycline [Antibiotic]
+ confers_resistance_to_antibiotic doxycycline [Antibiotic]
+ confers_resistance_to_antibiotic minocycline [Antibiotic]
+ confers_resistance_to_antibiotic chlortetracycline [Antibiotic]
+ confers_resistance_to_antibiotic demeclocycline [Antibiotic]
+ confers_resistance_to_antibiotic oxytetracycline [Antibiotic]
Publications

Warburton P, et al. 2008. Antimicrob Agents Chemother 53(1): 273-276. Characterization of tet(32) genes from the oral metagenome. (PMID 18955517)

Melville CM, et al. 2001. Antimicrob Agents Chemother 45(11): 3246-3249. Novel tetracycline resistance gene, tet(32), in the Clostridium-related human colonic anaerobe K10 and its transmission in vitro to the rumen anaerobe Butyrivibrio fibrisolvens. (PMID 11600392)
Resistomes

Prevalence of tet(32) among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 414 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GIGRDI-AMR2
Campylobacter concisus6.25%0%0%0%0%
Collinsella aerofaciens0%0%1.49%0%0%
Enterocloster clostridioformis0%0%25.58%0%0%
Enterococcus faecalis0.45%0%0%0%0%
Erysipelatoclostridium ramosum0%0%4.65%0%0%
Escherichia coli0%0%0%0%0%
Eubacterium limosum25%0%0%100%0%
Faecalibacterium prausnitzii0%0%0.97%0%0%
Lachnospiraceae bacterium0%0%0%33.33%0%
Massilistercora timonensis100%0%0%0%0%
Roseburia hominis0%0%11.11%0%0%
Ruthenibacterium lactatiformans0%0%20%0%0%
Streptococcus anginosus5.88%0%2.92%0%0%
Streptococcus constellatus25%0%0%0%0%
Streptococcus intermedius0%0%4.55%0%0%
Streptococcus pneumoniae0.49%0%0.02%0%0%
Streptococcus pseudopneumoniae0%0%0.85%0%0%
Streptococcus suis0%0%0.26%0%0%
Treponema denticola6.67%0%0%0%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 300


>gb|ACH87088.1|+|tet(32) [Escherichia coli]
MKIINIGILAHVDAGKTTLTESLLYTSGAIAEQGNVDKGTTRTDTMILERQRGITIQTAVTSFYWNDYKINIVDTPGHMDFLTEAYRSLS
VLDGAVLVISAKDGVQAQTRILFHALQKMDIPTIIFINKIDQNGIDLQCVYQSIKDKLTSDMIVMQEVSLSPKITMTDISDLDKWDMIIS
GSDELLERYVAEDSLDIQELQYEKCKRTRCCSLFPVYHGSAKDNLGTEKLIEAIIETFITETDDIQSELCGYVFKVEYTERKKRLSYLRL
YHGTLHLRDTLLLSKKEKIKITEMCIPSNGEIVPADHACPGEIVILADDTLKLNDILGNEKLLPHKTRIDNPMPLLRTTVEPQKPEQREA
LLNALAEIADTDPLLHFDIDTVTHEIMLSFLGKVQLEVICSLLEEKYHVGVAMKEPSVIYLERPQKKASCTIHIEVPPNPFWASIGLTVT
PLPVGSGTQYKSEVSLGYLNQSFQNAVMEGVRYGMEQGLYGWGVTDCQICFDYGVYYSPVSTPADFRFLAPVVLEQALKKAGTQLLEPYL
SFTLFAPQEYLSRAYNDAPKYCAIIESTRLEKDEVIFKGEIPARCIGEYRNDLNFYTNGRSVCITELKGYQETSGEPVFQPRRPNSRLDK
IRHMFQKIM


>gb|EU722333.1|+|1933-3852|tet(32) [Escherichia coli]
ATGAAAATAATCAATATCGGCATTCTGGCCCATGTAGACGCAGGAAAAACTACATTGACAGAAAGTCTGCTATACACCAGTGGAGCGATT
GCGGAACAGGGAAACGTGGATAAAGGGACCACAAGAACAGACACTATGATTTTGGAACGGCAACGGGGAATTACCATTCAGACAGCGGTT
ACTTCTTTTTATTGGAATGATTATAAAATCAATATCGTGGACACTCCCGGTCATATGGATTTTTTAACCGAAGCATACCGCTCTTTATCT
GTCCTTGACGGAGCTGTTTTAGTCATTTCAGCAAAAGACGGCGTACAGGCACAAACCCGTATATTATTCCATGCGCTTCAGAAAATGGAC
ATTCCGACAATTATCTTTATAAATAAGATAGACCAAAATGGGATCGACCTGCAGTGTGTTTACCAAAGCATTAAAGATAAACTTACCAGT
GATATGATTGTCATGCAGGAGGTTTCCCTGTCGCCAAAGATAACCATGACCGATATTTCTGATTTAGACAAATGGGATATGATTATTTCC
GGAAGCGATGAACTATTAGAACGATATGTTGCAGAGGATTCTTTGGATATACAGGAATTACAATATGAAAAGTGCAAAAGAACCAGATGC
TGCTCTTTGTTTCCTGTTTATCATGGGAGTGCAAAAGACAATTTAGGAACAGAAAAACTGATTGAAGCGATTATAGAAACTTTCATTACA
GAAACGGACGATATTCAGTCTGAATTATGTGGATATGTTTTTAAGGTTGAGTATACAGAGCGGAAAAAACGGCTTTCTTATTTACGCCTG
TATCATGGGACGCTCCATTTACGGGATACCCTGCTGCTGTCAAAAAAGGAAAAAATAAAGATTACAGAAATGTGTATTCCGTCAAATGGT
GAAATCGTCCCGGCTGACCATGCCTGTCCGGGAGAAATTGTTATTTTAGCTGATGATACTTTGAAACTGAACGATATCCTGGGAAATGAA
AAACTCCTGCCTCACAAAACACGGATTGATAATCCCATGCCATTACTTCGGACAACGGTAGAGCCGCAAAAGCCGGAGCAAAGGGAAGCC
CTGTTAAATGCCCTCGCAGAGATTGCTGATACAGACCCTCTTTTGCATTTTGACATTGATACTGTTACCCATGAGATTATGTTATCTTTT
TTGGGAAAAGTACAGTTAGAAGTTATTTGTTCGCTATTAGAAGAAAAATATCATGTGGGCGTGGCTATGAAAGAGCCTTCGGTTATTTAT
CTGGAAAGACCGCAAAAAAAAGCGAGCTGCACGATTCATATTGAAGTGCCGCCGAATCCGTTTTGGGCATCTATTGGTTTGACTGTAACA
CCGCTTCCTGTTGGAAGCGGAACACAATATAAAAGCGAGGTATCTCTCGGCTATTTAAACCAAAGTTTTCAAAATGCCGTCATGGAGGGT
GTGCGTTATGGAATGGAGCAGGGCTTATATGGCTGGGGAGTGACAGACTGCCAAATTTGTTTTGATTATGGAGTTTATTACAGCCCGGTC
AGCACCCCCGCTGATTTTCGTTTTCTTGCGCCTGTCGTGTTGGAGCAGGCATTGAAAAAAGCAGGGACACAACTGTTGGAACCATACCTT
TCCTTTACCCTTTTTGCACCGCAGGAATATCTTTCACGGGCTTATAATGATGCACCAAAGTATTGCGCAATCATTGAATCAACCAGACTT
GAAAAAGATGAAGTTATTTTTAAGGGTGAAATCCCTGCCCGTTGTATCGGTGAATATAGGAATGATCTGAATTTTTATACAAATGGAAGA
AGTGTCTGCATTACAGAATTAAAAGGGTATCAGGAAACTTCCGGCGAGCCTGTATTTCAGCCACGCCGCCCGAACAGCCGTTTAGACAAG
ATCCGGCATATGTTTCAGAAGATAATGTAA

Curator Acknowledgements
Curator Description Most Recent Edit