APH(3')-VIa

Accession ARO:3002652
Synonym(s)aphA-6
CARD Short NameAPH(3')-VIa
DefinitionAPH(3')-VIa is a plasmid-encoded aminoglycoside phosphotransferase in A. baumannii.
AMR Gene FamilyAPH(3')
Drug Classaminoglycoside antibiotic
Resistance Mechanismantibiotic inactivation
Resistomes with Perfect MatchesAcinetobacter baumanniig+p+wgs, Acinetobacter defluviip, Acinetobacter haemolyticusp, Acinetobacter indicusg, Acinetobacter johnsoniip+wgs, Acinetobacter juniip, Acinetobacter lwoffiip, Acinetobacter nosocomialisp+wgs, Acinetobacter pittiip+wgs, Acinetobacter radioresistenswgs, Acinetobacter wuhouensisp, Enterobacter roggenkampiiwgs, Escherichia colip+wgs, Klebsiella pneumoniaewgs, Klebsiella quasipneumoniaewgs, Pseudomonas aeruginosag+wgs, Pseudomonas putidawgs
Resistomes with Sequence VariantsAcinetobacter baumanniig+p+wgs, Acinetobacter defluviip, Acinetobacter haemolyticusp+wgs, Acinetobacter indicusg+p+wgs, Acinetobacter johnsoniip+wgs, Acinetobacter juniip, Acinetobacter lwoffiip, Acinetobacter nosocomialisp+wgs, Acinetobacter pittiip+wgs, Acinetobacter radioresistenswgs, Acinetobacter wuhouensisp, Actinobacillus pleuropneumoniaeg, Aeromonas caviaeg, Alcaligenes faecalisp, Burkholderia cepaciawgs, Citrobacter freundiiwgs, Citrobacter portucalensiswgs, Citrobacter werkmaniiwgs, Enterobacter hormaecheip+wgs, Enterobacter kobeiwgs, Enterobacter roggenkampiiwgs, Escherichia colig+p+wgs, Klebsiella michiganensiswgs, Klebsiella pneumoniaep+wgs, Klebsiella quasipneumoniaep+wgs, Pasteurella multocidawgs, Proteus mirabilisg+p+wgs, Providencia rettgeriwgs, Pseudomonas aeruginosag+wgs, Pseudomonas monteiliiwgs, Pseudomonas putidawgs, Salmonella entericawgs, Serratia marcescenswgs, Stenotrophomonas maltophiliag+wgs, Vibrio parahaemolyticuswgs
Classification13 ontology terms | Show
Parent Term(s)9 ontology terms | Show
+ confers_resistance_to_antibiotic isepamicin [Antibiotic]
+ confers_resistance_to_antibiotic butirosin [Antibiotic]
+ confers_resistance_to_antibiotic ribostamycin [Antibiotic]
+ confers_resistance_to_antibiotic gentamicin B [Antibiotic]
+ confers_resistance_to_antibiotic paromomycin [Antibiotic]
+ confers_resistance_to_antibiotic kanamycin A [Antibiotic]
+ confers_resistance_to_antibiotic amikacin [Antibiotic]
+ confers_resistance_to_antibiotic neomycin [Antibiotic]
+ APH(3')-VI
Publications

Martin P, et al. 1988. Mol Microbiol 2(5): 615-625. Nucleotide sequence of Acinetobacter baumannii aphA-6 gene: evolutionary and functional implications of sequence homologies with nucleotide-binding proteins, kinases and other aminoglycoside-modifying enzymes. (PMID 2846986)

Resistomes

Prevalence of APH(3')-VIa among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI or IslandViewer for 377 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, genome islands, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein homolog model (view sequences)

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGSNCBI GI
Acinetobacter baumannii4.57%2.96%17.96%0%
Acinetobacter defluvii0%12.5%0%0%
Acinetobacter haemolyticus0%2.44%2.44%0%
Acinetobacter indicus4.76%15.09%2.78%0%
Acinetobacter johnsonii0%4.17%4%0%
Acinetobacter junii0%33.33%0%0%
Acinetobacter lwoffii0%1.49%0%0%
Acinetobacter nosocomialis0%1.64%3.25%0%
Acinetobacter pittii0%3.16%3.29%0%
Acinetobacter radioresistens0%0%4.88%0%
Acinetobacter wuhouensis0%4.55%0%0%
Actinobacillus pleuropneumoniae3.03%0%0%0%
Aeromonas caviae3.23%0%0%0%
Alcaligenes faecalis0%25%0%0%
Burkholderia cepacia0%0%0.45%0%
Citrobacter freundii0%0%0.24%0%
Citrobacter portucalensis0%0%1.72%0%
Citrobacter werkmanii0%0%2.56%0%
Enterobacter hormaechei0%0.39%0.41%0%
Enterobacter kobei0%0%0.52%0%
Enterobacter roggenkampii0%0%0.4%0%
Escherichia coli0.03%0.02%0.03%0%
Klebsiella michiganensis0%0%0.3%0%
Klebsiella pneumoniae0%0.14%0.38%0%
Klebsiella quasipneumoniae0%0.27%0.16%0%
Pasteurella multocida0%0%1.65%0%
Proteus mirabilis3.3%1.49%2.39%0%
Providencia rettgeri0%0%0.74%0%
Pseudomonas aeruginosa2.06%0%0.94%0%
Pseudomonas monteilii0%0%7.5%0%
Pseudomonas putida0%0%2.74%0%
Salmonella enterica0%0%0.02%0%
Serratia marcescens0%0%2.34%0%
Stenotrophomonas maltophilia1.37%0%0.17%0%
Vibrio parahaemolyticus0%0%0.06%0%
Show Perfect Only


Detection Models

Model Type: protein homolog model

Model Definition: Protein Homolog Models (PHM) detect protein sequences based on their similarity to a curated reference sequence, using curated BLASTP bitscore cut-offs. Protein Homolog Models apply to all genes that confer resistance through their presence in an organism, such as the presence of a beta-lactamase gene on a plasmid. PHMs include a reference sequence and a bitscore cut-off for detection using BLASTP. A Perfect RGI match is 100% identical to the reference protein sequence along its entire length, a Strict RGI match is not identical but the bit-score of the matched sequence is greater than the curated BLASTP bit-score cutoff, Loose RGI matches have a bit-score less than the curated BLASTP bit-score cut-off.

Bit-score Cut-off (blastP): 500


>gb|CAA30578.1|+|APH(3')-VIa [Acinetobacter baumannii]
MELPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSSTLYTETTYSVSREAKMLSWLSEKLKVPELIMTFQDEQFEFMITKAI
NAKPISALFLTDQELLAIYKEALNLLNSIAIIDCPFISNIDHRLKESKFFIDNQLLDDIDQDDFDTELWGDHKTYLSLWNELTETRVEER
LVFSHGDITDSNIFIDKFNEIYFLDLGRAGLADEFVDISFVERCLREDASEETAKIFLKHLKNDRPDKRNYFLKLDELN


>gb|X07753.1|+|103-882|APH(3')-VIa [Acinetobacter baumannii]
ATGGAATTGCCCAATATTATTCAACAATTTATCGGAAACAGCGTTTTAGAGCCAAATAAAATTGGTCAGTCGCCATCGGATGTTTATTCT
TTTAATCGAAATAATGAAACTTTTTTTCTTAAGCGATCTAGCACTTTATATACAGAGACCACATACAGTGTCTCTCGTGAAGCGAAAATG
TTGAGTTGGCTCTCTGAGAAATTAAAGGTGCCTGAACTCATCATGACTTTTCAGGATGAGCAGTTTGAATTCATGATCACTAAAGCGATC
AATGCAAAACCAATTTCAGCGCTTTTTTTAACAGACCAAGAATTGCTTGCTATCTATAAGGAGGCACTCAATCTGTTAAATTCAATTGCT
ATTATTGATTGTCCATTTATTTCAAACATTGATCATCGGTTAAAAGAGTCAAAATTTTTTATTGATAACCAACTCCTTGACGATATAGAT
CAAGATGATTTTGACACTGAATTATGGGGAGACCATAAAACTTACCTAAGTCTATGGAATGAGTTAACCGAGACTCGTGTTGAAGAAAGA
TTGGTTTTTTCTCATGGCGATATCACGGATAGTAATATTTTTATAGATAAATTCAATGAAATTTATTTTTTAGATCTTGGTCGTGCTGGG
TTAGCAGATGAATTTGTAGATATATCCTTTGTTGAACGTTGCCTAAGAGAGGATGCATCGGAGGAAACTGCGAAAATATTTTTAAAGCAT
TTAAAAAATGATAGACCTGACAAAAGGAATTATTTTTTAAAACTTGATGAATTGAATTGA