Bartonella bacilliformis gyrA conferring resistance to fluoroquinolones

Accession ARO:3003297
DefinitionPoint mutation of Bartonella bacilliformis gyrA resulted in the lowered affinity between fluoroquinolones and gyrA. Thus, conferring resistance.
AMR Gene Familyfluoroquinolone resistant gyrA
Drug Classfluoroquinolone antibiotic
Resistance Mechanismantibiotic target alteration
Classification12 ontology terms | Show
Parent Term(s)14 ontology terms | Show
+ fluoroquinolone resistant gyrA [AMR Gene Family]
+ confers_resistance_to_antibiotic ciprofloxacin [Antibiotic]
+ confers_resistance_to_antibiotic enoxacin [Antibiotic]
+ confers_resistance_to_antibiotic gatifloxacin [Antibiotic]
+ confers_resistance_to_antibiotic grepafloxacin [Antibiotic]
+ confers_resistance_to_antibiotic levofloxacin [Antibiotic]
+ confers_resistance_to_antibiotic lomefloxacin [Antibiotic]
+ confers_resistance_to_antibiotic moxifloxacin [Antibiotic]
+ confers_resistance_to_antibiotic nalidixic acid [Antibiotic]
+ confers_resistance_to_antibiotic norfloxacin [Antibiotic]
+ confers_resistance_to_antibiotic ofloxacin [Antibiotic]
+ confers_resistance_to_antibiotic pefloxacin [Antibiotic]
+ confers_resistance_to_antibiotic sparfloxacin [Antibiotic]
+ confers_resistance_to_antibiotic trovafloxacin [Antibiotic]
Publications

Minnick MF, et al. 2002. Antimicrob Agents Chemother 47(1): 383-386. gyrA mutations in ciprofloxacin-resistant Bartonella bacilliformis strains obtained in vitro. (PMID 12499219)

Resistomes

Prevalence of Bartonella bacilliformis gyrA conferring resistance to fluoroquinolones among the sequenced genomes, plasmids, and whole-genome shotgun assemblies available at NCBI for 82 important pathogens (see methodological details and complete list of analyzed pathogens). Values reflect percentage of genomes, plasmids, or whole-genome shotgun assemblies that have at least one hit to the AMR detection model. Default view includes percentages calculated based on Perfect plus Strict RGI hits. Select the checkbox to view percentages based on only Perfect matches to AMR reference sequences curated in CARD (note: this excludes resistance via mutation as references in protein variant models are often wild-type, sensitive sequences).

Prevalence: protein variant model

SpeciesNCBI ChromosomeNCBI PlasmidNCBI WGS
No prevalence data


Detection Models

Model Type: protein variant model

Model Definition: The protein variant model is an AMR detection model. Protein variant models are similar to protein homolog models - they detect the presence of a protein sequence based on its similarity to a curated reference sequence, but secondarily search submitted query sequences for curated sets of mutations shown clinically to confer resistance relative to wild-type. This model includes a protein reference sequence, a curated BLASTP cut-off, and mapped resistance variants. Mapped resistance variants may include any or all of: single resistance variants, insertions, deletions, co-dependent resistance variants, nonsense SNPs, and/or frameshift mutations. Protein variant model matches to reference sequences are categorized on two criteria: strict and loose. A strict match has a BLASTP bitscore above the curated BLASTP cutoff value and contains at least one detected mutation from amongst the mapped resistance variants; a loose match has a BLASTP bitscore below the curated BLASTP cutoff value but still contains at least one detected mutation from amongst the mapped resistance variants. Regardless of BLASTP bitscore, if a sequence does not contain one of the mapped resistance variants, it is not considered a match and not detected by the protein variant model.

Legend:

  • discovered in clinical, agricultural, or environmental isolates
  • discovered via laboratory selection experiments


Bit-score Cut-off (blastP): 1500

PMID: 12499219D90G D95N

>gb|AAL82403.1|+|Bartonella bacilliformis gyrA conferring resistance to fluoroquinolones [Bartonella bacilliformis]
MTDLTRLPEHDVSTGIEPVSIIEEMQCSYLDYAMSVIVSRALPDVRDGLKPVHRRILHAM
NEMGLLFNKPYRKSAGVVGEVMGKFHPHGDASIYDALVRMAQDFSLRNPLIDGQGNFGSV
DGDPPAAMRYTECRLEKVAEELLADIDKDTVDFQDNYDGREHEPIVLPARFPNLLVNGSG
GIAVGMATNIPPHNLGEVIDGCVALIDNPNITIDEMLAIIPGPDFPTGGIILGHSGVRSA
YETGRGSIIMRAKVEIEEIRNQRQAIIVSEIPYQVNKATMVEKMAELVRDKRIEGISDLR
DESDRDGYRVVIELKREAVADVVLNQLYRYTPLQASFGCNMVALNGGKPEQMTLLDMLRA
FVSFREEVVSRRTKYLLRKARERAHVLVGLAIAVANIDEIIELIRKAHDPQTARTQLMER
RWPASEVAALIKLIDDPRHIIHEDNTYNLSEEQARAILELRLQRLTALGRNEIADELNAI
GEDIADYLGILASRSRIMDIVKSELSALRETFATPRRTVFGFGSAEMDCEDLIVPEDMVV
TVSHSGYIKRVPLNTYRAQRRGGKGRSGMATKDQDFVTRLFVANTHTPVLFFSSRGIVYK
EKVWRLPVGTPQSRGRALINMLPLQQGERITTIMPLPEDEASWGKLDIMFATTRGTVRRN
KLSDFIQVNRNGKIAMKLDEEGDEILSVETCTEHDDVVLITANGQCIRFPVTDIRVFSGR
NSMGVRGINMVEGDKVISMTILEHVEATSVERSAYIKRAINERRVAGSDDEDILTVDEDG
EETEVELTDERYAELSAHEQMLLTVSEFGYGKRSSSYDFRISGRGGKGIRATDLSKAAEI
GKLVAAFPVGERDQIMLVSDGGQLIRVPVNCIRIAGRSTKGVTVFNTAKGEKVVSVERIS
ESENDTNQLDIESEEHSGTVSMSEEKKL



>gb|AF469609|+|567-3353|Bartonella bacilliformis gyrA conferring resistance to fluoroquinolones [Bartonella bacilliformis]
GTGACCGATCTTACTCGACTACCAGAACATGATGTGTCGACCGGTATTGAACCAGTCAGTATCATTGAAGAAATGCAGTGCTCTTATCTA
GATTATGCGATGAGCGTAATTGTGTCGCGCGCACTGCCTGATGTCCGTGATGGGCTTAAGCCTGTCCATCGGCGCATTCTTCATGCGATG
AATGAAATGGGACTTTTGTTCAATAAGCCTTATCGTAAGTCAGCGGGTGTTGTTGGTGAAGTGATGGGAAAGTTTCATCCTCATGGTGAT
GCTTCAATTTATGATGCCTTGGTGCGTATGGCACAGGATTTTTCTTTACGAAATCCTCTGATTGATGGACAGGGAAATTTTGGCTCTGTT
GACGGTGATCCACCCGCAGCGATGCGTTACACGGAATGTCGTTTAGAAAAAGTTGCAGAAGAACTTTTAGCTGATATTGATAAAGATACT
GTTGATTTTCAAGATAATTATGATGGGCGTGAGCATGAACCTATAGTTTTGCCTGCACGTTTCCCTAACCTGTTAGTAAATGGATCGGGT
GGTATTGCTGTAGGTATGGCAACCAATATTCCTCCACATAATCTAGGTGAGGTTATTGATGGATGTGTTGCTTTGATCGATAATCCTAAC
ATAACTATAGATGAGATGTTAGCAATTATTCCGGGGCCTGATTTTCCTACAGGTGGTATTATTCTTGGCCATTCTGGTGTCCGTTCTGCT
TATGAAACAGGGCGTGGTTCAATTATTATGCGTGCTAAGGTTGAGATCGAGGAAATTCGCAATCAGCGGCAGGCAATTATCGTAAGCGAA
ATACCTTATCAAGTTAATAAAGCAACAATGGTTGAGAAGATGGCCGAATTGGTGCGTGATAAACGTATCGAAGGAATCTCCGATTTGCGT
GATGAATCTGATCGTGATGGGTATCGAGTTGTCATTGAGCTAAAAAGAGAAGCTGTTGCAGACGTTGTTTTGAATCAGCTTTATCGTTAT
ACACCGTTGCAAGCCTCATTTGGTTGCAATATGGTTGCGTTGAATGGAGGAAAGCCTGAACAAATGACGTTGCTTGACATGCTTCGTGCA
TTTGTTTCCTTCCGCGAAGAAGTGGTAAGCCGGCGCACAAAATATCTTTTGCGTAAAGCACGTGAGCGTGCGCATGTTTTAGTTGGTCTT
GCTATCGCTGTTGCTAATATTGATGAGATTATAGAATTAATTCGCAAAGCTCATGATCCACAGACAGCGCGTACACAGTTAATGGAACGG
CGTTGGCCGGCTTCTGAGGTAGCAGCTTTGATTAAGCTTATAGATGATCCTCGTCATATTATTCATGAGGATAATACGTACAATTTGTCT
GAAGAACAAGCGCGTGCTATTTTAGAATTGCGTTTGCAAAGATTAACAGCGCTTGGTCGTAATGAAATTGCTGATGAACTCAATGCAATT
GGAGAAGATATTGCTGACTATCTTGGTATTTTAGCATCACGCTCACGGATCATGGACATTGTTAAAAGTGAGCTCAGCGCTTTGCGTGAA
ACATTTGCAACACCACGGCGTACTGTATTTGGTTTTGGTAGTGCCGAGATGGACTGCGAAGATCTGATTGTTCCAGAAGATATGGTGGTG
ACAGTGAGCCATAGTGGCTATATTAAGCGTGTGCCTCTAAATACATACCGTGCGCAGCGTCGTGGTGGTAAGGGACGTTCTGGTATGGCA
ACAAAGGATCAGGATTTTGTTACTCGCTTATTCGTGGCCAATACACATACACCAGTTCTTTTCTTTTCATCACGTGGGATTGTTTATAAA
GAGAAGGTTTGGCGTTTACCTGTTGGTACGCCGCAATCACGCGGTAGAGCTTTAATTAATATGTTGCCTTTGCAACAAGGCGAGCGCATT
ACAACAATTATGCCATTGCCGGAGGATGAGGCAAGTTGGGGTAAACTGGATATTATGTTTGCAACAACGCGTGGAACTGTGCGCCGTAAT
AAATTATCAGACTTTATTCAAGTTAATCGCAATGGTAAAATAGCAATGAAACTTGATGAAGAGGGAGATGAGATCCTTTCTGTAGAGACC
TGTACAGAACATGATGACGTTGTTCTTATTACAGCAAACGGGCAGTGTATTCGTTTTCCAGTTACTGATATTCGTGTATTTTCTGGTCGT
AATTCGATGGGGGTACGCGGTATCAATATGGTTGAGGGTGATAAGGTCATTTCAATGACTATTTTAGAGCATGTTGAAGCGACATCAGTC
GAACGTTCTGCTTATATTAAACGTGCAATCAATGAGCGTCGTGTTGCAGGTTCAGATGATGAAGATATTTTAACTGTTGATGAAGATGGA
GAGGAAACTGAGGTTGAATTAACAGATGAACGTTATGCAGAACTTAGCGCTCATGAGCAAATGCTTTTAACAGTTAGTGAATTTGGTTAT
GGAAAACGCTCTTCTTCTTATGATTTCCGTATTTCAGGACGCGGTGGAAAAGGGATACGCGCAACTGATCTATCAAAGGCGGCTGAAATT
GGTAAGTTAGTAGCAGCCTTTCCAGTAGGAGAACGAGATCAAATTATGTTAGTTTCGGATGGAGGACAGCTTATTCGTGTTCCCGTCAAC
TGTATTCGTATAGCGGGTCGTTCAACTAAAGGGGTCACAGTCTTTAATACAGCAAAAGGTGAAAAAGTTGTATCGGTTGAGCGTATTTCT
GAATCTGAAAATGATACTAATCAGTTAGATATTGAAAGTGAGGAACATTCCGGAACAGTTAGCATGAGTGAAGAGAAAAAACTCTGA